Aladdin™ EDC - Standard, high purity , CAS No.25952-53-8

Item Number

A638729

• Specifications & Purity: Standard
• Storage Temp: Store at -20°C,Desiccated
• Shipped In: Ice chest + Ice pads
• Grade: Standard

Product Description

EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins. 

Characteristics of EDC:
• Reactive group:carbodiimide
• Reaction target: activates carboxyl groups to conjugate to amino groups (primary amines)
• Several conjugation strategies—react EDC alone with target groups or includecarbodiimide
• Reactivity: Forms active intermediate with carboxyl groups at pH 4.7–6.0 (optimum), then intermediate reacts with primary amines