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Beta 2 Microglobulin Mouse mAb - Primary antibody, specific to B2M, Mouse IgG2a

  • Carrier Free
  • ExactAb™
  • High Performance
  • Validated
  • Lot by Lot
Features and benefits
  • Short Overview:

    mAb (C1); Mouse anti Human Beta 2 Microglobulin Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human,Pig
  • Isotype: Mouse IgG2a
    Application:
  • IHC
  • WB
Item Number
Ab090968
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab090968-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab090968-20μl
20μl
In stock
$99.90
Ab090968-50μl
50μl
In stock
$189.90
Ab090968-100μl
100μl
In stock
$299.90
Ab090968-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,199.90

mAb (C1); Mouse anti Human Beta 2 Microglobulin Antibody; WB, IHC; Unconjugated

Basic Description

Product NameBeta 2 Microglobulin Mouse mAb - Primary antibody, specific to B2M, Mouse IgG2a
SynonymsB2M antibody; B2MG_HUMAN antibody; Beta 2 microglobin antibody; Beta 2 microglobulin antibody; Beta 2 microglobulin precursor antibody; Beta chain of mhc class 1 proteins antibody; Beta chain of MHC class I molecules antibody; Beta-2-microglobulin form pI
Specifications & PurityExactAb™, Validated, Carrier Free, High performance, Lot by Lot
Host speciesMouse
SpecificityB2M
ImmunogenRecombinant B2m expressed in E.coli (AA 22-119)
Positive ControlWB: A431, THP-1, HeLa and Jurkat cell lysates. IHC: Human cardiac muscle, liver, kidney tissues.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human Beta 2 Microglobulin Antibody, Monoclonal (C1), could be used for WB, IHC and so on.

Application:

WB: 0.01-2µg/mL IHC: 5-40µg/mL

Protein Function

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

Product Properties

IsotypeMouse IgG2a
Light Chain Typekappa
Purification MethodProtein A + Protein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Beta 2 Microglobulin Mouse mAb (Ab090968) - Western Blot
All lanes: Beta 2 Microglobulin Mouse mAb (Ab090968) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 14 kDa
Observed band size: 12 kDa
Exposure time: 3.4 s

Beta 2 Microglobulin Mouse mAb (Ab090968) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 40 µg/mL Beta 2 Microglobulin Mouse mAb (Ab090968)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Beta 2 Microglobulin Mouse mAb (Ab090968) - IHC
DAB staining on IHC
Sample: Human Liver Tissue
Primary Ab: 40 µg/mL Beta 2 Microglobulin Mouse mAb (Ab090968)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Beta 2 Microglobulin Mouse mAb (Ab090968) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 40 µg/mL Beta 2 Microglobulin Mouse mAb (Ab090968)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Associated Targets

B2M Tbio Beta-2-microglobulin 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

0.01-2 µg/mL

IHC

5-40µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

3 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202051Certificate of AnalysisDec 14, 2023 Ab090968
ZJ23F1202052Certificate of AnalysisDec 14, 2023 Ab090968
ZJ23F1202053Certificate of AnalysisDec 14, 2023 Ab090968

