Basic Description

Product Name	ARFGAP1 Antibody - Primary antibody, specific to ARFGAP1, Rabbit IgG
Specifications & Purity	ExactAb™, Validated, Carrier Free, 0.3 mg/mL
Host species	Rabbit
Specificity	ARFGAP1
Immunogen	Fusion protein of human ARFGAP1 (AA 259-406)
Positive Control	WB: HEK293, MOLT-4, Hep G2, HeLa, MCF7, NIH/3T3 and C6 cell lysates.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Rabbit anti Human ARFGAP1 Antibody, Polyclonal, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and RNP24. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.3 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

ARFGAP1 Antibody (Ab089481) - Western Blot
All lanes: ARFGAP1 Antibody (Ab089481) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 45, 47 kDa
Exposure time: 6.2 s

Associated Targets

ARFGAP1 Tbio ADP-ribosylation factor GTPase-activating protein 1 0 Activities

Discover Target: ARFGAP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505876	Certificate of Analysis	May 21, 2024	Ab089481
ZJ24F0505877	Certificate of Analysis	May 21, 2024	Ab089481

FAQs and Articles

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Guidelines of Primary Antibodies for Western Blotting

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Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

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SKU	Size	Availability	Price
Ab089481-10μl	10μl	In stock	$79.90
Ab089481-50μl	50μl	In stock	$199.90
Ab089481-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab089481-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,899.90

ARFGAP1 Antibody - Primary antibody, specific to ARFGAP1, Rabbit IgG