Adiponectin Mouse mAb - Primary antibody, specific to ADIPOQ, Mouse IgG2b

Features and benefits
  • Short Overview:

    mAb (H2); Mouse anti Human Adiponectin Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: Mouse IgG2b
    Application:
  • IHC
  • WB
Item Number
Ab087545
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab087545-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$56.90
Ab087545-20μl
20μl
In stock
$79.90
Ab087545-50μl
50μl
In stock
$159.90
Ab087545-100μl
100μl
In stock
$209.90
Ab087545-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,799.90

mAb (H2); Mouse anti Human Adiponectin Antibody; WB, IHC; Unconjugated

Basic Description

Product NameAdiponectin Mouse mAb - Primary antibody, specific to ADIPOQ, Mouse IgG2b
Synonyms30 kDa adipocyte complement related protein antibody; 30 kDa adipocyte complement-related protein antibody; ACDC antibody; Acrp 30 antibody; ACRP30 antibody; ADIPO_HUMAN antibody; Adipocyte antibody; Adipocyte C1q and collagen domain containing protein an
Specifications & PurityExactAb™, Validated, Carrier Free, High performance, Lot by Lot
Host speciesMouse
SpecificityADIPOQ
ImmunogenRecombinant ADPN expressed in E.coli (AA 108-244)
Positive ControlWB: Mouse skeletal muscle lysate. IHC: Human cardiac muscle, Rat cardiac muscle tissues.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human Adiponectin Antibody, Monoclonal (H2), could be used for WB, IHC and so on.

Application:

WB: 0.01-2µg/mL IHC: 5-30µg/mL

Protein Function

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

Product Properties

IsotypeMouse IgG2b
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A + Protein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Adiponectin Mouse mAb (Ab087545) - Western Blot
All lanes: Adiponectin Mouse mAb (Ab087545) at 1/2000 dilution
Samples: Lysates at 5 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 26 kDa
Observed band size: 28 kDa
Exposure time: 50.0 s 

Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Rat Cardiac Muscle Tissue
Primary Ab: 30 µg/mL Adiponectin Mouse mAb (Ab087545)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Associated Targets(Human)

ADIPOQ Tbio Adiponectin (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

5-30 µg/mL

WB

0.01-2 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ23F1201914Certificate of AnalysisDec 06, 2023 Ab087545
ZJ23F1201913Certificate of AnalysisDec 06, 2023 Ab087545
ZJ23F1201912Certificate of AnalysisDec 06, 2023 Ab087545

Related Documents

References

1. Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K.  (1996)  cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1)..  Biochem Biophys Res Commun,  221  (2): (286-9).  [PMID:8619847]
2. Nakano, Y Y, Tobe, T T, Choi-Miura, N H NH, Mazda, T T and Tomita, M M..  (1996)  Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma..  Journal of biochemistry,      [PMID:8947845]
3. Saito, K K and 8 more authors..  (1999)  Organization of the gene for gelatin-binding protein (GBP28)..  Gene,    (18):   [PMID:10095105]
4. Schäffler, A A and 7 more authors..  (1999)  The human apM-1, an adipocyte-specific gene linked to the family of TNF's and to genes expressed in activated T cells, is mapped to chromosome 1q21.3-q23, a susceptibility locus identified for familial combined hyperlipidaemia (FCH)..  Biochemical and biophysical research communications,    (5):   [PMID:10403784]
5. Takahashi, M M and 13 more authors..  (2000)  Genomic structure and mutations in adipose-specific gene, adiponectin..  International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity,      [PMID:10918532]
6. Yokota, T T and 10 more authors..  (2000)  Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages..  Blood,    (1):   [PMID:10961870]
7. Ouchi, N N and 14 more authors..  (2000)  Adiponectin, an adipocyte-derived plasma protein, inhibits endothelial NF-kappaB signaling through a cAMP-dependent pathway..  Circulation,    (12):   [PMID:10982546]
8. Yamauchi, T T and 24 more authors..  (2001)  The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity..  Nature medicine,      [PMID:11479627]
9. Hara, Kazuo K and 23 more authors..  (2002)  Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population..  Diabetes,      [PMID:11812766]
10. Kondo, Hidehiko H and 11 more authors..  (2002)  Association of adiponectin mutation with type 2 diabetes: a candidate gene for the insulin resistance syndrome..  Diabetes,      [PMID:12086969]
11. Vasseur, Francis F and 14 more authors..  (2002)  Single-nucleotide polymorphism haplotypes in the both proximal promoter and exon 3 of the APM1 gene modulate adipocyte-secreted adiponectin hormone levels and contribute to the genetic risk for type 2 diabetes in French Caucasians..  Human molecular genetics,    (1):   [PMID:12354786]
12. Waki, Hironori H and 12 more authors..  (2003)  Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin..  The Journal of biological chemistry,    (10):   [PMID:12878598]
13. Zhang, Zemin Z and Henzel, William J WJ..  (2004)  Signal peptide prediction based on analysis of experimentally verified cleavage sites..  Protein science : a publication of the Protein Society,      [PMID:15340161]
14. Richards, Ayanthi A AA and 6 more authors..  (2006)  Adiponectin multimerization is dependent on conserved lysines in the collagenous domain: evidence for regulation of multimerization by alterations in posttranslational modifications..  Molecular endocrinology (Baltimore, Md.),      [PMID:16497731]
15. Richards, Ayanthi A AA and 13 more authors..  (2010)  Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation..  Molecular endocrinology (Baltimore, Md.),      [PMID:19855092]
16. Min, Xiaoshan X and 7 more authors..  (2012)  Crystal structure of a single-chain trimer of human adiponectin globular domain..  FEBS letters,    (23):   [PMID:22449980]
17. Rosenow, Anja A and 6 more authors..  (2012)  Resveratrol-induced changes of the human adipocyte secretion profile..  Journal of proteome research,    (7):   [PMID:22905912]

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