Apolipoprotein B Mouse mAb - Primary antibody, specific to Apolipoprotein B, Mouse IgG2b

    Application:
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG2b
  • Clone Number: E6
  • Conjugation: Unconjugated
Item Number
Ab089308
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab089308-10μl
10μl
In stock
$69.90
Ab089308-50μl
50μl
In stock
$199.90
Ab089308-100μl
100μl
In stock
$319.90
Ab089308-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (E6); Mouse anti Human Apolipoprotein B Antibody; WB; Unconjugated

Basic Description

Product NameApolipoprotein B Mouse mAb - Primary antibody, specific to Apolipoprotein B, Mouse IgG2b
SynonymsApo B-100 | Apo B 100 antibody | Apo B antibody | Apo B-100 antibody | Apo B-48 antibody | Apo B100 antibody | Apo B48 antibody | ApoB 100antibody | ApoB 48 antibody | APOB antibody | APOB_HUMAN antibody | Apolipoprotein B (including Ag(x) antigen) antibo
Specifications & PurityExactAb™, Validated, Carrier Free, Lot by Lot
Host speciesMouse
SpecificityApolipoprotein B
ImmunogenNative Apolipoprotein B (APOB).
Positive ControlWB: Human Serum lysates.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Mouse anti Human Apolipoprotein B Antibody, Monoclonal (E6), could be used for WB and so on.
Application:
WB: 0.01-3 µg/mL
Protein Function:
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Product Properties

IsotypeMouse IgG2b
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified + Protein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Apolipoprotein B Mouse mAb (Ab089308) - Western Blot
All lanes: Apolipoprotein B Mouse mAb (Ab089308) at 1/1000 dilution
Samples: Lysates at 20 µL per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 260-500 kDa
Observed band size: 464 kDa
Exposure time: 20.6 s

Associated Targets(Human)

APOB Tchem Apolipoprotein B-100 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB

0.01-3 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404356Certificate of AnalysisApr 10, 2024 Ab089308
ZJ24F0404355Certificate of AnalysisApr 10, 2024 Ab089308
ZJ24F0404354Certificate of AnalysisApr 10, 2024 Ab089308

