mAb (1B7D7); Mouse anti Human AMF Antibody; WB, IHC, ICC, IF, ELISA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab088587-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab088587-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $198.90 | |
| Ab088587-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $321.90 | |
| Ab088587-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,937.90 |
mAb (1B7D7); Mouse anti Human AMF Antibody; WB, IHC, ICC, IF, ELISA; Unconjugated
| Product Name | AMF Mouse mAb - Primary antibody, specific to AMF, IgG1 |
|---|---|
| Synonyms | AMF antibody; Aurocrine motility factor antibody; Autocrine motility factor antibody; DKFZp686C13233 antibody; EC 5.3.1.9 antibody; G6PI_HUMAN antibody; Glucose phosphate isomerase antibody; Glucose-6-phosphate isomerase antibody; GNPI antibody; GPI antib |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0mg/mL |
| Host species | Mouse |
| Specificity | AMF |
| Immunogen | Purified recombinant fragment of human GPI(AA:1-558) expressed in E.Coli. |
| Positive Control | WB: Hep G2, A431, K562, HeLa, A549, MCF-7 cell lysates. ICC/IF: L-02 cells. IHC: Human Kidney tissues. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Application: |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
AMF Mouse mAb (Ab088587) - Western Blot
All lanes: AMF Mouse mAb (Ab088587) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 57 kDa
AMF Mouse mAb (Ab088587) - ICC/IF
AMF Mouse mAb (Ab088587) at 1/1000 dilution staining AMF in L-02 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. Green staining in image show positive staining with AMF Mouse mAb, actin filaments were stained red with DY-554 phalloidin and nuclei stained blue with DRAQ5 fluorescent DNA dye.
AMF Mouse mAb (Ab088587) - IHC
Immunohistochemical analysis of paraffin-embedded human Kidney tissues using AMF Mouse mAb (Ab088587) .
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IF/ICC |
1/200-1/1000
|
| ELISA |
1/10000
|
| IHC |
1/200-1/1000
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700700 | Certificate of Analysis | Jul 26, 2023 | Ab088587 |
| ZJ23F0700701 | Certificate of Analysis | Jul 26, 2023 | Ab088587 |
