mAb (2A4); Mouse anti Human BECN1 antibody; WB, IHC, Flow, ELISA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab090922-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $76.90 | |
| Ab090922-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $219.90 | |
| Ab090922-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $321.90 | |
| Ab090922-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,937.90 |
mAb (2A4); Mouse anti Human BECN1 antibody; WB, IHC, Flow, ELISA; Unconjugated
| Product Name | BECN1 Mouse mAb - Primary antibody, specific to BECN1, IgG1 |
|---|---|
| Synonyms | APG6 antibody; ATG 6 antibody; ATG6 antibody; ATG6 autophagy related 6 homolog antibody; Bcl-2-interacting protein beclin antibody; Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody; Beclin 1 autophagy related antibody; Beclin-1 antibod |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0mg/mL |
| Host species | Mouse |
| Specificity | BECN1 |
| Immunogen | Recombinant fragment corresponding to Human Beclin 1 aa 51-248. |
| Positive Control | WB: Hela, A431, MCF-7, Raji, Jurkat and SKBR-3 cell lysate; IHC: breast cancer tissues and liver cancer tissues; Flow: Raji cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Application |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
BECN1 Mouse mAb (Ab090922) - Western Blot
All lanes: BECN1 Mouse mAb (Ab090922) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
BECN1 Mouse mAb (Ab090922) - Flow Cytometry
Flow cytometric analysis of Raji cells using BECN1 Mouse mAb (Ab090922)
and negative control (purple).
BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.
BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| ELISA |
1/10000
|
| IHC |
1/200-1/1000
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700750 | Certificate of Analysis | Aug 01, 2023 | Ab090922 |
| ZJ23F0700751 | Certificate of Analysis | Aug 01, 2023 | Ab090922 |
| 1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] |
| 2. Ashkenazi A et al.. (2017) Polyglutamine tracts regulate beclin 1-dependent autophagy.. Nature, 545 (7652): (108-111). [PMID:28445460] |
| 3. Rommens, J M JM and 9 more authors.. (1995) Generation of a transcription map at the HSD17B locus centromeric to BRCA1 at 17q21.. Genomics, (10): [PMID:7490091] |
| 4. Liang, X H XH and 7 more authors.. (1998) Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein.. Journal of virology, [PMID:9765397] |
| 5. Aita, V M VM and 8 more authors.. (1999) Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21.. Genomics, (1): [PMID:10395800] |
| 6. Pattingre, Sophie S and 8 more authors.. (2005) Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy.. Cell, (23): [PMID:16179260] |
| 7. Oberstein, Adam A, Jeffrey, Philip D PD and Shi, Yigong Y.. (2007) Crystal structure of the Bcl-XL-Beclin 1 peptide complex: Beclin 1 is a novel BH3-only protein.. The Journal of biological chemistry, (27): [PMID:17337444] |
| 8. Maiuri, M Chiara MC and 12 more authors.. (2007) Functional and physical interaction between Bcl-X(L) and a BH3-like domain in Beclin-1.. The EMBO journal, (16): [PMID:17446862] |
| 9. Feng, Wei W, Huang, Siyi S, Wu, Hao H and Zhang, Mingjie M.. (2007) Molecular basis of Bcl-xL's target recognition versatility revealed by the structure of Bcl-xL in complex with the BH3 domain of Beclin-1.. Journal of molecular biology, (7): [PMID:17659302] |
| 10. Sauermann, M M and 10 more authors.. (2008) Reduced expression of vacuole membrane protein 1 affects the invasion capacity of tumor cells.. Oncogene, (21): [PMID:17724469] |
| 11. Orvedahl, Anthony A and 8 more authors.. (2007) HSV-1 ICP34.5 confers neurovirulence by targeting the Beclin 1 autophagy protein.. Cell host & microbe, (15): [PMID:18005679] |
| 12. Wei, Yongjie Y, Pattingre, Sophie S, Sinha, Sangita S, Bassik, Michael M and Levine, Beth B.. (2008) JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy.. Molecular cell, (20): [PMID:18570871] |
