| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab090020-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab090020-50μl | 50μl | In stock | $189.90 | |
| Ab090020-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab090020-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
pAb; Rabbit anti Human ATG7 Antibody; WB, IHC; Unconjugated
| Product Name | ATG7 Antibody - Primary antibody, specific to ATG7, Rabbit IgG |
|---|---|
| Synonyms | 1810013K23Rik antibody | Apg 7 antibody | APG7 autophagy 7 like antibody | APG7 autophagy 7-like (S. cerevisiae)antibody | APG7 like antibody | APG7, S. cerevisiae, homolog of antibody | APG7-like antibody | APG7L antibody | ATG 7 antibody | ATG12-activat |
| Specifications & Purity | ExactAb™, Validated, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | ATG7 |
| Immunogen | A synthetic peptide derived from human ATG7 (AA 691-703) |
| Positive Control | WB: Hep G2, THP-1, HeLa, Jurkat and HEK293 cell lysates. IHC: Human colon tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Rabbit anti Human ATG7 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
ATG7 Antibody (Ab090020) - Western Blot
All lanes: ATG7 Antibody (Ab090020) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 74 kDa
Exposure time: 36.9 s
ATG7 Antibody (Ab090020) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon using ATG7 Antibody (Ab090020). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202237 | Certificate of Analysis | Dec 26, 2023 | Ab090020 |
| ZJ23F1202238 | Certificate of Analysis | Dec 26, 2023 | Ab090020 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Buuh ZY, Lyu Z, Wang RE.. (2018) Interrogating the Roles of Post-Translational Modifications of Non-Histone Proteins.. J Med Chem, 61 (8): (3239-3252). [PMID:28505447] [10.1021/acs.jmedchem.6b01817] |
| 3. Yuan, W W, Stromhaug, P E PE and Dunn, W A WA.. (1999) Glucose-induced autophagy of peroxisomes in Pichia pastoris requires a unique E1-like protein.. Molecular biology of the cell, [PMID:10233149] |
| 4. Tanida, I I, Tanida-Miyake, E E, Ueno, T T and Kominami, E E.. (2001) The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3.. The Journal of biological chemistry, (19): [PMID:11096062] |
| 5. Tanida, Isei I, Tanida-Miyake, Emiko E, Komatsu, Masaaki M, Ueno, Takashi T and Kominami, Eiki E.. (2002) Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p.. The Journal of biological chemistry, (19): [PMID:11825910] |
| 6. Tanida, Isei I and 6 more authors.. (2002) Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs.. Biochemical and biophysical research communications, (22): [PMID:11890701] |
| 7. Sou, Yu-shin YS, Tanida, Isei I, Komatsu, Masaaki M, Ueno, Takashi T and Kominami, Eiki E.. (2006) Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16.. The Journal of biological chemistry, (10): [PMID:16303767] |
| 8. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997] |
| 9. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005] |
| 10. Lee, In Hye IH and Finkel, Toren T.. (2009) Regulation of autophagy by the p300 acetyltransferase.. The Journal of biological chemistry, (6): [PMID:19124466] |
| 11. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 12. Zhao, Ying Y and 9 more authors.. (2010) Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity.. Nature cell biology, [PMID:20543840] |
| 13. Tanida, Isei I, Yamasaki, Manabu M, Komatsu, Masaaki M and Ueno, Takashi T.. (2012) The FAP motif within human ATG7, an autophagy-related E1-like enzyme, is essential for the E2-substrate reaction of LC3 lipidation.. Autophagy, [PMID:22170151] |
| 14. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 15. Desai, Shruti S and 8 more authors.. (2013) Heat shock factor 1 (HSF1) controls chemoresistance and autophagy through transcriptional regulation of autophagy-related protein 7 (ATG7).. The Journal of biological chemistry, (29): [PMID:23386620] |
| 16. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 17. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
