mAb (C1); Mouse anti Human Beta 2 Microglobulin Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab090968-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab090968-20μl | 20μl | In stock | $99.90 | |
| Ab090968-50μl | 50μl | In stock | $189.90 | |
| Ab090968-100μl | 100μl | In stock | $299.90 | |
| Ab090968-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
mAb (C1); Mouse anti Human Beta 2 Microglobulin Antibody; WB, IHC; Unconjugated
| Product Name | Beta 2 Microglobulin Mouse mAb - Primary antibody, specific to B2M, Mouse IgG2a |
|---|---|
| Synonyms | B2M antibody; B2MG_HUMAN antibody; Beta 2 microglobin antibody; Beta 2 microglobulin antibody; Beta 2 microglobulin precursor antibody; Beta chain of mhc class 1 proteins antibody; Beta chain of MHC class I molecules antibody; Beta-2-microglobulin form pI |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | B2M |
| Immunogen | Recombinant B2m expressed in E.coli (AA 22-119) |
| Positive Control | WB: A431, THP-1, HeLa and Jurkat cell lysates. IHC: Human cardiac muscle, liver, kidney tissues. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Mouse anti Human Beta 2 Microglobulin Antibody, Monoclonal (C1), could be used for WB, IHC and so on. Application: WB: 0.01-2µg/mL IHC: 5-40µg/mL Protein Function Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Beta 2 Microglobulin Mouse mAb (Ab090968) - Western Blot
All lanes: Beta 2 Microglobulin Mouse mAb (Ab090968) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 12 kDa
Exposure time: 3.4 s
Beta 2 Microglobulin Mouse mAb (Ab090968) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 40 µg/mL Beta 2 Microglobulin Mouse mAb (Ab090968)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Beta 2 Microglobulin Mouse mAb (Ab090968) - IHC
DAB staining on IHC
Sample: Human Liver Tissue
Primary Ab: 40 µg/mL Beta 2 Microglobulin Mouse mAb (Ab090968)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Beta 2 Microglobulin Mouse mAb (Ab090968) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 40 µg/mL Beta 2 Microglobulin Mouse mAb (Ab090968)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
| IHC | 5-40µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202051 | Certificate of Analysis | Dec 14, 2023 | Ab090968 |
| ZJ23F1202052 | Certificate of Analysis | Dec 14, 2023 | Ab090968 |
| ZJ23F1202053 | Certificate of Analysis | Dec 14, 2023 | Ab090968 |
| 1. Argilés, A A, Derancourt, J J, Jauregui-Adell, J J, Mion, C C and Demaille, J G JG.. (1992) Biochemical characterization of serum and urinary beta 2 microglobulin in end-stage renal disease patients.. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, [PMID:1336137] |
| 2. Okon, M M, Bray, P P and Vucelić, D D.. (1992) 1H NMR assignments and secondary structure of human beta 2-microglobulin in solution.. Biochemistry, (22): [PMID:1390678] |
| 3. Saper, M A MA, Bjorkman, P J PJ and Wiley, D C DC.. (1991) Refined structure of the human histocompatibility antigen HLA-A2 at 2.6 A resolution.. Journal of molecular biology, (20): [PMID:2038058] |
| 4. Bjorkman, P J PJ and 5 more authors.. (1987) Structure of the human class I histocompatibility antigen, HLA-A2.. Nature, [PMID:3309677] |
| 5. Güssow, D D and 6 more authors.. (1987) The human beta 2-microglobulin gene. Primary structure and definition of the transcriptional unit.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:3312414] |
| 6. Gorevic, P D PD and 8 more authors.. (1986) Polymerization of intact beta 2-microglobulin in tissue causes amyloidosis in patients on chronic hemodialysis.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3532124] |
| 7. Cunningham, B A BA, Wang, J L JL, Berggård, I I and Peterson, P A PA.. (1973) The complete amino acid sequence of beta 2-microglobulin.. Biochemistry, (20): [PMID:4586824] |
| 8. Suggs, S V SV, Wallace, R B RB, Hirose, T T, Kawashima, E H EH and Itakura, K K.. (1981) Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6171820] |
| 9. Momoi, T T and 5 more authors.. (1995) Amino acid sequence of a modified beta 2-microglobulin in renal failure patient urine and long-term dialysis patient blood.. Clinica chimica acta; international journal of clinical chemistry, (15): [PMID:7554280] |
| 10. Collins, E J EJ, Garboczi, D N DN, Karpusas, M N MN and Wiley, D C DC.. (1995) The three-dimensional structure of a class I major histocompatibility complex molecule missing the alpha 3 domain of the heavy chain.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:7862664] |
| 11. Miyata, T T and 7 more authors.. (1994) Glycation of human beta 2-microglobulin in patients with hemodialysis-associated amyloidosis: identification of the glycated sites.. Biochemistry, (11): [PMID:7918443] |