| 13. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 14. Sinha, Sangita S, Colbert, Christopher L CL, Becker, Nils N, Wei, Yongjie Y and Levine, Beth B.. (2008) Molecular basis of the regulation of Beclin 1-dependent autophagy by the gamma-herpesvirus 68 Bcl-2 homolog M11.. Autophagy, [PMID:18797192] |
| 15. Itakura, Eisuke E, Kishi, Chieko C, Inoue, Kinji K and Mizushima, Noboru N.. (2008) Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG.. Molecular biology of the cell, [PMID:18843052] |
| 16. Sun, Qiming Q and 5 more authors.. (2008) Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:19050071] |
| 17. Zalckvar, Einat E and 8 more authors.. (2009) DAP-kinase-mediated phosphorylation on the BH3 domain of beclin 1 promotes dissociation of beclin 1 from Bcl-XL and induction of autophagy.. EMBO reports, [PMID:19180116] |
| 18. Matsunaga, Kohichi K and 12 more authors.. (2009) Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages.. Nature cell biology, [PMID:19270696] |
| 19. Oppermann, Felix S FS and 7 more authors.. (2009) Large-scale proteomics analysis of the human kinome.. Molecular & cellular proteomics : MCP, [PMID:19369195] |
| 20. Luo, S S and Rubinsztein, D C DC.. (2010) Apoptosis blocks Beclin 1-dependent autophagosome synthesis: an effect rescued by Bcl-xL.. Cell death and differentiation, [PMID:19713971] |
| 21. Leib, David A DA, Alexander, Diane E DE, Cox, Douglas D, Yin, Jiyi J and Ferguson, Thomas A TA.. (2009) Interaction of ICP34.5 with Beclin 1 modulates herpes simplex virus type 1 pathogenesis through control of CD4+ T-cell responses.. Journal of virology, [PMID:19759141] |
| 22. Sagona, Antonia P AP and 8 more authors.. (2010) PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody.. Nature cell biology, [PMID:20208530] |
| 23. Thoresen, Sigrid B SB, Pedersen, Nina Marie NM, Liestøl, Knut K and Stenmark, Harald H.. (2010) A phosphatidylinositol 3-kinase class III sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates cytokinesis and degradative endocytic traffic.. Experimental cell research, (10): [PMID:20643123] |
| 24. Tang, Daolin D and 10 more authors.. (2010) Endogenous HMGB1 regulates autophagy.. The Journal of cell biology, (6): [PMID:20819940] |
| 25. Strappazzon, Flavie F and 8 more authors.. (2011) Mitochondrial BCL-2 inhibits AMBRA1-induced autophagy.. The EMBO journal, (6): [PMID:21358617] |
| 26. Wirawan, E and 13 more authors.. (2010) Caspase-mediated cleavage of Beclin-1 inactivates Beclin-1-induced autophagy and enhances apoptosis by promoting the release of proapoptotic factors from mitochondria.. Cell death & disease, [PMID:21364619] |
| 27. Li, Hua H and 8 more authors.. (2011) Following cytochrome c release, autophagy is inhibited during chemotherapy-induced apoptosis by caspase 8-mediated cleavage of Beclin 1.. Cancer research, (15): [PMID:21444671] |
| 28. Platta, Harald W HW, Abrahamsen, Hilde H, Thoresen, Sigrid B SB and Stenmark, Harald H.. (2012) Nedd4-dependent lysine-11-linked polyubiquitination of the tumour suppressor Beclin 1.. The Biochemical journal, (1): [PMID:21936852] |
| 29. Liu, Junli J and 19 more authors.. (2011) Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13.. Cell, (30): [PMID:21962518] |
| 30. Ma, Chunyan C and 5 more authors.. (2012) Receptor signaling lymphocyte-activation molecule family 1 (Slamf1) regulates membrane fusion and NADPH oxidase 2 (NOX2) activity by recruiting a Beclin-1/Vps34/ultraviolet radiation resistance-associated gene (UVRAG) complex.. The Journal of biological chemistry, (25): [PMID:22493499] |
| 31. Robert, Guillaume G and 10 more authors.. (2012) The anti-apoptotic Bcl-B protein inhibits BECN1-dependent autophagic cell death.. Autophagy, [PMID:22498477] |
