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ADIPOQ Mouse mAb - Primary antibody, for WB,IHC various applications, from Human, generated in Mouse, specific to ADIPOQ, Mouse IgG2b

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Features and benefits

Short Overview:
mAb (H2); Mouse anti Human ADIPOQ Antibody; WB, IHC; Unconjugated
Application: WB,IHC
Species reactivity(Reacts with): Human,Pig,Rat
Isotype: Mouse IgG2b

Application:

Item Number

Ab087546

Grouped product items
SKU	Size	Availability	Price
Ab087546-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$46.90
Ab087546-20μl	20μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab087546-50μl	50μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$138.90
Ab087546-100μl	100μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$198.90
Ab087546-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$657.90

mAb (H2); Mouse anti Human ADIPOQ Antibody; WB, IHC; Unconjugated

View related series

Click to view target information: ADIPOQ

Accession#:Q15848 Acrp30 Adiponectin ADIPOQ Gene ID:9370

Basic Description

Product Name	ADIPOQ Mouse mAb - Primary antibody, for WB,IHC various applications, from Human, generated in Mouse, specific to ADIPOQ, Mouse IgG2b
Synonyms	Adiponectin antibody; 30 kDa adipocyte complement related protein antibody; 30 kDa adipocyte complement-related protein antibody; ACDC antibody; Acrp 30 antibody; ACRP30 antibody; ADIPO_HUMAN antibody; Adipocyte antibody; Adipocyte C1q and collagen domain
Specifications & Purity	ExactAb™, Validated, Carrier Free, High performance, Lot by Lot
Host Species	Mouse
Host species	Mouse
Specificity	ADIPOQ
Application	WB,IHC
Immunogen	Recombinant ADIPOQ (Ala108~Asn244) expressed in E.coli
Positive Control	WB: K562, HeLa and C6 cell lysates. IHC: Human Cardiac Muscle Tissue.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, High Performance, Validated
Product Description	Mouse monoclonal (H2) to ADIPOQ could be used for WB, IHC and so on. Application WB: 0.29-2µg/mL; IHC: 5-20µg/mL; Protein Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Post-translational Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.

Product Properties

Antibody Type	Primary antibody
Clonality	Monoclonal antibodies
Isotype	Mouse IgG2b
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A + Protein G affinity chromatography.
Source	Hybridoma supernatant
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4 ℃ for frequent use. Aliquot and store at -20 ℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

ADIPOQ Mouse mAb (Ab087546) - Western Blot
All lanes: ADIPOQ Mouse mAb (Ab087546) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 26 kDa
Observed band size: 25, 27 kDa
Exposure time: 80.6 s

ADIPOQ Mouse mAb (Ab087546) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL ADIPOQ Mouse mAb (Ab087546)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Application

Dilution info

IHC

5-20 µg/mL

0.29-2µg/mL

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1001487	Certificate of Analysis	Nov 02, 2023	Ab087546
ZJ23F1001488	Certificate of Analysis	Nov 02, 2023	Ab087546

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