pAb; Rabbit anti Human ATF-4 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab089952-10μl | 10μl | In stock | $66.90 | |
| Ab089952-50μl | 50μl | In stock | $199.90 | |
| Ab089952-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab089952-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
pAb; Rabbit anti Human ATF-4 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | ATF-4 Antibody - Primary antibody, specific to ATF4, Rabbit IgG |
|---|---|
| Synonyms | activating transcription factor 4 (tax-responsive enhancer element B67); Activating transcription factor 4; ATF4; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2DNA-binding protein TAXREB67; cyclic AMP-dependen |
| Specifications & Purity | ExactAb™, Validated, 1.5 mg/mL |
| Host species | Rabbit |
| Specificity | ATF4 |
| Immunogen | A synthetic peptide derived from human ATF4 (AA 1-220). |
| Positive Control | WB: HeLa and HEK293 cell lysates. IHC: Mouse kidney tissue. ICC/IF: 293 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Rabbit anti Human ATF-4 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
ATF-4 Antibody (Ab089952) - Western Blot
All lanes: ATF-4 Antibody (Ab089952) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 54 kDa
Exposure time: 20.0 s
ATF-4 Antibody (Ab089952) - IF/ICC
Immunofluorescence analysis of ATF4 in 293 using ATF-4 Antibody (Ab089952).
ATF-4 Antibody (Ab089952) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse kidney using ATF-4 Antibody (Ab089952). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303189 | Certificate of Analysis | Mar 13, 2024 | Ab089952 |
| ZJ24F0303188 | Certificate of Analysis | Mar 13, 2024 | Ab089952 |
| 1. Karpinski, B A BA, Morle, G D GD, Huggenvik, J J, Uhler, M D MD and Leiden, J M JM.. (1992) Molecular cloning of human CREB-2: an ATF/CREB transcription factor that can negatively regulate transcription from the cAMP response element.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1534408] |
| 2. Tsujimoto, A A, Nyunoya, H H, Morita, T T, Sato, T T and Shimotohno, K K.. (1991) Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I.. Journal of virology, [PMID:1847461] |
| 3. Hai, T W TW, Liu, F F, Coukos, W J WJ and Green, M R MR.. (1989) Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.. Genes & development, [PMID:2516827] |
| 4. Dunham, I I and 24 more authors.. (1999) The DNA sequence of human chromosome 22.. Nature, (2): [PMID:10591208] |
| 5. Podust, L M LM, Krezel, A M AM and Kim, Y Y.. (2001) Crystal structure of the CCAAT box/enhancer-binding protein beta activating transcription factor-4 basic leucine zipper heterodimer in the absence of DNA.. The Journal of biological chemistry, (5): [PMID:11018027] |
| 6. Lassot, I I and 7 more authors.. (2001) ATF4 degradation relies on a phosphorylation-dependent interaction with the SCF(betaTrCP) ubiquitin ligase.. Molecular and cellular biology, [PMID:11238952] |
| 7. Siu, Fai F, Bain, Perry J PJ, LeBlanc-Chaffin, Rene R, Chen, Hong H and Kilberg, Michael S MS.. (2002) ATF4 is a mediator of the nutrient-sensing response pathway that activates the human asparagine synthetase gene.. The Journal of biological chemistry, (5): [PMID:11960987] |
| 8. Yang, Xiangli X and 11 more authors.. (2004) ATF4 is a substrate of RSK2 and an essential regulator of osteoblast biology; implication for Coffin-Lowry Syndrome.. Cell, (30): [PMID:15109498] |
| 9. Collins, John E JE and 12 more authors.. (2004) A genome annotation-driven approach to cloning the human ORFeome.. Genome biology, [PMID:15461802] |
| 10. Ohoka, Nobumichi N, Yoshii, Satoshi S, Hattori, Takayuki T, Onozaki, Kikuo K and Hayashi, Hidetoshi H.. (2005) TRB3, a novel ER stress-inducible gene, is induced via ATF4-CHOP pathway and is involved in cell death.. The EMBO journal, (23): [PMID:15775988] |
| 11. Yu, Vionnie W C VW and 7 more authors.. (2005) FIAT represses ATF4-mediated transcription to regulate bone mass in transgenic mice.. The Journal of cell biology, (23): [PMID:15911876] |
| 12. Lassot, Irina I and 5 more authors.. (2005) p300 modulates ATF4 stability and transcriptional activity independently of its acetyltransferase domain.. The Journal of biological chemistry, (16): [PMID:16219772] |
| 13. Sayer, John A JA and 41 more authors.. (2006) The centrosomal protein nephrocystin-6 is mutated in Joubert syndrome and activates transcription factor ATF4.. Nature genetics, [PMID:16682973] |
| 14. Köditz, Jens J and 8 more authors.. (2007) Oxygen-dependent ATF-4 stability is mediated by the PHD3 oxygen sensor.. Blood, (15): [PMID:17684156] |
| 15. Su, Nan N and Kilberg, Michael S MS.. (2008) C/EBP homology protein (CHOP) interacts with activating transcription factor 4 (ATF4) and negatively regulates the stress-dependent induction of the asparagine synthetase gene.. The Journal of biological chemistry, (12): [PMID:18940792] |
| 16. Vaz Meirelles, Gabriela G and 5 more authors.. (2010) Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome.. Journal of proteome research, (3): [PMID:20873783] |
| 17. Shoval, Yishay Y, Berissi, Hanna H, Kimchi, Adi A and Pietrokovski, Shmuel S.. (2011) New modularity of DAP-kinases: alternative splicing of the DRP-1 gene produces a ZIPk-like isoform.. PloS one, (8): [PMID:21408167] |
| 18. Fiebich, Bernd L BL and 6 more authors.. (2012) Pseudoephedrine inhibits T-cell activation by targeting NF-κB, NFAT and AP-1 signaling pathways.. Immunopharmacology and immunotoxicology, [PMID:21631396] |
| 19. D'Osualdo, Andrea and 6 more authors.. (2015) Transcription Factor ATF4 Induces NLRP1 Inflammasome Expression during Endoplasmic Reticulum Stress.. PloS one, [PMID:26086088] |
