ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot
Host species
Rabbit
Specificity
ANGPT2
Immunogen
Recombinant ANGPT2 expressed in E.coli (AA 24-165)
Positive Control
WB: K562 and HUVEC cell lysates. IHC: Human Placenta Tissue
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description
Rabbit anti Human ANGPT2 Antibody, Polyclonal antibody, could be used for WB, IHC and so on. Application WB: 0.2-2µg/mL IHC: 5-20µg/mL Protein Function Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
ANGPT2 Antibody (Ab088801) - Western Blot All lanes: ANGPT2 Antibody (Ab088801) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 79 kDa Observed band size: 73 kDa Exposure time: 0.8 s
ANGPT2 Antibody (Ab088801) - IHC DAB staining on IHC Samples: Human Placenta Tissue Primary Ab: 20 μg/mL ANGPT2 Antibody (Ab088801) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.