| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab087545-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab087545-20μl | 20μl | In stock | $79.90 | |
| Ab087545-50μl | 50μl | In stock | $159.90 | |
| Ab087545-100μl | 100μl | In stock | $209.90 | |
| Ab087545-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,799.90 |
mAb (H2); Mouse anti Human Adiponectin Antibody; WB, IHC; Unconjugated
| Product Name | Adiponectin Mouse mAb - Primary antibody, specific to ADIPOQ, Mouse IgG2b |
|---|---|
| Synonyms | 30 kDa adipocyte complement related protein antibody | 30 kDa adipocyte complement-related protein antibody | ACDC antibody | Acrp 30 antibody | ACRP30 antibody | ADIPO_HUMAN antibody | Adipocyte antibody | Adipocyte C1q and collagen domain containing pro |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | ADIPOQ |
| Immunogen | Recombinant ADPN expressed in E.coli (AA 108-244) |
| Positive Control | WB: Mouse skeletal muscle lysate. IHC: Human cardiac muscle, Rat cardiac muscle tissues. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Note | 20μl卖完停产，不再备货 |
| Product Description | Mouse anti Human Adiponectin Antibody, Monoclonal (H2), could be used for WB, IHC and so on. Application: WB: 0.01-2µg/mL IHC: 5-30µg/mL Protein Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Adiponectin Mouse mAb (Ab087545) - Western Blot
All lanes: Adiponectin Mouse mAb (Ab087545) at 1/2000 dilution
Samples: Lysates at 5 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa
Exposure time: 50.0 s
Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Rat Cardiac Muscle Tissue
Primary Ab: 30 µg/mL Adiponectin Mouse mAb (Ab087545)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1201914 | Certificate of Analysis | Dec 06, 2023 | Ab087545 |
| ZJ23F1201913 | Certificate of Analysis | Dec 06, 2023 | Ab087545 |
| ZJ23F1201912 | Certificate of Analysis | Dec 06, 2023 | Ab087545 |
| 1. Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K. (1996) cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1).. Biochem Biophys Res Commun, 221 (2): (286-9). [PMID:8619847] [10.1021/op500134e] |
| 2. Nakano, Y Y, Tobe, T T, Choi-Miura, N H NH, Mazda, T T and Tomita, M M.. (1996) Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma.. Journal of biochemistry, [PMID:8947845] |
| 3. Saito, K K and 8 more authors.. (1999) Organization of the gene for gelatin-binding protein (GBP28).. Gene, (18): [PMID:10095105] |
| 4. Schäffler, A A and 7 more authors.. (1999) The human apM-1, an adipocyte-specific gene linked to the family of TNF's and to genes expressed in activated T cells, is mapped to chromosome 1q21.3-q23, a susceptibility locus identified for familial combined hyperlipidaemia (FCH).. Biochemical and biophysical research communications, (5): [PMID:10403784] |
| 5. Takahashi, M M and 13 more authors.. (2000) Genomic structure and mutations in adipose-specific gene, adiponectin.. International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity, [PMID:10918532] |
| 6. Yokota, T T and 10 more authors.. (2000) Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages.. Blood, (1): [PMID:10961870] |
| 7. Ouchi, N N and 14 more authors.. (2000) Adiponectin, an adipocyte-derived plasma protein, inhibits endothelial NF-kappaB signaling through a cAMP-dependent pathway.. Circulation, (12): [PMID:10982546] |
| 8. Yamauchi, T T and 24 more authors.. (2001) The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity.. Nature medicine, [PMID:11479627] |
| 9. Hara, Kazuo K and 23 more authors.. (2002) Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population.. Diabetes, [PMID:11812766] |
| 10. Kondo, Hidehiko H and 11 more authors.. (2002) Association of adiponectin mutation with type 2 diabetes: a candidate gene for the insulin resistance syndrome.. Diabetes, [PMID:12086969] |
| 11. Vasseur, Francis F and 14 more authors.. (2002) Single-nucleotide polymorphism haplotypes in the both proximal promoter and exon 3 of the APM1 gene modulate adipocyte-secreted adiponectin hormone levels and contribute to the genetic risk for type 2 diabetes in French Caucasians.. Human molecular genetics, (1): [PMID:12354786] |
| 12. Waki, Hironori H and 12 more authors.. (2003) Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin.. The Journal of biological chemistry, (10): [PMID:12878598] |
| 13. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 14. Richards, Ayanthi A AA and 6 more authors.. (2006) Adiponectin multimerization is dependent on conserved lysines in the collagenous domain: evidence for regulation of multimerization by alterations in posttranslational modifications.. Molecular endocrinology (Baltimore, Md.), [PMID:16497731] |
| 15. Richards, Ayanthi A AA and 13 more authors.. (2010) Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation.. Molecular endocrinology (Baltimore, Md.), [PMID:19855092] |
| 16. Min, Xiaoshan X and 7 more authors.. (2012) Crystal structure of a single-chain trimer of human adiponectin globular domain.. FEBS letters, (23): [PMID:22449980] |
| 17. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912] |
