Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG4; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab170495-100μg | 100μg | In stock | $49.90 | |
| Ab170495-1mg | 1mg | In stock | $240.90 | |
| Ab170495-5mg | 5mg | In stock | $606.90 | |
| Ab170495-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,005.90 |
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG4; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| Product Name | Atibuclimab (anti-CD14) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to CD14, >95%, high purity, Human IgG4 |
|---|---|
| Synonyms | CD 14 antibody; CD_antigen=CD14 antibody; CD14 antibody; CD14 antigen antibody; CD14 molecule antibody; CD14_HUMAN antibody; LPS-R antibody; Mo2 antibody; Monocyte differentiation antigen CD14 antibody; Monocyte differentiation antigen CD14 urinary form a |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot |
| Specificity | CD14 |
| Application | ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated |
| Action Type | ANTIBODY |
| Mechanism of action | Antibody of CD14 molecule |
| Product Description | Atibuclimab (anti-CD14) is a recombinant monoclonal against CD14. Atibuclimab can be used for the research of amyotrophic lateral sclerosis. Atibuclimab attenuates LPS-induced symptoms and strongly inhibits LPS-induced proinflammatory cytokine release, while only delaying the release of the anti-inflammatory cytokines soluble TNF receptor type I and IL-1 receptor antagonist. |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | Human IgG4 |
| Light Chain Type | kappa |
| SDS-PAGE | 28.0 kDa (Light Chain) & 51.0 kDa (Heavy Chain), under reducing conditions; 167.3 kDa, under non-reducing conditions. |
| Purification Method | Protein A purified |
| Purity | >95% |
| Source | CHO supernatant |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| CAS | 2417175-94-9 |
Atibuclimab (anti-CD14) (Ab170495) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD14 (red) with Atibuclimab (anti-CD14) (Ab170495). Goat Anti-Human IgG (FITC) (Ab175834) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
Atibuclimab (anti-CD14) (Ab170495) - SEC
The purity of Atibuclimab (anti-CD14) (Ab170495) is more than 95% verified by HPLC.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| IMGT/mAb-DB | 155 |
|---|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303669 | Certificate of Analysis | Mar 25, 2024 | Ab170495 |
| ZJ24F0303668 | Certificate of Analysis | Mar 25, 2024 | Ab170495 |
| ZJ24F0303667 | Certificate of Analysis | Mar 25, 2024 | Ab170495 |
| 1. Verbon A, Dekkers PE, ten Hove T, Hack CE, Pribble JP, Turner T, Souza S, Axtelle T, Hoek FJ, van Deventer SJ et al.. (2001) IC14, an anti-CD14 antibody, inhibits endotoxin-mediated symptoms and inflammatory responses in humans.. J Immunol, 166 (5): (3599-605). [PMID:11207321] |
| 2. Axtelle T, Pribble J. (2001) IC14, a CD14 specific monoclonal antibody, is a potential treatment for patients with severe sepsis.. J Endotoxin Res, 7 (4): (310-4). [PMID:11717588] |
| 3. Verbon A, Meijers JC, Spek CA, Hack CE, Pribble JP, Turner T, Dekkers PE, Axtelle T, Levi M, van Deventer SJ et al.. (2003) Effects of IC14, an anti-CD14 antibody, on coagulation and fibrinolysis during low-grade endotoxemia in humans.. J Infect Dis, 187 (1): (55-61). [PMID:12508146] |
| 4. Olszyna DP, Verbon A, Pribble JP, Turner T, Axtelle T, van Deventer SJ, van der Poll T. (2003) Effect of IC14, an anti-CD14 antibody, on plasma and cell-associated chemokines during human endotoxemia.. Eur Cytokine Netw, 14 (3): (158-62). [PMID:14656690] |
| 5. Axtelle T, Pribble J. (2003) An overview of clinical studies in healthy subjects and patients with severe sepsis with IC14, a CD14-specific chimeric monoclonal antibody.. J Endotoxin Res, 9 (6): (385-9). [PMID:14733726] |
| 6. Chan M, Hayashi T, Mathewson RD, Nour A, Hayashi Y, Yao S, Tawatao RI, Crain B, Tsigelny IF, Kouznetsova VL et al.. (2013) Identification of substituted pyrimido[5,4-b]indoles as selective Toll-like receptor 4 ligands.. J Med Chem, 56 (11): (4206-23). [PMID:23656327] |
| 7. Simmons DL, Tan S, Tenen DG, Nicholson-Weller A, Seed B. (1989) Monocyte antigen CD14 is a phospholipid anchored membrane protein.. Blood, 73 (1): (284-9). [PMID:2462937] |
| 8. Haziot A, Ferrero E, Köntgen F, Hijiya N, Yamamoto S, Silver J, Stewart CL, Goyert SM. (1996) Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice.. Immunity, 4 (4): (407-14). [PMID:8612135] |
| 9. Ferrero, E E and Goyert, S M SM.. (1988) Nucleotide sequence of the gene encoding the monocyte differentiation antigen, CD14.. Nucleic acids research, (11): [PMID:2453848] |
| 10. Setoguchi, M M and 5 more authors.. (1989) Mouse and human CD14 (myeloid cell-specific leucine-rich glycoprotein) primary structure deduced from cDNA clones.. Biochimica et biophysica acta, (7): [PMID:2472171] |
| 11. Bazil, V V and 6 more authors.. (1989) Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14.. Molecular immunology, [PMID:2779588] |
| 12. Haziot, A A and 5 more authors.. (1988) The monocyte differentiation antigen, CD14, is anchored to the cell membrane by a phosphatidylinositol linkage.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:3385210] |
| 13. da Silva Correia, J J, Soldau, K K, Christen, U U, Tobias, P S PS and Ulevitch, R J RJ.. (2001) Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2.. The Journal of biological chemistry, (15): [PMID:11274165] |
| 14. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952] |
| 15. Triantafilou, Martha M and 6 more authors.. (2006) Membrane sorting of toll-like receptor (TLR)-2/6 and TLR2/1 heterodimers at the cell surface determines heterotypic associations with CD36 and intracellular targeting.. The Journal of biological chemistry, (13): [PMID:16880211] |
| 16. Nakajima, Toshiaki T and 6 more authors.. (2008) Natural selection in the TLR-related genes in the course of primate evolution.. Immunogenetics, [PMID:18810425] |
| 17. Murakami, Kosaku K and 13 more authors.. (2012) Follistatin-related protein/follistatin-like 1 evokes an innate immune response via CD14 and toll-like receptor 4.. FEBS letters, (17): [PMID:22265692] |
| 18. Kelley, Stacy L SL, Lukk, Tiit T, Nair, Satish K SK and Tapping, Richard I RI.. (2013) The crystal structure of human soluble CD14 reveals a bent solenoid with a hydrophobic amino-terminal pocket.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:23264655] |
| 19. Estruch, Montserrat M and 5 more authors.. (2013) CD14 and TLR4 mediate cytokine release promoted by electronegative LDL in monocytes.. Atherosclerosis, [PMID:23880187] |
| 20. Kim, Sun-Mi SM, Kim, Bo-Young BY, Eo, Seong-Kug SK, Kim, Chi-Dae CD and Kim, Koanhoi K.. (2015) 27-Hydroxycholesterol up-regulates CD14 and predisposes monocytic cells to superproduction of CCL2 in response to lipopolysaccharide.. Biochimica et biophysica acta, [PMID:25497142] |
| 21. Yang, Linlin L and 12 more authors.. (2015) SP-R210 (Myo18A) Isoforms as Intrinsic Modulators of Macrophage Priming and Activation.. PloS one, [PMID:25965346] |
