ABCB5 Mouse mAb

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
  • Clone Number: CHS11A2
  • Conjugation: Unconjugated
Item Number
Ab086955
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab086955-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab086955-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$149.90
Ab086955-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$249.90
Ab086955-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (CHS11A2); Mouse anti Human ABCB5 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameABCB5 Mouse mAb
SynonymsABCB 5 antibody | ABCB5 antibody | ABCB5 P-gp antibody | ABCB5_HUMAN antibody | ABCB5alpha antibody | ABCB5beta antibody | ATP binding cassette protein antibody | ATP binding cassette sub family B (MDR/TAP) member 5 antibody | ATP binding cassette sub fam
Specifications & PurityCarrier Free, ExactAb™, Validated, Lot by Lot
Biochemical and Physiological MechanismsEnergy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.
Host speciesMouse
SpecificityABCB5
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence of human ABCB5.
Positive ControlWB: HeLa, HEK293 and Jurkat cell lysates. IHC: Human breast cancer tissue.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Mouse anti Human ABCB5 Antibody, Monoclonal (CHS11A2), could be used for WB, IHC and so on.

Product Properties

IsotypeMouse IgG1
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

ABCB5 Mouse mAb (Ab086955) - Western Blot
All lanes: ABCB5 Mouse mAb (Ab086955) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 86 kDa
Exposure time: 100.0 s

ABCB5 Mouse mAb (Ab086955) - IHC
Immunohistochemical analysis of ABCB5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ABCB5 Mouse mAb (Ab086955) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Associated Targets(Human)

ABCB5 Tbio ATP-binding cassette sub-family B member 5 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/1000 - 1/2000

">

1/1000 - 1/2000

IHC

1/200-1/500

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Related Documents

Solution Calculators