| 32. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 33. Margariti, Andriana A and 18 more authors.. (2013) XBP1 mRNA splicing triggers an autophagic response in endothelial cells through BECLIN-1 transcriptional activation.. The Journal of biological chemistry, (11): [PMID:23184933] |
| 34. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 35. Fogel, Adam I AI and 8 more authors.. (2013) Role of membrane association and Atg14-dependent phosphorylation in beclin-1-mediated autophagy.. Molecular and cellular biology, [PMID:23878393] |
| 36. Xia, Pengyan P and 15 more authors.. (2013) WASH inhibits autophagy through suppression of Beclin 1 ubiquitination.. The EMBO journal, (16): [PMID:23974797] |
| 37. Seto, Shintaro S and 5 more authors.. (2013) Rab39a interacts with phosphatidylinositol 3-kinase and negatively regulates autophagy induced by lipopolysaccharide stimulation in macrophages.. PloS one, [PMID:24349490] |
| 38. Su, Minfei M and 5 more authors.. (2014) Targeting γ-herpesvirus 68 Bcl-2-mediated down-regulation of autophagy.. The Journal of biological chemistry, (21): [PMID:24443581] |
| 39. Mandell, Michael A MA and 13 more authors.. (2014) TRIM proteins regulate autophagy and can target autophagic substrates by direct recognition.. Developmental cell, (25): [PMID:25127057] |
| 40. McKnight, Nicole C NC and 9 more authors.. (2014) Beclin 1 is required for neuron viability and regulates endosome pathways via the UVRAG-VPS34 complex.. PLoS genetics, [PMID:25275521] |
| 41. Baskaran, Sulochanadevi S and 8 more authors.. (2014) Architecture and dynamics of the autophagic phosphatidylinositol 3-kinase complex.. eLife, (9): [PMID:25490155] |
| 42. Cianfanelli, Valentina V, D'Orazio, Melania M and Cecconi, Francesco F.. (2015) AMBRA1 and BECLIN 1 interplay in the crosstalk between autophagy and cell proliferation.. Cell cycle (Georgetown, Tex.), [PMID:25803737] |
| 43. Chauhan, Santosh S, Mandell, Michael A MA and Deretic, Vojo V.. (2015) IRGM governs the core autophagy machinery to conduct antimicrobial defense.. Molecular cell, (7): [PMID:25891078] |
| 44. Siddiqui, Mohammad Adnan MA, Mukherjee, Sushovita S, Manivannan, Praveen P and Malathi, Krishnamurthy K.. (2015) RNase L Cleavage Products Promote Switch from Autophagy to Apoptosis by Caspase-Mediated Cleavage of Beclin-1.. International journal of molecular sciences, (31): [PMID:26263979] |
| 45. Kimura, Tomonori T and 6 more authors.. (2015) TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity.. The Journal of cell biology, (14): [PMID:26347139] |
| 46. Liu, Kai K and 12 more authors.. (2016) Negative regulation of phosphatidylinositol 3-phosphate levels in early-to-late endosome conversion.. The Journal of cell biology, (18): [PMID:26783301] |
| 47. Mandell, Michael A MA and 8 more authors.. (2016) TRIM17 contributes to autophagy of midbodies while actively sparing other targets from degradation.. Journal of cell science, (1): [PMID:27562068] |
| 48. Chauhan, Santosh S and 11 more authors.. (2016) TRIMs and Galectins Globally Cooperate and TRIM16 and Galectin-3 Co-direct Autophagy in Endomembrane Damage Homeostasis.. Developmental cell, (10): [PMID:27693506] |
| 49. Tian, Miao M and 5 more authors.. (2017) Beclin1 antagonizes LAPTM4B-mediated EGFR overactivation in gastric cancer cells.. Gene, (30): [PMID:28479384] |
| 50. Fusco, Carmela C and 13 more authors.. (2018) TRIM50 regulates Beclin 1 proautophagic activity.. Biochimica et biophysica acta. Molecular cell research, [PMID:29604308] |
| 51. Di Rienzo, M M and 22 more authors.. (2019) Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains.. Science advances, [PMID:31123703] |
| 52. Miao, Guangyan; Zhang, Yujie; Chen, Di and Zhang, Hong.. (2020) The ER-Localized Transmembrane Protein TMEM39A/SUSR2 Regulates Autophagy by Controlling the Trafficking of the PtdIns(4)P Phosphatase SAC1.. Molecular cell, (6): [PMID:31806350] |
