pAb; Rabbit anti Human BDNF Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab090903-10μl | 10μl | In stock | $66.90 | |
| Ab090903-50μl | 50μl | In stock | $189.90 | |
| Ab090903-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab090903-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
pAb; Rabbit anti Human BDNF Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | BDNF Antibody - Primary antibody, specific to BDNF, Rabbit IgG |
|---|---|
| Synonyms | BDNF; ANON2; BULN2; Abrineurin antibody; ANON2 antibody; BDNF antibody; BDNF_HUMAN antibody; Brain Derived Neurotrophic Factor antibody; Brain-derived neurotrophic factor antibody; BULN2 antibody; MGC34632antibody; Neurotrophinantibody; BDNF Rabbit pAb |
| Specifications & Purity | ExactAb™, Validated, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | BDNF |
| Immunogen | A synthetic peptide derived from human BDNF (AA 150-200). |
| Positive Control | WB: Mouse heart, HeLa and THP-1 lysates. IHC: Human brain tissue. ICC/IF: HeLa cell |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Rabbit anti Human BDNF Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
BDNF Antibody (Ab090903) - Western Blot
All lanes: BDNF Antibody (Ab090903) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28, 30 kDa
Exposure time: 47.1 s
BDNF Antibody (Ab090903) - Western Blot
All lanes: BDNF Antibody (Ab090903) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa
Exposure time: 20.1 s
BDNF Antibody (Ab090903) - IF/ICC
Immunofluorescence analysis of BDNF in HeLa using BDNF Antibody (Ab090903), and cells were counterstained with DAPI (blue).
BDNF Antibody (Ab090903) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using BDNF Antibody (Ab090903). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303141 | Certificate of Analysis | Mar 13, 2024 | Ab090903 |
| ZJ24F0303140 | Certificate of Analysis | Mar 13, 2024 | Ab090903 |
| 1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 2. Shintani, A A, Ono, Y Y, Kaisho, Y Y and Igarashi, K K.. (1992) Characterization of the 5'-flanking region of the human brain-derived neurotrophic factor gene.. Biochemical and biophysical research communications, (15): [PMID:1339267] |
| 3. Maisonpierre, P C PC and 8 more authors.. (1991) Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations.. Genomics, [PMID:1889806] |
| 4. Hallböök, F F, Ibáñez, C F CF and Persson, H H.. (1991) Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary.. Neuron, [PMID:2025430] |
| 5. Jones, K R KR and Reichardt, L F LF.. (1990) Molecular cloning of a human gene that is a member of the nerve growth factor family.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2236018] |
| 6. Robinson, R C RC, Radziejewski, C C, Stuart, D I DI and Jones, E Y EY.. (1995) Structure of the brain-derived neurotrophic factor/neurotrophin 3 heterodimer.. Biochemistry, (4): [PMID:7703225] |
| 7. Rosenfeld, R D RD and 7 more authors.. (1995) Purification and identification of brain-derived neurotrophic factor from human serum.. Protein expression and purification, [PMID:8527932] |
| 8. Cargill, M M and 17 more authors.. (1999) Characterization of single-nucleotide polymorphisms in coding regions of human genes.. Nature genetics, [PMID:10391209] |
| 9. Robinson, R C RC and 8 more authors.. (1999) The structures of the neurotrophin 4 homodimer and the brain-derived neurotrophic factor/neurotrophin 4 heterodimer reveal a common Trk-binding site.. Protein science : a publication of the Protein Society, [PMID:10631974] |
| 10. Mowla, S J SJ and 6 more authors.. (2001) Biosynthesis and post-translational processing of the precursor to brain-derived neurotrophic factor.. The Journal of biological chemistry, (20): [PMID:11152678] |
| 11. Murphy, W J WJ and 5 more authors.. (2001) Molecular phylogenetics and the origins of placental mammals.. Nature, (1): [PMID:11214319] |
| 12. Weese-Mayer, Debra E DE, Bolk, Stacey S, Silvestri, Jean M JM and Chakravarti, Aravinda A.. (2002) Idiopathic congenital central hypoventilation syndrome: evaluation of brain-derived neurotrophic factor genomic DNA sequence variation.. American journal of medical genetics, (1): [PMID:11840487] |
| 13. Egan, Michael F MF and 11 more authors.. (2003) The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function.. Cell, (24): [PMID:12553913] |
| 14. Hall, Diana D, Dhilla, Alefiya A, Charalambous, Anna A, Gogos, Joseph A JA and Karayiorgou, Maria M.. (2003) Sequence variants of the brain-derived neurotrophic factor (BDNF) gene are strongly associated with obsessive-compulsive disorder.. American journal of human genetics, [PMID:12836135] |
| 15. Ribasés, M M and 9 more authors.. (2003) Met66 in the brain-derived neurotrophic factor (BDNF) precursor is associated with anorexia nervosa restrictive type.. Molecular psychiatry, [PMID:12888803] |
| 16. Sasaki, Ayako A and 13 more authors.. (2003) Molecular analysis of congenital central hypoventilation syndrome.. Human genetics, [PMID:14566559] |
| 17. Ribasés, Marta M and 27 more authors.. (2004) Association of BDNF with anorexia, bulimia and age of onset of weight loss in six European populations.. Human molecular genetics, (15): [PMID:15115760] |
| 18. Liu, Qing-Rong QR and 9 more authors.. (2005) Human brain derived neurotrophic factor (BDNF) genes, splicing patterns, and assessments of associations with substance abuse and Parkinson's Disease.. American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, (5): [PMID:15666411] |
| 19. Pruunsild, Priit P, Kazantseva, Anna A, Aid, Tamara T, Palm, Kaia K and Timmusk, Tõnis T.. (2007) Dissecting the human BDNF locus: bidirectional transcription, complex splicing, and multiple promoters.. Genomics, [PMID:17629449] |
| 20. Han, Joan C JC and 11 more authors.. (2008) Brain-derived neurotrophic factor and obesity in the WAGR syndrome.. The New England journal of medicine, (28): [PMID:18753648] |
| 21. Mandel, A L; Ozdener, H and Utermohlen, V.. (2009) Identification of pro- and mature brain-derived neurotrophic factor in human saliva.. Archives of oral biology, [PMID:19467646] |
| 22. Travaglia, Alessio A and 5 more authors.. (2012) Copper, BDNF and Its N-terminal domain: inorganic features and biological perspectives.. Chemistry (Weinheim an der Bergstrasse, Germany), (3): [PMID:23135810] |
| 23. Haile, C N CN and 11 more authors.. (2014) Plasma brain derived neurotrophic factor (BDNF) and response to ketamine in treatment-resistant depression.. The international journal of neuropsychopharmacology, [PMID:24103211] |
| 24. Glerup, Simon S and 22 more authors.. (2014) SorCS2 regulates dopaminergic wiring and is processed into an apoptotic two-chain receptor in peripheral glia.. Neuron, (4): [PMID:24908487] |
| 25. Harripaul, R R and 31 more authors.. (2018) Mapping autosomal recessive intellectual disability: combined microarray and exome sequencing identifies 26 novel candidate genes in 192 consanguineous families.. Molecular psychiatry, [PMID:28397838] |
| 26. Giza, Joanna I and 13 more authors.. (2018) The BDNF Val66Met Prodomain Disassembles Dendritic Spines Altering Fear Extinction Circuitry and Behavior.. Neuron, (11): [PMID:29909994] |