Related Documents

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861] [10.1021/op500134e]
2. Navajas, M M and 7 more authors..  (1990)  Detection by denaturing gradient gel electrophoresis of a new polymorphism in the apolipoprotein B gene..  Human genetics,      [PMID:1979313]
3. Yang, C Y CY and 5 more authors..  (1990)  Isolation and characterization of sulfhydryl and disulfide peptides of human apolipoprotein B-100..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2115173]
4. Huang, L S LS, Gavish, D D and Breslow, J L JL..  (1990)  Sequence polymorphism in the human apoB gene at position 8344..  Nucleic acids research,    (11):   [PMID:2216805]
5. Hospattankar, A V AV, Higuchi, K K, Law, S W SW, Meglin, N N and Brewer, H B HB..  (1987)  Identification of a novel in-frame translational stop codon in human intestine apoB mRNA..  Biochemical and biophysical research communications,    (14):   [PMID:2445342]
6. Higuchi, K K and 5 more authors..  (1988)  Human apolipoprotein B (apoB) mRNA: identification of two distinct apoB mRNAs, an mRNA with the apoB-100 sequence and an apoB mRNA containing a premature in-frame translational stop codon, in both liver and intestine..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2450346]
7. Soria, L F LF and 5 more authors..  (1989)  Association between a specific apolipoprotein B mutation and familial defective apolipoprotein B-100..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2563166]
8. Huang, L S LS, Ripps, M E ME, Korman, S H SH, Deckelbaum, R J RJ and Breslow, J L JL..  (1989)  Hypobetalipoproteinemia due to an apolipoprotein B gene exon 21 deletion derived by Alu-Alu recombination..  The Journal of biological chemistry,    (5):   [PMID:2567736]
9. Carlsson, P P, Darnfors, C C, Olofsson, S O SO and Bjursell, G G..  (1986)  Analysis of the human apolipoprotein B gene; complete structure of the B-74 region..  Gene,      [PMID:2883086]
10. Wei, C F CF and 8 more authors..  (1985)  Molecular cloning and expression of partial cDNAs and deduced amino acid sequence of a carboxyl-terminal fragment of human apolipoprotein B-100..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2932736]
11. Knott, T J TJ and 9 more authors..  (1985)  Human apolipoprotein B: structure of carboxyl-terminal domains, sites of gene expression, and chromosomal localization..  Science (New York, N.Y.),    (4):   [PMID:2994225]
12. Law, S W SW and 6 more authors..  (1985)  Human apolipoprotein B-100: cloning, analysis of liver mRNA, and assignment of the gene to chromosome 2..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3001697]
13. Pfitzner, R R, Wagener, R R and Stoffel, W W..  (1986)  Isolation, expression and characterization of a human apolipoprotein B 100-specific cDNA clone..  Biological chemistry Hoppe-Seyler,      [PMID:3024665]
14. Cladaras, C C, Hadzopoulou-Cladaras, M M, Nolte, R T RT, Atkinson, D D and Zannis, V I VI..  (1986)  The complete sequence and structural analysis of human apolipoprotein B-100: relationship between apoB-100 and apoB-48 forms..  The EMBO journal,    (20):   [PMID:3030729]
15. Dashti, N N, Lee, D M DM and Mok, T T..  (1986)  Apolipoprotein B is a calcium binding protein..  Biochemical and biophysical research communications,    (29):   [PMID:3087360]
16. Yang, C Y CY and 9 more authors..  (1986)  Sequence, structure, receptor-binding domains and internal repeats of human apolipoprotein B-100..  Nature,      [PMID:3095664]
17. Hardman, D A DA, Protter, A A AA, Schilling, J W JW and Kane, J P JP..  (1987)  Carboxyl terminal analysis of human B-48 protein confirms the novel mechanism proposed for chain termination..  Biochemical and biophysical research communications,    (31):   [PMID:3426612]
18. Protter, A A AA and 8 more authors..  (1986)  Analysis of cDNA clones encoding the entire B-26 region of human apolipoprotein B..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3461454]
19. Law, S W SW and 6 more authors..  (1986)  Human liver apolipoprotein B-100 cDNA: complete nucleic acid and derived amino acid sequence..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3464946]
20. Protter, A A AA and 8 more authors..  (1986)  Isolation of a cDNA clone encoding the amino-terminal region of human apolipoprotein B..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3513177]
21. Powell, L M LM and 5 more authors..  (1987)  A novel form of tissue-specific RNA processing produces apolipoprotein-B48 in intestine..  Cell,    (11):   [PMID:3621347]
22. Ludwig, E H EH and 9 more authors..  (1987)  DNA sequence of the human apolipoprotein B gene..  DNA (Mary Ann Liebert, Inc.),      [PMID:3652907]
23. Chen, S H SH and 9 more authors..  (1987)  Apolipoprotein B-48 is the product of a messenger RNA with an organ-specific in-frame stop codon..  Science (New York, N.Y.),    (16):   [PMID:3659919]
24. Hardman, D A DA and 9 more authors..  (1987)  Structural comparison of human apolipoproteins B-48 and B-100..  Biochemistry,    (25):   [PMID:3676265]
25. Chen, S H SH and 7 more authors..  (1986)  The complete cDNA and amino acid sequence of human apolipoprotein B-100..  The Journal of biological chemistry,    (5):   [PMID:3759943]
26. Knott, T J TJ and 9 more authors..  (1986)  Complete cDNA and derived protein sequence of human apolipoprotein B-100..  Nucleic acids research,    (25):   [PMID:3763409]
27. Knott, T J TJ and 9 more authors..  (1986)  Complete protein sequence and identification of structural domains of human apolipoprotein B..  Nature,      [PMID:3773997]
