pAb; Rabbit anti Human ACAT1 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab087103-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab087103-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $754.90 | |
| Ab087103-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $169.90 | |
| Ab087103-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $279.90 | |
| Ab087103-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
pAb; Rabbit anti Human ACAT1 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | ACAT1 Antibody - Primary antibody, specific to ACAT1, Rabbit IgG |
|---|---|
| Synonyms | ACAT 1 antibody; ACAT antibody; acat1 antibody; Acetoacetyl CoA thiolase antibody; acetoacetyl Coenzyme A thiolase antibody; Acetoacetyl-CoA thiolase antibody; Acetyl CoA acetyltransferase, mitochondrialantibody; Acetyl Coenzyme A acetyltransferase 1 anti |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Lot by Lot |
| Host species | Rabbit |
| Specificity | ACAT1 |
| Immunogen | Recombinant ACAT1 expressed in E.coli (AA 111-427) |
| Positive Control | WB: A549, HEK293, A431, MOLT-4, Jurkat, Hep G2 and NIH/3T3 cell lysates. IHC: Human Kidney Tissue, Human Lymph node Tissue. ICC/IF: Hep G2 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human ACAT1 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen-specific affinity chromatography followed by Protein A affinity chromatography. |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
ACAT1 Antibody (Ab087103) - Western Blot
All lanes: ACAT1 Antibody (Ab087103) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 40 kDa
Exposure time: 19.8 s
ACAT1 Antibody (Ab087103) - IF
FITC staining on IF
Samples: Human Hep G2 cells
Primary Ab: 20μg/mL ACAT1 Antibody (Ab087103)
Second Ab: 1.5μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ACAT1 Antibody (Ab087103) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20μg/mL ACAT1 Antibody (Ab087103)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ACAT1 Antibody (Ab087103) - IHC
DAB staining on IHC
Samples: Human Lymph node Tissue
Primary Ab: 20μg/mL ACAT1 Antibody (Ab087103)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB | 0.5-2 µg/mL |
| ICC/IF |
5-20 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901022 | Certificate of Analysis | Sep 11, 2023 | Ab087103 |
| ZJ23F0901021 | Certificate of Analysis | Sep 11, 2023 | Ab087103 |
| 1. Haapalainen AM, Meriläinen G, Pirilä PL, Kondo N, Fukao T, Wierenga RK. (2007) Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function.. Biochemistry, 46 (14): (4305-21). [PMID:17371050] |
| 2. Fukao T, Yamaguchi S, Orii T, Schutgens RB, Osumi T, Hashimoto T. (1992) Identification of three mutant alleles of the gene for mitochondrial acetoacetyl-coenzyme A thiolase. A complete analysis of two generations of a family with 3-ketothiolase deficiency.. J Clin Invest, 89 (2): (474-9). [PMID:1346617] |
| 3. Fukao T, Yamaguchi S, Tomatsu S, Orii T, Frauendienst-Egger G, Schrod L, Osumi T, Hashimoto T. (1991) Evidence for a structural mutation (347Ala to Thr) in a German family with 3-ketothiolase deficiency.. Biochem Biophys Res Commun, 179 (1): (124-9). [PMID:1715688] |
| 4. Wakazono A, Fukao T, Yamaguchi S, Hori T, Orii T, Lambert M, Mitchell GA, Lee GW, Hashimoto T. (1995) Molecular, biochemical, and clinical characterization of mitochondrial acetoacetyl-coenzyme A thiolase deficiency in two further patients.. Hum Mutat, 5 (1): (34-42). [PMID:7728148] |
| 5. Fukao T, Nakamura H, Song XQ, Nakamura K, Orii KE, Kohno Y, Kano M, Yamaguchi S, Hashimoto T, Orii T et al.. (1998) Characterization of N93S, I312T, and A333P missense mutations in two Japanese families with mitochondrial acetoacetyl-CoA thiolase deficiency.. Hum Mutat, 12 (4): (245-54). [PMID:9744475] |
| 6. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 7. Ikenoya M, Yoshinaka Y, Kobayashi H, Kawamine K, Shibuya K, Sato F, Sawanobori K, Watanabe T, Miyazaki A. (2007) A selective ACAT-1 inhibitor, K-604, suppresses fatty streak lesions in fat-fed hamsters without affecting plasma cholesterol levels.. Atherosclerosis, 191 (2): (290-7). [PMID:16820149] |
| 8. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 9. Kano, M M and 5 more authors.. (1991) Structure and expression of the human mitochondrial acetoacetyl-CoA thiolase-encoding gene.. Gene, (30): [PMID:1684944] |
| 10. Fukao, T T and 6 more authors.. (1990) Molecular cloning and sequence of the complementary DNA encoding human mitochondrial acetoacetyl-coenzyme A thiolase and study of the variant enzymes in cultured fibroblasts from patients with 3-ketothiolase deficiency.. The Journal of clinical investigation, [PMID:1979337] |
| 11. Fukao, T T, Yamaguchi, S S, Orii, T T and Hashimoto, T T.. (1995) Molecular basis of beta-ketothiolase deficiency: mutations and polymorphisms in the human mitochondrial acetoacetyl-coenzyme A thiolase gene.. Human mutation, [PMID:7749408] |
| 12. Aboulaich, Nabila N, Vainonen, Julia P JP, Strålfors, Peter P and Vener, Alexander V AV.. (2004) Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes.. The Biochemical journal, (15): [PMID:15242332] |
| 13. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 14. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 15. Ibeas Bih, Clementino C and 5 more authors.. (2015) Molecular Targets of Cannabidiol in Neurological Disorders.. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, [PMID:26264914] |
| 16. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
