| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112146-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab112146-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab112146-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab112146-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
mAb (OTI14A11); Mouse anti Human Kindlin 2 Antibody; WB,IHC, IF/ICC, IP; Unconjugated
| Product Name | Anti-Kindlin 2 antibody |
|---|---|
| Synonyms | FLJ34213 | FLJ44462 | UNC112 | FERM2_HUMAN | Fermitin family homolog 2 | Fermt2 | KIND2 | Kindlin-2 | MIG-2 | MIG2 | Mitogen-inducible gene 2 protein | PH domain-containing family C member 1 | Pleckstrin homology domain containing family C (with FERM doma |
| Specifications & Purity | KO Validation, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. |
| Host species | Mouse |
| Specificity | FERMT2 |
| Immunogen | Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector |
| Positive Control | WB: Hep G2, HeLa, A549, NIH/3T3 and C6 cell lysates. IHC: Human pancreas tissues. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY FERMT2, Hep G2 cells. IP: HEK293T lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, KO Validation, Validated |
| Product Description |
Mouse anti Human Kindlin 2 Antibody, Monoclonal (OTI14A11), could be used for WB, IHC, IF/ICC, IP and so on.
|
| SDS-PAGE | 150 kDa |
|---|---|
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Kindlin 2 Mouse mAb (Ab112146) - Western Blot
All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/30000 dilution
Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 100.0 s
Kindlin 2 Mouse mAb (Ab112146) - Western Blot
All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 30.0 s
Kindlin 2 Mouse mAb (Ab112146) - KO
Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT) and FERMT2-Knockout HeLa cells (KO) were separated by SDSPAGE and immunoblotted with Kindlin 2 Mouse mAb (Ab112146) (1/500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Kindlin 2 Mouse mAb (Ab112146) - IF/ICC
Kindlin 2 Mouse mAb (Ab112146) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2.
Kindlin 2 Mouse mAb (Ab112146) - IF/ICC
Immunofluorescent staining of Hep G2 cells using Kindlin 2 Mouse mAb (Ab112146).
Kindlin 2 Mouse mAb (Ab112146) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using Kindlin 2 Mouse mAb (Ab112146). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
1/1000-1/2000
">
1/1000-1/2000
|
| IF/ICC | 1/50-1/100 |
| IHC | 1/50 |
| IP |
2ug/500ul
|
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