mAb (2A4); Mouse anti Human BECN1 antibody; WB, IHC, Flow, ELISA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab090922-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $76.90 | |
| Ab090922-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $219.90 | |
| Ab090922-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $321.90 | |
| Ab090922-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,937.90 |
mAb (2A4); Mouse anti Human BECN1 antibody; WB, IHC, Flow, ELISA; Unconjugated
| Product Name | BECN1 Mouse mAb - Primary antibody, specific to BECN1, IgG1 |
|---|---|
| Synonyms | APG6 antibody; ATG 6 antibody; ATG6 antibody; ATG6 autophagy related 6 homolog antibody; Bcl-2-interacting protein beclin antibody; Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody; Beclin 1 autophagy related antibody; Beclin-1 antibod |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0mg/mL |
| Host species | Mouse |
| Specificity | BECN1 |
| Immunogen | Recombinant fragment corresponding to Human Beclin 1 aa 51-248. |
| Positive Control | WB: Hela, A431, MCF-7, Raji, Jurkat and SKBR-3 cell lysate; IHC: breast cancer tissues and liver cancer tissues; Flow: Raji cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Application |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
BECN1 Mouse mAb (Ab090922) - Western Blot
All lanes: BECN1 Mouse mAb (Ab090922) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
BECN1 Mouse mAb (Ab090922) - Flow Cytometry
Flow cytometric analysis of Raji cells using BECN1 Mouse mAb (Ab090922)
and negative control (purple).
BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.
BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| ELISA |
1/10000
|
| IHC |
1/200-1/1000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700750 | Certificate of Analysis | Aug 01, 2023 | Ab090922 |
| ZJ23F0700751 | Certificate of Analysis | Aug 01, 2023 | Ab090922 |
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| 24. Tang, Daolin D and 10 more authors.. (2010) Endogenous HMGB1 regulates autophagy.. The Journal of cell biology, (6): [PMID:20819940] |
| 25. Strappazzon, Flavie F and 8 more authors.. (2011) Mitochondrial BCL-2 inhibits AMBRA1-induced autophagy.. The EMBO journal, (6): [PMID:21358617] |
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