| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab090922-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $76.90 | |
| Ab090922-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $219.90 | |
| Ab090922-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $321.90 | |
| Ab090922-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,937.90 |
mAb (2A4); Mouse anti Human BECN1 Antibody; WB, IHC, Flow Cytometry, ELISA; Unconjugated
| Product Name | BECN1 Mouse mAb - Primary antibody, specific to BECN1, IgG1 |
|---|---|
| Synonyms | Coiled-coil myosin-like BCL2-interacting protein | Protein GT197 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0mg/mL |
| Biochemical and Physiological Mechanisms | Plays a central role in autophagy. Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved |
| Host species | Mouse |
| Specificity | BECN1 |
| Immunogen | Recombinant protein of human BECN1 (AA 51-248). |
| Positive Control | WB: Hela, A431, MCF-7, Raji, Jurkat and SKBR-3 cell lysate; IHC: breast cancer tissues and liver cancer tissues; Flow: Raji cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human BECN1 Antibody, Monoclonal (2A4), could be used for WB, IHC, Flow Cytometry, ELISA and so on.
|
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
BECN1 Mouse mAb (Ab090922) - Western Blot
All lanes: BECN1 Mouse mAb (Ab090922) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
BECN1 Mouse mAb (Ab090922) - Flow Cytometry
Flow cytometric analysis of Raji cells using BECN1 Mouse mAb (Ab090922)
and negative control (purple).
BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.
BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| ELISA |
1/10000
">
1/10000
|
| IHC |
1/200-1/1000
|
| Flow Cytometry |
1/200-1/400
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700750 | Certificate of Analysis | Aug 01, 2023 | Ab090922 |
| ZJ23F0700751 | Certificate of Analysis | Aug 01, 2023 | Ab090922 |
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| 29. Liu, Junli J and 19 more authors.. (2011) Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13.. Cell, (30): [PMID:21962518] |
| 30. Ma, Chunyan C and 5 more authors.. (2012) Receptor signaling lymphocyte-activation molecule family 1 (Slamf1) regulates membrane fusion and NADPH oxidase 2 (NOX2) activity by recruiting a Beclin-1/Vps34/ultraviolet radiation resistance-associated gene (UVRAG) complex.. The Journal of biological chemistry, (25): [PMID:22493499] |
| 31. Robert, Guillaume G and 10 more authors.. (2012) The anti-apoptotic Bcl-B protein inhibits BECN1-dependent autophagic cell death.. Autophagy, [PMID:22498477] |
| 32. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 33. Margariti, Andriana A and 18 more authors.. (2013) XBP1 mRNA splicing triggers an autophagic response in endothelial cells through BECLIN-1 transcriptional activation.. The Journal of biological chemistry, (11): [PMID:23184933] |
| 34. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
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| 36. Xia, Pengyan P and 15 more authors.. (2013) WASH inhibits autophagy through suppression of Beclin 1 ubiquitination.. The EMBO journal, (16): [PMID:23974797] |
| 37. Seto, Shintaro S and 5 more authors.. (2013) Rab39a interacts with phosphatidylinositol 3-kinase and negatively regulates autophagy induced by lipopolysaccharide stimulation in macrophages.. PloS one, [PMID:24349490] |
| 38. Su, Minfei M and 5 more authors.. (2014) Targeting γ-herpesvirus 68 Bcl-2-mediated down-regulation of autophagy.. The Journal of biological chemistry, (21): [PMID:24443581] |
| 39. Mandell, Michael A MA and 13 more authors.. (2014) TRIM proteins regulate autophagy and can target autophagic substrates by direct recognition.. Developmental cell, (25): [PMID:25127057] |
| 40. McKnight, Nicole C NC and 9 more authors.. (2014) Beclin 1 is required for neuron viability and regulates endosome pathways via the UVRAG-VPS34 complex.. PLoS genetics, [PMID:25275521] |
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