ARIH2/TRIAD1 Antibody - Primary antibody, specific to ARIH2, Rabbit IgG
Synonyms
ARI-2 | EC 2.3.2.31 | Protein ariadne-2 homolog | RING-type E3 ubiquitin transferase ARIH2 | Triad1 protein | HT005 antibody | all trans retinoic acid inducible RING finger antibody | Ari 2 antibody | Ari-2 antibody | ARI2 antibody | ARI2_HUMAN antibody |
Specifications & Purity
ExactAb™, Validated, Carrier Free, 0.6 mg/mL
Host species
Rabbit
Specificity
ARIH2
Immunogen
A synthetic peptide derived from human ARIH2/TRIAD1 (AA 321-349)
Positive Control
WB: HeLa and K562 cell lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Product Description
Rabbit anti Human ARIH2/TRIAD1 Antibody, Polyclonal, could be used for WB and so on. Application WB: 1/500-1/2000 Protein Function E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.6 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
ARIH2/TRIAD1 Antibody (Ab089598) - Western Blot All lanes: ARIH2/TRIAD1 Antibody (Ab089598) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa Observed band size: 57 kDa Exposure time: 30.1 s
1.van der Reijden, B A BA, Erpelinck-Verschueren, C A CA, Löwenberg, B B and Jansen, J H JH.. (1999) TRIADs: a new class of proteins with a novel cysteine-rich signature.. Protein science : a publication of the Protein Society, [PMID:10422847]
2.Aguilera, M M, Oliveros, M M, Martínez-Padrón, M M, Barbas, J A JA and Ferrús, A A.. (2000) Ariadne-1: a vital Drosophila gene is required in development and defines a new conserved family of ring-finger proteins.. Genetics, [PMID:10880484]
3.Hu, R M RM and 28 more authors.. (2000) Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.. Proceedings of the National Academy of Sciences of the United States of America, (15):[PMID:10931946]
4.Marteijn, Jurgen A F JA and 8 more authors.. (2005) The E3 ubiquitin-protein ligase Triad1 inhibits clonogenic growth of primary myeloid progenitor cells.. Blood, (15):[PMID:16118314]
5.Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25):[PMID:17525332]
6.Marteijn, Jurgen A F JA and 7 more authors.. (2007) Gfi1 ubiquitination and proteasomal degradation is inhibited by the ubiquitin ligase Triad1.. Blood, (1):[PMID:17646546]
7.Marteijn, J A JA and 11 more authors.. (2009) The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains.. Leukemia, [PMID:19340006]
8.Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4):[PMID:23186163]
9.Kelsall, Ian R IR and 9 more authors.. (2013) TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes.. The EMBO journal, (30):[PMID:24076655]
10.Kelsall, Ian R and 5 more authors.. (2019) Coupled monoubiquitylation of the co-E3 ligase DCNL1 by Ariadne-RBR E3 ubiquitin ligases promotes cullin-RING ligase complex remodeling.. The Journal of biological chemistry, (22):[PMID:30587576]
11.Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]
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