| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab089282-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab089282-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $189.90 | |
| Ab089282-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab089282-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,099.90 |
Recombinant; Rabbit anti Human Apolipoprotein A I Antibody; WB; Unconjugated
| Product Name | Apolipoprotein A I Mouse mAb - Primary antibody, specific to Apolipoprotein A I, Mouse IgG2b |
|---|---|
| Synonyms | ApoA-I | Apo-AI | Apolipoprotein A1 | Apo-AI antibody | ApoA Iantibody | ApoA-I antibody | APOA1 antibody | APOA1_HUMAN antibody | Apolipoprotein A-I(1-242) antibody | Apolipoprotein A1 antibody | Apolipoprotein AI antibody | Apolipoprotein of high densit |
| Specifications & Purity | ExactAb™, Validated, High performance, Recombinant, Lot by Lot |
| Biochemical and Physiological Mechanisms | Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activat |
| Host species | Rabbit |
| Specificity | Apolipoprotein A I |
| Immunogen | A synthetic peptide derived from human Apolipoprotein A I (AA 13-50). |
| Positive Control | WB: Huamn serum lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description | Rabbit anti Human Apolipoprotein A I Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Apolipoprotein A I Antibody (Ab089282) - Western Blot
All lanes: Recombinant Apolipoprotein A I Antibody (Ab089282) at 1/1000 dilution
Samples: Lysates at 20 µL (1/100 dilution) per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 25 kDa
Exposure time: 26.9 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/5000 "> 1/1000 - 1/5000 |
Enter Lot Number to search for COA:
| 1. Furuhashi M, Tuncman G, Görgün CZ, Makowski L, Atsumi G, Vaillancourt E, Kono K, Babaev VR, Fazio S, Linton MF et al.. (2007) Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2.. Nature, 447 (7147): (959-65). [PMID:17554340] [10.1021/op500134e] |
| 2. Iio A, Ohguchi K, Iinuma M, Nozawa Y, Ito M.. (2012) Hesperetin upregulates ABCA1 expression and promotes cholesterol efflux from THP-1 macrophages.. J Nat Prod, 75 (4): (563-566). [PMID:22429094] [10.1021/np200696r] |
| 3. Baker, H N HN, Gotto, A M AM and Jackson, R L RL.. (1975) The primary structure of human plasma high density apolipoprotein glutamine I (ApoA-I). II. The amino acid sequence and alignment of cyanogen bromide fragments IV, III, and I.. The Journal of biological chemistry, (10): [PMID:164450] |
| 4. Brewer, H B HB and 5 more authors.. (1978) The amino acid sequence of human APOA-I, an apolipoprotein isolated from high density lipoproteins.. Biochemical and biophysical research communications, (14): [PMID:204308] |
| 5. Soutar, A K AK and 9 more authors.. (1992) Apolipoprotein AI mutation Arg-60 causes autosomal dominant amyloidosis.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:1502149] |
| 6. Funke, H H and 5 more authors.. (1991) A frameshift mutation in the human apolipoprotein A-I gene causes high density lipoprotein deficiency, partial lecithin: cholesterol-acyltransferase deficiency, and corneal opacities.. The Journal of clinical investigation, [PMID:1898657] |
| 7. Matsunaga, T T and 9 more authors.. (1991) Apolipoprotein A-I deficiency due to a codon 84 nonsense mutation of the apolipoprotein A-I gene.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1901417] |
| 8. Takada, Y Y and 5 more authors.. (1990) Isolation and characterization of human apolipoprotein A-I Fukuoka (110 Glu----Lys). A novel apolipoprotein variant.. Biochimica et biophysica acta, (2): [PMID:2107878] |
| 9. Ladias, J A JA, Kwiterovich, P O PO, Smith, H H HH, Karathanasis, S K SK and Antonarakis, S E SE.. (1990) Apolipoprotein A1 Baltimore (Arg10----Leu), a new ApoA1 variant.. Human genetics, [PMID:2108924] |