28. Carlsson, P P and 5 more authors..  (1985)  Molecular cloning of human apolipoprotein B cDNA..  Nucleic acids research,    (20):   [PMID:3841204]
29. Shoulders, C C CC and 6 more authors..  (1985)  Molecular cloning of human LDL apolipoprotein B cDNA. Evidence for more than one gene per haploid genome..  Atherosclerosis,      [PMID:3841481]
30. Deeb, S S SS, Motulsky, A G AG and Albers, J J JJ..  (1985)  A partial cDNA clone for human apolipoprotein B..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3860836]
31. Mehrabian, M M and 9 more authors..  (1985)  Human apolipoprotein B: identification of cDNA clones and characterization of mRNA..  Nucleic acids research,    (11):   [PMID:3903660]
32. LeBoeuf, R C RC and 5 more authors..  (1984)  Human apolipoprotein B: partial amino acid sequence..  FEBS letters,    (7):   [PMID:6373369]
33. Pullinger, C R CR and 9 more authors..  (1995)  Familial ligand-defective apolipoprotein B. Identification of a new mutation that decreases LDL receptor binding affinity..  The Journal of clinical investigation,      [PMID:7883971]
34. Poirier, O O and 9 more authors..  (1996)  Detection of new variants in the apolipoprotein B (Apo B) gene by PCR-SSCP..  Human mutation,      [PMID:8889592]
35. Rabès, J P JP and 8 more authors..  (1997)  Familial ligand-defective apolipoprotein B-100: simultaneous detection of the ARG3500-->GLN and ARG3531-->CYS mutations in a French population..  Human mutation,      [PMID:9259199]
36. Leren, T P TP, Bakken, K S KS, Hoel, V V, Hjermann, I I and Berg, K K..  (1998)  Screening for mutations of the apolipoprotein B gene causing hypocholesterolemia..  Human genetics,      [PMID:9490296]
37. Zhao, Y Y, McCabe, J B JB, Vance, J J and Berthiaume, L G LG..  (2000)  Palmitoylation of apolipoprotein B is required for proper intracellular sorting and transport of cholesteroyl esters and triglycerides..  Molecular biology of the cell,      [PMID:10679026]
38. Burnett, John R JR and 8 more authors..  (2003)  A novel nontruncating APOB gene mutation, R463W, causes familial hypobetalipoproteinemia..  The Journal of biological chemistry,    (11):   [PMID:12551903]
39. Lancellotti, Sandra S and 7 more authors..  (2004)  Hypobetalipoproteinemia with an apparently recessive inheritance due to a "de novo" mutation of apolipoprotein B..  Biochimica et biophysica acta,    (20):   [PMID:14732481]
40. Bunkenborg, Jakob J, Pilch, Bartosz J BJ, Podtelejnikov, Alexandre V AV and Wiśniewski, Jacek R JR..  (2004)  Screening for N-glycosylated proteins by liquid chromatography mass spectrometry..  Proteomics,      [PMID:14760718]
41. Hillier, Ladeana W LW and 121 more authors..  (2005)  Generation and annotation of the DNA sequences of human chromosomes 2 and 4..  Nature,    (7):   [PMID:15815621]
42. Liu, Tao T and 6 more authors..  ()  Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry..  Journal of proteome research,      [PMID:16335952]
43. Leong, Wai Fook WF and Chow, Vincent T K VT..  (2006)  Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection..  Cellular microbiology,      [PMID:16548883]
44. Sjöblom, Tobias T and 28 more authors..  (2006)  The consensus coding sequences of human breast and colorectal cancers..  Science (New York, N.Y.),    (13):   [PMID:16959974]
45. Teslovich, Tanya M TM and 208 more authors..  (2010)  Biological, clinical and population relevance of 95 loci for blood lipids..  Nature,    (5):   [PMID:20686565]
46. van der Graaf, Anouk A and 10 more authors..  (2011)  Molecular basis of autosomal dominant hypercholesterolemia: assessment in a large cohort of hypercholesterolemic children..  Circulation,    (22):   [PMID:21382890]
47. Gangloff, Anne A and 5 more authors..  ()  A novel mutation of apolipoprotein B in a French Canadian family with homozygous hypobetalipoproteinemia..  Journal of clinical lipidology,      [PMID:21981844]
48. Huijgen, Roeland R and 7 more authors..  (2012)  Genetic variation in APOB, PCSK9, and ANGPTL3 in carriers of pathogenic autosomal dominant hypercholesterolemic mutations with unexpected low LDL-Cl Levels..  Human mutation,      [PMID:22095935]
49. Sun, Hua H and 5 more authors..  (2012)  Proprotein convertase subtilisin/kexin type 9 interacts with apolipoprotein B and prevents its intracellular degradation, irrespective of the low-density lipoprotein receptor..  Arteriosclerosis, thrombosis, and vascular biology,      [PMID:22580899]
50. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,    (16):   [PMID:24275569]
51. Tagliabracci, Vincent S VS and 14 more authors..  (2015)  A Single Kinase Generates the Majority of the Secreted Phosphoproteome..  Cell,    (18):   [PMID:26091039]
52. Walsh, Meghan T MT and 8 more authors..  (2015)  Novel Abetalipoproteinemia Missense Mutation Highlights the Importance of the N-Terminal β-Barrel in Microsomal Triglyceride Transfer Protein Function..  Circulation. Cardiovascular genetics,      [PMID:26224785]
53. Miller, Sharon A SA and 6 more authors..  ()  Novel APOB missense variants, A224T and V925L, in a black South African woman with marked hypocholesterolemia..  Journal of clinical lipidology,      [PMID:27206948]
54. Zhang, Jing J and 6 more authors..  (2017)  AUP1 (Ancient Ubiquitous Protein 1) Is a Key Determinant of Hepatic Very-Low-Density Lipoprotein Assembly and Secretion..  Arteriosclerosis, thrombosis, and vascular biology,      [PMID:28183703]
55. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

Solution Calculators