| 12. Haag-Weber, M M, Mai, B B and Hörl, W H WH.. (1994) Isolation of a granulocyte inhibitory protein from uraemic patients with homology of beta 2-microglobulin.. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, [PMID:8084451] |
| 13. Smith, K J KJ and 6 more authors.. (1996) Bound water structure and polymorphic amino acids act together to allow the binding of different peptides to MHC class I HLA-B53.. Immunity, [PMID:8624812] |
| 14. Braud, V M VM, Allan, D S DS, Wilson, D D and McMichael, A J AJ.. (1998) TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide.. Current biology : CB, (1): [PMID:9427624] |
| 15. Wainwright, S D SD, Biro, P A PA and Holmes, C H CH.. (2000) HLA-F is a predominantly empty, intracellular, TAP-associated MHC class Ib protein with a restricted expression pattern.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:10605026] |
| 16. Rosa, F F and 5 more authors.. (1983) The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.. The EMBO journal, [PMID:11894933] |
| 17. Trinh, Chi H CH, Smith, David P DP, Kalverda, Arnout P AP, Phillips, Simon E V SE and Radford, Sheena E SE.. (2002) Crystal structure of monomeric human beta-2-microglobulin reveals clues to its amyloidogenic properties.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:12119416] |
| 18. Stewart-Jones, Guillaume B E GB, McMichael, Andrew J AJ, Bell, John I JI, Stuart, David I DI and Jones, E Yvonne EY.. (2003) A structural basis for immunodominant human T cell receptor recognition.. Nature immunology, [PMID:12796775] |
| 19. He, Xian-Hui XH, Xu, Li-Hui LH, Liu, Yi Y and Zeng, Yao-Ying YY.. (2004) [Cloning of human beta-microglobulin gene and its high expression in Escherichia coli].. Sheng wu gong cheng xue bao = Chinese journal of biotechnology, [PMID:16108498] |
| 20. Eakin, Catherine M CM, Berman, Andrea J AJ and Miranker, Andrew D AD.. (2006) A native to amyloidogenic transition regulated by a backbone trigger.. Nature structural & molecular biology, [PMID:16491088] |
| 21. Deng, Nan-Jie NJ, Yan, Lisa L, Singh, Deepak D and Cieplak, Piotr P.. (2006) Molecular basis for the Cu2+ binding-induced destabilization of beta2-microglobulin revealed by molecular dynamics simulation.. Biophysical journal, (1): [PMID:16513784] |
| 22. Wani, Manzoor A MA and 8 more authors.. (2006) Familial hypercatabolic hypoproteinemia caused by deficiency of the neonatal Fc receptor, FcRn, due to a mutant beta2-microglobulin gene.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:16549777] |
| 23. Kihara, Miho M and 7 more authors.. (2006) Conformation of amyloid fibrils of beta2-microglobulin probed by tryptophan mutagenesis.. The Journal of biological chemistry, (13): [PMID:16901902] |
| 24. Shiroishi, Mitsunori M and 8 more authors.. (2006) Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d).. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:17056715] |
| 25. Iwata, Kentaro K, Matsuura, Takanori T, Sakurai, Kazumasa K, Nakagawa, Atsushi A and Goto, Yuji Y.. (2007) High-resolution crystal structure of beta2-microglobulin formed at pH 7.0.. Journal of biochemistry, [PMID:17646174] |
| 26. Esposito, Gennaro G and 16 more authors.. (2008) The controlling roles of Trp60 and Trp95 in beta2-microglobulin function, folding and amyloid aggregation properties.. Journal of molecular biology, (9): [PMID:18395224] |
| 27. Ricagno, Stefano S and 7 more authors.. (2008) DE loop mutations affect beta2-microglobulin stability and amyloid aggregation.. Biochemical and biophysical research communications, (5): [PMID:18835253] |
| 28. Ricagno, Stefano S, Raimondi, Sara S, Giorgetti, Sofia S, Bellotti, Vittorio V and Bolognesi, Martino M.. (2009) Human beta-2 microglobulin W60V mutant structure: Implications for stability and amyloid aggregation.. Biochemical and biophysical research communications, (13): [PMID:19284997] |
| 29. Giorgetti, Sofia S and 17 more authors.. (2011) Effect of tetracyclines on the dynamics of formation and destructuration of beta2-microglobulin amyloid fibrils.. The Journal of biological chemistry, (21): [PMID:21068391] |
| 30. Valleix, Sophie S and 25 more authors.. (2012) Hereditary systemic amyloidosis due to Asp76Asn variant β2-microglobulin.. The New England journal of medicine, (14): [PMID:22693999] |
| 31. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 32. Sreejit, Gopalkrishna G and 6 more authors.. (2014) The ESAT-6 protein of Mycobacterium tuberculosis interacts with beta-2-microglobulin (β2M) affecting antigen presentation function of macrophage.. PLoS pathogens, [PMID:25356553] |
| 33. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