| 10. von Eckardstein, A A and 5 more authors.. (1990) Structural analysis of human apolipoprotein A-I variants. Amino acid substitutions are nonrandomly distributed throughout the apolipoprotein A-I primary structure.. The Journal of biological chemistry, (25): [PMID:2111322] |
| 11. Nichols, W C WC, Gregg, R E RE, Brewer, H B HB and Benson, M D MD.. (1990) A mutation in apolipoprotein A-I in the Iowa type of familial amyloidotic polyneuropathy.. Genomics, [PMID:2123470] |
| 12. Manjunath, P P and 5 more authors.. (1989) Apolipoprotein A-I binds to a family of bovine seminal plasma proteins.. The Journal of biological chemistry, (5): [PMID:2506184] |
| 13. von Eckardstein, A A and 5 more authors.. (1989) Apolipoprotein A-I variants. Naturally occurring substitutions of proline residues affect plasma concentration of apolipoprotein A-I.. The Journal of clinical investigation, [PMID:2512329] |
| 14. Moguilevsky, N N and 9 more authors.. () Production of human recombinant proapolipoprotein A-I in Escherichia coli: purification and biochemical characterization.. DNA (Mary Ann Liebert, Inc.), [PMID:2673706] |
| 15. Law, S W SW and Brewer, H B HB.. (1985) Tangier disease. The complete mRNA sequence encoding for preproapo-A-I.. The Journal of biological chemistry, (15): [PMID:2995392] |
| 16. Hoeg, J M JM, Meng, M S MS, Ronan, R R, Fairwell, T T and Brewer, H B HB.. (1986) Human apolipoprotein A-I. Post-translational modification by fatty acid acylation.. The Journal of biological chemistry, (25): [PMID:3005308] |
| 17. Yui, Y Y and 5 more authors.. (1988) Serum prostacyclin stabilizing factor is identical to apolipoprotein A-I (Apo A-I). A novel function of Apo A-I.. The Journal of clinical investigation, [PMID:3047170] |
| 18. Prioli, R P RP, Ordovas, J M JM, Rosenberg, I I, Schaefer, E J EJ and Pereira, M E ME.. (1987) Similarity of cruzin, an inhibitor of Trypanosoma cruzi neuraminidase, to high-density lipoprotein.. Science (New York, N.Y.), (4): [PMID:3120314] |
| 19. Makrides, S C SC and 5 more authors.. (1988) Sequence and expression of Tangier apoA-I gene.. European journal of biochemistry, (15): [PMID:3129297] |
| 20. Nichols, W C WC, Dwulet, F E FE, Liepnieks, J J and Benson, M D MD.. (1988) Variant apolipoprotein AI as a major constituent of a human hereditary amyloid.. Biochemical and biophysical research communications, (31): [PMID:3142462] |
| 21. Law, S W SW and Brewer, H B HB.. (1984) Nucleotide sequence and the encoded amino acids of human apolipoprotein A-I mRNA.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6198645] |
| 22. Seilhamer, J J JJ, Protter, A A AA, Frossard, P P and Levy-Wilson, B B.. (1984) Isolation and DNA sequence of full-length cDNA and of the entire gene for human apolipoprotein AI--discovery of a new genetic polymorphism in the apo AI gene.. DNA (Mary Ann Liebert, Inc.), [PMID:6207999] |
| 23. Breslow, J L JL and 7 more authors.. (1982) Isolation and characterization of cDNA clones for human apolipoprotein A-I.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6294659] |
| 24. Cheung, P P and Chan, L L.. (1983) Nucleotide sequence of cloned cDNA of human apolipoprotein A-I.. Nucleic acids research, (11): [PMID:6304641] |
| 25. Sharpe, C R CR and 5 more authors.. (1984) Human apolipoproteins AI, AII, CII and CIII. cDNA sequences and mRNA abundance.. Nucleic acids research, (11): [PMID:6328445] |
| 26. Weisgraber, K H KH and 5 more authors.. (1983) Apolipoprotein A-IMilano. Detection of normal A-I in affected subjects and evidence for a cysteine for arginine substitution in the variant A-I.. The Journal of biological chemistry, (25): [PMID:6401735] |
