alpha-Smooth Muscle Actin Mouse mAb - Primary antibody, specific to Alpha smooth muscle Actin, Mouse IgG1

Features and benefits
  • Short Overview:

    mAb (F1); Mouse anti Human alpha-Smooth Muscle Actin Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Cow,Dog,Human,Mouse,Pig,Rat
  • Isotype: Mouse IgG1
    Application:
  • IHC
  • WB
Item Number
Ab088443
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab088443-10μl
10μl
In stock
$59.90
Ab088443-20μl
20μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab088443-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$177.90
Ab088443-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$297.90
Ab088443-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,007.90

mAb (F1); Mouse anti Human alpha-Smooth Muscle Actin Antibody; WB, IHC; Unconjugated

Basic Description

Product Namealpha-Smooth Muscle Actin Mouse mAb - Primary antibody, specific to Alpha smooth muscle Actin, Mouse IgG1
SynonymsA actin antibody; AAT6 antibody; ACTA_HUMAN antibody; ACTA2 antibody; Actin alpha 2 smooth muscle aorta antibody; Actin aortic smooth muscle antibody; Actin, aortic smooth muscle antibody; ACTSA antibody; ACTVS antibody; Alpha 2 actin antibody; Alpha acti
Specifications & PurityExactAb™, Validated, Carrier Free, High performance, Lot by Lot
Host speciesMouse
SpecificityAlpha smooth muscle Actin
ImmunogenRecombinant ACTA2 expressed in E.coli (AA 167-377).
Positive ControlWB: NIH/3T3, Mouse skeletal and Mouse heart lysates; IHC: Human Ovarian cancer tissues, Human Rectum tissue, Rat Skeletal muscle tissues, Rat Lung tissue, Mouse Pancreas tissues, Mouse Spleen tissues.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human Alpha smooth muscle Actin Antibody, Monoclonal (F1), could be used for WB, IHC and so on.
Application
WB:0.001-2 μg/mL
IHC: 5-20 µg/mL
Protein Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A + Protein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot
All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5.6 s

Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot
All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 26.7 s

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Human Ovarian cancer Tissue
Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse
IgG Polyclonal Antibody.

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Human Rectum Tissue
Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Rat Skeletal muscle Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Rat Lung Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Mouse Pancreas Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Mouse Spleen Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Associated Targets(Human)

ACTA2 Tbio Actin, aortic smooth muscle (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

5-20 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404290Certificate of AnalysisApr 09, 2024 Ab088443
ZJ23F0800857Certificate of AnalysisAug 22, 2023 Ab088443
ZJ23F0800856Certificate of AnalysisAug 22, 2023 Ab088443

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