A KLH conjugated synthetic peptide from the C-terminal region of human ATP6V1G1 (AA 90-118).
Positive Control
WB: MCF7, SK-N-SH, HeLa and C6 cell lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Product Description
Rabbit anti Human ATP6V1G1 Antibody, Polyclonal, could be used for WB and so on. Application: WB: 1/1000 Protein Function Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified + Peptide affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
ATP6J/ATP6V1G1 Antibody (Ab090147) - Western Blot All lanes: ATP6J/ATP6V1G1 Antibody (Ab090147) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 14 kDa Observed band size: 16 kDa Exposure time: 13.4 s
Associated Targets(Human)
ATP6V1G1TbioV-type proton ATPase subunit G 1 (0 Activities)
1.Qin A, Cheng TS, Pavlos NJ, Lin Z, Dai KR, Zheng MH. (2012) V-ATPases in osteoclasts: structure, function and potential inhibitors of bone resorption.. Int J Biochem Cell Biol, 44 (9):(1422-35). [PMID:22652318][10.1021/op500134e]
2.David, P P, Nguyen, H H, Barbier, A A and Baron, R R.. (1996) The bisphosphonate tiludronate is a potent inhibitor of the osteoclast vacuolar H(+)-ATPase.. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, [PMID:8889850]
3.Mao, M M and 16 more authors.. (1998) Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning.. Proceedings of the National Academy of Sciences of the United States of America, (7):[PMID:9653160]
4.Smith, Annabel N AN, Borthwick, Katherine J KJ and Karet, Fiona E FE.. (2002) Molecular cloning and characterization of novel tissue-specific isoforms of the human vacuolar H(+)-ATPase C, G and d subunits, and their evaluation in autosomal recessive distal renal tubular acidosis.. Gene, (4):[PMID:12384298]
5.Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27):[PMID:15164053]
6.Miles, Anna L AL, Burr, Stephen P SP, Grice, Guinevere L GL and Nathan, James A JA.. (2017) The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1α prolyl hydroxylation by regulating cellular iron levels.. eLife, (15):[PMID:28296633]
7.Duan, Xiaohong; Yang, Shaoqing; Zhang, Lei and Yang, Tielin.. (2018) V-ATPases and osteoclasts: ambiguous future of V-ATPases inhibitors in osteoporosis.. Theranostics, [PMID:30555553]
8.Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]
Solution Calculators
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.