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ATP5H Antibody - Primary antibody, specific to ATP5H, Rabbit IgG

Validated

ExactAb™

1.0 mg/mL

Application:

Features and benefits

Host species: Rabbit
Species reactivity(Reacts with): Human,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

Item Number

Ab090134

Grouped product items
SKU	Size	Availability	Price
Ab090134-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$79.90
Ab090134-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$229.90
Ab090134-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$359.90
Ab090134-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

pAb; Rabbit anti Human ATP5H Antibody; WB, IHC; Unconjugated

View related series

Click to view target information: ATP5PD

Accession#:O75947 ATP5H ATP5PD Gene ID:10476

Basic Description

Product Name	ATP5H Antibody - Primary antibody, specific to ATP5H, Rabbit IgG
Synonyms	ATP synthase D chain mitochondrial antibody \| ATP synthase H+ transporting mitochondrial F1F0 subunitantibody \| ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody \| ATP synthase subunit d antibody \| ATP synthase subunit d, mitochondrial an
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Host species	Rabbit
Specificity	ATP5H
Immunogen	A synthetic peptide derived from human ATP5H (AA 111-160).
Positive Control	WB: Hep G2, Jurkat and HeLa cell lysates. IHC: Human Squamous cell carcinoma of lung tissue.
Conjugation	Unconjugated
Grade	ExactAb™, Validated
Product Description	Rabbit anti Human ATP5H Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/2000 IHC: 1/100-1/300 Protein Function: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Immunogen affinity purified
Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

ATP5H Antibody (Ab090134) - Western Blot
All lanes: ATP5H Antibody (Ab090134) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 22 kDa
Exposure time: 14.0 s

ATP5H Antibody (Ab090134) - IHC
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung.
1. ATP5H Antibody (Ab090134) was diluted at 1/200 (4 ℃, overnight).
2. Tris-EDTA, pH 9.0 was used for antigen retrieval.
3. Secondary antibody was diluted at 1/200 (room temperature, 45 min).

Application

Dilution info

1/500-1/2000

IHC

1/100-1/300

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0910116	Certificate of Analysis	Sep 05, 2024	Ab090134
ZJ24F0910115	Certificate of Analysis	Sep 05, 2024	Ab090134

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e]
2. Zhang, Q H QH and 20 more authors.. (2000) Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.. Genome research, [PMID:11042152]
3. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569]
4. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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