| 27. Zannis, V I VI, Karathanasis, S K SK, Keutmann, H T HT, Goldberger, G G and Breslow, J L JL.. (1983) Intracellular and extracellular processing of human apolipoprotein A-I: secreted apolipoprotein A-I isoprotein 2 is a propeptide.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6405383] |
| 28. Shoulders, C C CC, Kornblihtt, A R AR, Munro, B S BS and Baralle, F E FE.. (1983) Gene structure of human apolipoprotein A1.. Nucleic acids research, (11): [PMID:6406984] |
| 29. Brewer, H B HB and 6 more authors.. (1983) Human plasma proapoA-I: isolation and amino-terminal sequence.. Biochemical and biophysical research communications, (15): [PMID:6409108] |
| 30. Schmitz, G G, Assmann, G G, Rall, S C SC and Mahley, R W RW.. (1983) Tangier disease: defective recombination of a specific Tangier apolipoprotein A-I isoform (pro-apo A-i) with high density lipoproteins.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6412234] |
| 31. Karathanasis, S K SK, Zannis, V I VI and Breslow, J L JL.. (1983) Isolation and characterization of the human apolipoprotein A-I gene.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6413973] |
| 32. Rall, S C SC and 9 more authors.. (1984) Abnormal lecithin:cholesterol acyltransferase activation by a human apolipoprotein A-I variant in which a single lysine residue is deleted.. The Journal of biological chemistry, (25): [PMID:6432779] |
| 33. Utermann, G G and 6 more authors.. (1984) Apolipoprotein A-IGiessen (Pro143----Arg). A mutant that is defective in activating lecithin:cholesterol acyltransferase.. European journal of biochemistry, (15): [PMID:6489332] |
| 34. Schaefer, E J EJ, Heaton, W H WH, Wetzel, M G MG and Brewer, H B HB.. () Plasma apolipoprotein A-1 absence associated with a marked reduction of high density lipoproteins and premature coronary artery disease.. Arteriosclerosis (Dallas, Tex.), [PMID:6800349] |
| 35. Corbett, J M JM, Wheeler, C H CH, Baker, C S CS, Yacoub, M H MH and Dunn, M J MJ.. (1994) The human myocardial two-dimensional gel protein database: update 1994.. Electrophoresis, [PMID:7895732] |
| 36. Araki, K K and 6 more authors.. (1994) Characterization of two new human apolipoprotein A-I variants: apolipoprotein A-I Tsushima (Trp-108-->Arg) and A-I Hita (Ala-95-->Asp).. Biochimica et biophysica acta, (6): [PMID:7918609] |
| 37. Römling, R R and 6 more authors.. (1994) A nonsense mutation in the apolipoprotein A-I gene is associated with high-density lipoprotein deficiency and periorbital xanthelasmas.. Arteriosclerosis and thrombosis : a journal of vascular biology, [PMID:7981179] |
| 38. Vigushin, D M DM and 9 more authors.. (1994) Familial nephropathic systemic amyloidosis caused by apolipoprotein AI variant Arg26.. The Quarterly journal of medicine, [PMID:8208902] |
| 39. Nakata, K K and 6 more authors.. (1993) Autosomal dominant hypoalphalipoproteinemia due to a completely defective apolipoprotein A-I gene.. Biochemical and biophysical research communications, (29): [PMID:8240372] |
| 40. Ng, D S DS, Leiter, L A LA, Vezina, C C, Connelly, P W PW and Hegele, R A RA.. (1994) Apolipoprotein A-I Q[-2]X causing isolated apolipoprotein A-I deficiency in a family with analphalipoproteinemia.. The Journal of clinical investigation, [PMID:8282791] |
| 41. Wang, G G, Treleaven, W D WD and Cushley, R J RJ.. (1996) Conformation of human serum apolipoprotein A-I(166-185) in the presence of sodium dodecyl sulfate or dodecylphosphocholine by 1H-NMR and CD. Evidence for specific peptide-SDS interactions.. Biochimica et biophysica acta, (11): [PMID:8664326] |
| 42. Borhani, D W DW, Rogers, D P DP, Engler, J A JA and Brouillette, C G CG.. (1997) Crystal structure of truncated human apolipoprotein A-I suggests a lipid-bound conformation.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:9356442] |
| 43. Huang, W W and 9 more authors.. (1998) A novel homozygous missense mutation in the apo A-I gene with apo A-I deficiency.. Arteriosclerosis, thrombosis, and vascular biology, [PMID:9514407] |
| 44. Hamidi Asl, K K, Liepnieks, J J JJ, Nakamura, M M, Parker, F F and Benson, M D MD.. (1999) A novel apolipoprotein A-1 variant, Arg173Pro, associated with cardiac and cutaneous amyloidosis.. Biochemical and biophysical research communications, (13): [PMID:10198255] |
| 45. Obici, L L and 11 more authors.. (1999) The new apolipoprotein A-I variant leu(174) --> Ser causes hereditary cardiac amyloidosis, and the amyloid fibrils are constituted by the 93-residue N-terminal polypeptide.. The American journal of pathology, [PMID:10487826] |
| 46. Ritter, Mirko M and 10 more authors.. (2002) Cloning and characterization of a novel apolipoprotein A-I binding protein, AI-BP, secreted by cells of the kidney proximal tubules in response to HDL or ApoA-I.. Genomics, [PMID:11991719] |
| 47. Lachmann, Helen J HJ and 7 more authors.. (2002) Misdiagnosis of hereditary amyloidosis as AL (primary) amyloidosis.. The New England journal of medicine, (6): [PMID:12050338] |
| 48. Niederkofler, Eric E EE, Tubbs, Kemmons A KA, Kiernan, Urban A UA, Nedelkov, Dobrin D and Nelson, Randall W RW.. (2003) Novel mass spectrometric immunoassays for the rapid structural characterization of plasma apolipoproteins.. Journal of lipid research, [PMID:12562854] |
| 49. Pankhurst, Greg G and 6 more authors.. (2003) Characterization of specifically oxidized apolipoproteins in mildly oxidized high density lipoprotein.. Journal of lipid research, [PMID:12576517] |
| 50. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 51. Bin, Liang-Hua LH, Nielson, Larry D LD, Liu, Xinqi X, Mason, Robert J RJ and Shu, Hong-Bing HB.. (2003) Identification of uteroglobin-related protein 1 and macrophage scavenger receptor with collagenous structure as a lung-specific ligand-receptor pair.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:12847263] |
| 52. Morabia, Alfredo A and 7 more authors.. (2003) Association of extreme blood lipid profile phenotypic variation with 11 reverse cholesterol transport genes and 10 non-genetic cardiovascular disease risk factors.. Human molecular genetics, (1): [PMID:12966036] |
| 53. Fullerton, Stephanie M SM and 11 more authors.. (2004) The effects of scale: variation in the APOA1/C3/A4/A5 gene cluster.. Human genetics, [PMID:15108119] |
| 54. Hunter, Michael M and 7 more authors.. (2005) NDRG1 interacts with APO A-I and A-II and is a functional candidate for the HDL-C QTL on 8q24.. Biochemical and biophysical research communications, (15): [PMID:15922294] |
| 55. Mooradian, Arshag D AD, Haas, Michael J MJ and Wong, Norman C W NC.. (2006) The effect of select nutrients on serum high-density lipoprotein cholesterol and apolipoprotein A-I levels.. Endocrine reviews, [PMID:16243964] |
| 56. Fang, Longhou L and 12 more authors.. (2013) Control of angiogenesis by AIBP-mediated cholesterol efflux.. Nature, (6): [PMID:23719382] |
| 57. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 58. Petrlova, Jitka J and 7 more authors.. (2014) Secondary structure changes in ApoA-I Milano (R173C) are not accompanied by a decrease in protein stability or solubility.. PloS one, [PMID:24755625] |
| 59. Anthanont, Pimjai P, Asztalos, Bela F BF, Polisecki, Eliana E, Zachariah, Benoy B and Schaefer, Ernst J EJ.. () Case report: A novel apolipoprotein A-I missense mutation apoA-I (Arg149Ser)Boston associated with decreased lecithin-cholesterol acyltransferase activation and cellular cholesterol efflux.. Journal of clinical lipidology, [PMID:26073399] |
| 60. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
