ARIH2/TRIAD1 Antibody - Primary antibody, specific to ARIH2, Rabbit IgG

    Application:
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab089598
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab089598-10μl
10μl
In stock
$59.90
Ab089598-50μl
50μl
In stock
$189.90
Ab089598-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$279.90
Ab089598-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,789.90

pAb; Rabbit anti Human ARIH2/TRIAD1 Antibody; WB; Unconjugated

Basic Description

Product NameARIH2/TRIAD1 Antibody - Primary antibody, specific to ARIH2, Rabbit IgG
SynonymsARI-2 | EC 2.3.2.31 | Protein ariadne-2 homolog | RING-type E3 ubiquitin transferase ARIH2 | Triad1 protein | HT005 antibody | all trans retinoic acid inducible RING finger antibody | Ari 2 antibody | Ari-2 antibody | ARI2 antibody | ARI2_HUMAN antibody |
Specifications & PurityExactAb™, Validated, Carrier Free, 0.6 mg/mL
Host speciesRabbit
SpecificityARIH2
ImmunogenA synthetic peptide derived from human ARIH2/TRIAD1 (AA 321-349)
Positive ControlWB: HeLa and K562 cell lysates.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human ARIH2/TRIAD1 Antibody, Polyclonal, could be used for WB and so on.
Application
WB: 1/500-1/2000
Protein Function
E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.6 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

ARIH2/TRIAD1 Antibody (Ab089598) - Western Blot
All lanes: ARIH2/TRIAD1 Antibody (Ab089598) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 57 kDa
Exposure time: 30.1 s

Associated Targets(Human)

ARIH2 Tbio E3 ubiquitin-protein ligase ARIH2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/2000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303795Certificate of AnalysisMar 27, 2024 Ab089598
ZJ24F0303794Certificate of AnalysisMar 27, 2024 Ab089598

Related Documents

References

1. van der Reijden, B A BA, Erpelinck-Verschueren, C A CA, Löwenberg, B B and Jansen, J H JH..  (1999)  TRIADs: a new class of proteins with a novel cysteine-rich signature..  Protein science : a publication of the Protein Society,      [PMID:10422847]
2. Aguilera, M M, Oliveros, M M, Martínez-Padrón, M M, Barbas, J A JA and Ferrús, A A..  (2000)  Ariadne-1: a vital Drosophila gene is required in development and defines a new conserved family of ring-finger proteins..  Genetics,      [PMID:10880484]
3. Hu, R M RM and 28 more authors..  (2000)  Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning..  Proceedings of the National Academy of Sciences of the United States of America,    (15):   [PMID:10931946]
4. Marteijn, Jurgen A F JA and 8 more authors..  (2005)  The E3 ubiquitin-protein ligase Triad1 inhibits clonogenic growth of primary myeloid progenitor cells..  Blood,    (15):   [PMID:16118314]
5. Matsuoka, Shuhei S and 12 more authors..  (2007)  ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage..  Science (New York, N.Y.),    (25):   [PMID:17525332]
6. Marteijn, Jurgen A F JA and 7 more authors..  (2007)  Gfi1 ubiquitination and proteasomal degradation is inhibited by the ubiquitin ligase Triad1..  Blood,    (1):   [PMID:17646546]
7. Marteijn, J A JA and 11 more authors..  (2009)  The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains..  Leukemia,      [PMID:19340006]
8. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,    (4):   [PMID:23186163]
9. Kelsall, Ian R IR and 9 more authors..  (2013)  TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes..  The EMBO journal,    (30):   [PMID:24076655]
10. Kelsall, Ian R and 5 more authors..  (2019)  Coupled monoubiquitylation of the co-E3 ligase DCNL1 by Ariadne-RBR E3 ubiquitin ligases promotes cullin-RING ligase complex remodeling..  The Journal of biological chemistry,    (22):   [PMID:30587576]
11. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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