7-Aminoactinomycin D - ≥97.0% (HPLC), high purity , CAS No.7240-37-1

Item Number

A131295

• Synonyms: 7-amino-AMD | CHEBI:52304 | UNII-DXS32PH95R | Actinomycin D, 7-Amino- - CAS 7240-37-1 | PD165780 | Actinomycin D, 7-amino- | MFCD00077295 | 7-Aminoaktinomycin D | 7-AAD | Q259277 | 2,7-diamino-4,6-dimethyl-3-oxo-1-N,9-N-bis-[(18aS)-10c,14,17-trimethyl-5,8
• Specifications & Purity: ≥97%(HPLC)
• Biochemical and Physiological Mechanisms: Fluorescent DNA dye for apoptosis and flow cytometry studies. Guanine-specific synthetic fluorescent analogue of actinomycin D. Intercalates DNA by binding to GC regions. Discriminates between live cells as well as early and late apoptosis in flow cytomet
• Storage Temp: Protected from light,Store at -20°C
• Shipped In: Ice chest + Ice pads
• Note: Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Product Description

7-Aminoactinomycin D ，an analog of the antibiotic actinomycin D ，is a fluorescent DNA dye which is useful for quantifying apoptosis. 7-AAD has also been used in studies on cellular necrosis, chromosome analysis and in cell cycle studies. As a fluorescent DNA dye, 7-AAD is able to intercalate DNA via binding to the GC regions. When used in flow cytometery, 7-AAD can be used to discriminate between live cells as well as early and late apoptosis. Additionally, 7-AAD can also be combined with other staining procedures for analysis of intracellular and cell surface antigens enabling multiparametric analysis. When utilizing 7-AAD with flow cytometery, early and late apoptosis can be studied by varying the concentration of 7-AAD. During apoptosis, the cellular plasma membrane is altered progressively, which allows the cell membrane to become permeable to 7-AAD. 7-AAD staining can also be adapted to include staining with other dyes for intracellular and cell surface antigens. 7-Aminoactinomycin D is an inhibitor of DNA Polymerase and RNA Polymerase
A fluorescent DNA dye for apoptosis and flow cytomtery studies.