DNA polymerase α, δ and ε inhibitor. Tetracyclic diterpene antibiotic. Reversible inhibitor of eukaryotic nuclear DNA replication. It blocks the cell cycle at early S phase.
Storage Temp
Store at 2-8°C
Shipped In
Wet ice
Note
10mg 中国和美国售完停产 Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
Aphidicolin is a tetracyclic diterpene antibiotic and DNA synthesis inhibitor isolated from Cephalosporium aphidicola. Aphidicolin is reported to specifically inhibit DNA polymerase α and δ via binding to the enzyme, in eukaryotic cells such as the HeLa cell line, without affecting other DNA polymerases. This compound blocks the cell cycle at early S phase and has been reported to be useful for synchronizing cell cycles in cultured eukaryotic cells. This compound is also noted to induce apoptosis in HeLa S3 cells after 18-24 hours of the cell cycle. A novel tetracyclic diterpene antibiotic and also a DNA synthesis inhibitor in eukaryotes
Associated Targets(Human)
POLA1Tclin DNA polymerase alpha subunit (225 Activities)
Soluble in ethanol (1 mg/ml,stable at least a week at 4°C.), DMSO (10 mg/ml,stable at least six weeks at -20°C.), and methanol (10 mg/ml). Insoluble in water.
1.Wensen Zhang, Na Cui, Fazhi Su, Yanping Sun, Biao Li, Yupeng Ren, Ping Wang, Haodong Bai, Wei Guan, Bingyou Yang, Qiuhong Wang, Haixue Kuang. (2023) Serum, spleen metabolomics and gut microbiota reveals effect of catalpol on blood deficiency syndrome caused by cyclophosphamide and acetylphenylhydrazine. Frontiers in Immunology, 14 (29):(1280049). [PMID:38022670]
2.Wang Juan, Wang Furong, Yuan Lixia, Ruan Hongsheng, Zhu Zhibiao, Fan Xiaoling, Zhu Lingyan, Peng Xin. (2022) Blood-Enriching Effects and Immune-Regulation Mechanism of Steam-Processed Polygonatum Sibiricum Polysaccharide in Blood Deficiency Syndrome Mice. Frontiers in Immunology, 13 [PMID:35250989]
References
1.Xu Y et al.. (2017) 53BP1 and BRCA1 control pathway choice for stalled replication restart.. Elife, 6 [PMID:29106372]
2.Chen S et al.. (2020) Nonrandom DNA Segregation Detection under Replication Stress.. STAR Protoc, 1 (3):(100143). [PMID:33377037]
3.Grow EJ et al.. (2021) p53 convergently activates Dux/DUX4 in embryonic stem cells and in facioscapulohumeral muscular dystrophy cell models.. Nat Genet, 53 (8):(1207-1220). [PMID:34267371]
4.Hu X et al.. (2019) Region-Restrict Astrocytes Exhibit Heterogeneous Susceptibility to Neuronal Reprogramming.. Stem Cell Reports, 12 (2):(290-304). [PMID:30713039]
5.Teng Y et al.. (2018) ROS-induced R loops trigger a transcription-coupled but BRCA1/2-independent homologous recombination pathway through CSB.. Nat Commun, 9 (4115). [PMID:30297739]
6.Luo X et al.. (2018) SUN1 Regulates HIV-1 Nuclear Import in a Manner Dependent on the Interaction between the Viral Capsid and Cellular Cyclophilin A.. J Virol, 92 (13):[PMID:29643244]
7.Wang H et al.. (2020) Synergistic lethality between PARP-trapping and alantolactone-induced oxidative DNA damage in homologous recombination-proficient cancer cells.. Oncogene, 39 (14):(2905-2920). [PMID:32029902]
8.Watts LP et al.. (2020) The RIF1-long splice variant promotes G1 phase 53BP1 nuclear bodies to protect against replication stress.. Elife, 9 [PMID:33141022]
9.Wensen Zhang, Na Cui, Fazhi Su, Yanping Sun, Biao Li, Yupeng Ren, Ping Wang, Haodong Bai, Wei Guan, Bingyou Yang, Qiuhong Wang, Haixue Kuang. (2023) Serum, spleen metabolomics and gut microbiota reveals effect of catalpol on blood deficiency syndrome caused by cyclophosphamide and acetylphenylhydrazine. Frontiers in Immunology, 14 (29):(1280049). [PMID:38022670]
10.Wang Juan, Wang Furong, Yuan Lixia, Ruan Hongsheng, Zhu Zhibiao, Fan Xiaoling, Zhu Lingyan, Peng Xin. (2022) Blood-Enriching Effects and Immune-Regulation Mechanism of Steam-Processed Polygonatum Sibiricum Polysaccharide in Blood Deficiency Syndrome Mice. Frontiers in Immunology, 13 [PMID:35250989]
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