ExactAb™, Validated, Carrier Free, High performance, Lot by Lot
Host species
Mouse
Specificity
Alpha smooth muscle Actin
Immunogen
Recombinant ACTA2 expressed in E.coli (AA 167-377).
Positive Control
WB: NIH/3T3, Mouse skeletal and Mouse heart lysates; IHC: Human Ovarian cancer tissues, Human Rectum tissue, Rat Skeletal muscle tissues, Rat Lung tissue, Mouse Pancreas tissues, Mouse Spleen tissues.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Product Description
Mouse anti Human Alpha smooth muscle Actin Antibody, Monoclonal (F1), could be used for WB, IHC and so on. Application WB:0.001-2 μg/mL IHC: 5-20 µg/mL Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Product Properties
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5.6 s
Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 26.7 s
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Human Ovarian cancer Tissue Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Human Rectum Tissue Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Rat Skeletal muscle Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Rat Lung Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Mouse Pancreas Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Mouse Spleen Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody