Basic Description

Product Name	Annexin A1/ANXA1 Mouse mAb - Primary antibody, specific to ANXA1, Mouse IgG1
Synonyms	Annexin 1 antibodyAnnexin A1 antibody \| Annexin I (lipocortin I) antibody \| Annexin I antibody \| Annexin-1 antibody \| AnnexinA1 antibody \| AnnexinI antibody \| ANX 1 antibody \| ANX A1 antibody \| ANX1 antibody \| ANXA 1 antibody \| ANXA1 antibody \| ANXA1 prot
Specifications & Purity	ExactAb™, Validated, Carrier Free, 1.0mg/mL
Host species	Mouse
Specificity	ANXA1
Immunogen	Purified recombinant fragment of human ANXA1 (AA: 144-248) expressed in E. Coli.
Positive Control	WB: Hela, A549, K562, C6. IHC: Human cervical cancer tissue, Human ovarian cancer tissue.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Application: WB: 1/500 - 1/2000 IHC: 1/200 - 1/1000 ELISA: 1/10000 Protein Function Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	150kDa
Purification Method	Protein G purified
Form	Liquid
Concentration	1.0mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.

Images

Annexin A1/ANXA1 Mouse mAb (Ab088935) - Western Blot
All lanes: Annexin A1/ANXA1 Mouse mAb (Ab088935) at 1/1000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 37 kDa
Observed band size: 39 kDa

Annexin A1/ANXA1 Mouse mAb (Ab088935) - IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using Annexin A1/ANXA1 Mouse mAb (Ab088935) at a dilution of 1/200 with DAB staining.

Annexin A1/ANXA1 Mouse mAb (Ab088935) - IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue using Annexin A1/ANXA1 Mouse mAb (Ab088935) at a dilution of 1/200 with DAB staining.

Associated Targets(Human)

ANXA1 Tbio Annexin A1 (0 Activities)

Discover Target: ANXA1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/2000 "> 1/500-1/2000
ELISA	1/10000 "> 1/10000
IHC	1/200-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0700559	Certificate of Analysis	Jul 14, 2023	Ab088935
ZJ23F0700558	Certificate of Analysis	Jul 14, 2023	Ab088935

FAQs and Articles

References

1. Ernst S, Lange C, Wilbers A, Goebeler V, Gerke V, Rescher U. (2004) An annexin 1 N-terminal peptide activates leukocytes by triggering different members of the formyl peptide receptor family.. J Immunol, 172 (12): (7669-76). [PMID:15187149] [10.1021/op500134e]
2. Dorovkov MV, Ryazanov AG. (2004) Phosphorylation of annexin I by TRPM7 channel-kinase.. J Biol Chem, 279 (49): (50643-6). [PMID:15485879] [10.1021/op500134e]
3. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e]
4. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211]
5. Giovanna Leoni,Philipp-Alexander Neumann,Nazila Kamaly,Miguel Quiros,Hikaru Nishio,Hefin R Jones,Ronen Sumagin,Roland S Hilgarth,Ashfaqul Alam,Gabrielle Fredman,Ioannis Argyris,Emile Rijcken,Dennis Kusters,Chris Reutelingsperger,Mauro Perretti,Charles A Parkos,Omid C Farokhzad,Andrew S Neish,Asma Nusrat. (2015-02-11) Annexin A1-containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair.. The Journal of clinical investigation, 125 ((3)): (1215-1227). [PMID:25664854]
6. Kovacic, R T RT, Tizard, R R, Cate, R L RL, Frey, A Z AZ and Wallner, B P BP.. (1991) Correlation of gene and protein structure of rat and human lipocortin I.. Biochemistry, (17): [PMID:1832554]
7. Varticovski, L L and 7 more authors.. (1988) Location of sites in human lipocortin I that are phosphorylated by protein tyrosine kinases and protein kinases A and C.. Biochemistry, (17): [PMID:2457390]
8. Pepinsky, R B RB, Sinclair, L K LK, Chow, E P EP and O'Brine-Greco, B B.. (1989) A dimeric form of lipocortin-1 in human placenta.. The Biochemical journal, (1): [PMID:2532504]
9. Wallner, B P BP and 9 more authors.. (1986) Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity.. Nature, [PMID:2936963]
10. Biemann, K K and Scoble, H A HA.. (1987) Characterization by tandem mass spectrometry of structural modifications in proteins.. Science (New York, N.Y.), (28): [PMID:3303336]
11. Arcone, R R and 8 more authors.. (1993) Structural characterization of a biologically active human lipocortin 1 expressed in Escherichia coli.. European journal of biochemistry, (15): [PMID:8425544]
12. Weng, X X and 5 more authors.. (1993) Crystal structure of human annexin I at 2.5 A resolution.. Protein science : a publication of the Protein Society, [PMID:8453382]
13. Mailliard, W S WS, Haigler, H T HT and Schlaepfer, D D DD.. (1996) Calcium-dependent binding of S100C to the N-terminal domain of annexin I.. The Journal of biological chemistry, (12): [PMID:8557678]
14. Gao, J J, Li, Y Y and Yan, H H.. (1999) NMR solution structure of domain 1 of human annexin I shows an autonomous folding unit.. The Journal of biological chemistry, (29): [PMID:9915835]
15. Réty, S S and 7 more authors.. (2000) Structural basis of the Ca(2+)-dependent association between S100C (S100A11) and its target, the N-terminal part of annexin I.. Structure (London, England : 1993), (15): [PMID:10673436]
16. Morand, Eric F EF, Hall, Pam P, Hutchinson, Paul P and Yang, Yuan H YH.. (2006) Regulation of annexin I in rheumatoid synovial cells by glucocorticoids and interleukin-1.. Mediators of inflammation, [PMID:16883066]
17. D'Acquisto, Fulvio F and 7 more authors.. (2007) Annexin-1 modulates T-cell activation and differentiation.. Blood, (1): [PMID:17008549]
18. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
19. Goshima, Naoki N and 54 more authors.. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome,.. Nature methods, [PMID:19054851]
20. McArthur, Simon S and 6 more authors.. (2009) Annexin A1 regulates hormone exocytosis through a mechanism involving actin reorganization.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:19625660]
21. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
22. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569]
23. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O.. (2015) SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage.. Cell reports, (17): [PMID:25772364]
24. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

SKU	Size	Availability	Price
Ab088935-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$66.90
Ab088935-50μl	50μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$198.90
Ab088935-100μl	100μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$321.90
Ab088935-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,937.90

Annexin A1/ANXA1 Mouse mAb - Primary antibody, specific to ANXA1, Mouse IgG1