Related Documents

References

1. Argilés, A A, Derancourt, J J, Jauregui-Adell, J J, Mion, C C and Demaille, J G JG..  (1992)  Biochemical characterization of serum and urinary beta 2 microglobulin in end-stage renal disease patients..  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,      [PMID:1336137]
2. Okon, M M, Bray, P P and Vucelić, D D..  (1992)  1H NMR assignments and secondary structure of human beta 2-microglobulin in solution..  Biochemistry,    (22):   [PMID:1390678]
3. Saper, M A MA, Bjorkman, P J PJ and Wiley, D C DC..  (1991)  Refined structure of the human histocompatibility antigen HLA-A2 at 2.6 A resolution..  Journal of molecular biology,    (20):   [PMID:2038058]
4. Bjorkman, P J PJ and 5 more authors..  (1987)  Structure of the human class I histocompatibility antigen, HLA-A2..  Nature,      [PMID:3309677]
5. Güssow, D D and 6 more authors..  (1987)  The human beta 2-microglobulin gene. Primary structure and definition of the transcriptional unit..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:3312414]
6. Gorevic, P D PD and 8 more authors..  (1986)  Polymerization of intact beta 2-microglobulin in tissue causes amyloidosis in patients on chronic hemodialysis..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3532124]
7. Cunningham, B A BA, Wang, J L JL, Berggård, I I and Peterson, P A PA..  (1973)  The complete amino acid sequence of beta 2-microglobulin..  Biochemistry,    (20):   [PMID:4586824]
8. Suggs, S V SV, Wallace, R B RB, Hirose, T T, Kawashima, E H EH and Itakura, K K..  (1981)  Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:6171820]
9. Momoi, T T and 5 more authors..  (1995)  Amino acid sequence of a modified beta 2-microglobulin in renal failure patient urine and long-term dialysis patient blood..  Clinica chimica acta; international journal of clinical chemistry,    (15):   [PMID:7554280]
10. Collins, E J EJ, Garboczi, D N DN, Karpusas, M N MN and Wiley, D C DC..  (1995)  The three-dimensional structure of a class I major histocompatibility complex molecule missing the alpha 3 domain of the heavy chain..  Proceedings of the National Academy of Sciences of the United States of America,    (14):   [PMID:7862664]
11. Miyata, T T and 7 more authors..  (1994)  Glycation of human beta 2-microglobulin in patients with hemodialysis-associated amyloidosis: identification of the glycated sites..  Biochemistry,    (11):   [PMID:7918443]
12. Haag-Weber, M M, Mai, B B and Hörl, W H WH..  (1994)  Isolation of a granulocyte inhibitory protein from uraemic patients with homology of beta 2-microglobulin..  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,      [PMID:8084451]
13. Smith, K J KJ and 6 more authors..  (1996)  Bound water structure and polymorphic amino acids act together to allow the binding of different peptides to MHC class I HLA-B53..  Immunity,      [PMID:8624812]
14. Braud, V M VM, Allan, D S DS, Wilson, D D and McMichael, A J AJ..  (1998)  TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide..  Current biology : CB,    (1):   [PMID:9427624]
15. Wainwright, S D SD, Biro, P A PA and Holmes, C H CH..  (2000)  HLA-F is a predominantly empty, intracellular, TAP-associated MHC class Ib protein with a restricted expression pattern..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:10605026]
16. Rosa, F F and 5 more authors..  (1983)  The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon..  The EMBO journal,      [PMID:11894933]
17. Trinh, Chi H CH, Smith, David P DP, Kalverda, Arnout P AP, Phillips, Simon E V SE and Radford, Sheena E SE..  (2002)  Crystal structure of monomeric human beta-2-microglobulin reveals clues to its amyloidogenic properties..  Proceedings of the National Academy of Sciences of the United States of America,    (23):   [PMID:12119416]
18. Stewart-Jones, Guillaume B E GB, McMichael, Andrew J AJ, Bell, John I JI, Stuart, David I DI and Jones, E Yvonne EY..  (2003)  A structural basis for immunodominant human T cell receptor recognition..  Nature immunology,      [PMID:12796775]
19. He, Xian-Hui XH, Xu, Li-Hui LH, Liu, Yi Y and Zeng, Yao-Ying YY..  (2004)  [Cloning of human beta-microglobulin gene and its high expression in Escherichia coli]..  Sheng wu gong cheng xue bao = Chinese journal of biotechnology,      [PMID:16108498]
20. Eakin, Catherine M CM, Berman, Andrea J AJ and Miranker, Andrew D AD..  (2006)  A native to amyloidogenic transition regulated by a backbone trigger..  Nature structural & molecular biology,      [PMID:16491088]
21. Deng, Nan-Jie NJ, Yan, Lisa L, Singh, Deepak D and Cieplak, Piotr P..  (2006)  Molecular basis for the Cu2+ binding-induced destabilization of beta2-microglobulin revealed by molecular dynamics simulation..  Biophysical journal,    (1):   [PMID:16513784]
22. Wani, Manzoor A MA and 8 more authors..  (2006)  Familial hypercatabolic hypoproteinemia caused by deficiency of the neonatal Fc receptor, FcRn, due to a mutant beta2-microglobulin gene..  Proceedings of the National Academy of Sciences of the United States of America,    (28):   [PMID:16549777]
23. Kihara, Miho M and 7 more authors..  (2006)  Conformation of amyloid fibrils of beta2-microglobulin probed by tryptophan mutagenesis..  The Journal of biological chemistry,    (13):   [PMID:16901902]
24. Shiroishi, Mitsunori M and 8 more authors..  (2006)  Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d)..  Proceedings of the National Academy of Sciences of the United States of America,    (31):   [PMID:17056715]
25. Iwata, Kentaro K, Matsuura, Takanori T, Sakurai, Kazumasa K, Nakagawa, Atsushi A and Goto, Yuji Y..  (2007)  High-resolution crystal structure of beta2-microglobulin formed at pH 7.0..  Journal of biochemistry,      [PMID:17646174]
26. Esposito, Gennaro G and 16 more authors..  (2008)  The controlling roles of Trp60 and Trp95 in beta2-microglobulin function, folding and amyloid aggregation properties..  Journal of molecular biology,    (9):   [PMID:18395224]
27. Ricagno, Stefano S and 7 more authors..  (2008)  DE loop mutations affect beta2-microglobulin stability and amyloid aggregation..  Biochemical and biophysical research communications,    (5):   [PMID:18835253]
28. Ricagno, Stefano S, Raimondi, Sara S, Giorgetti, Sofia S, Bellotti, Vittorio V and Bolognesi, Martino M..  (2009)  Human beta-2 microglobulin W60V mutant structure: Implications for stability and amyloid aggregation..  Biochemical and biophysical research communications,    (13):   [PMID:19284997]
29. Giorgetti, Sofia S and 17 more authors..  (2011)  Effect of tetracyclines on the dynamics of formation and destructuration of beta2-microglobulin amyloid fibrils..  The Journal of biological chemistry,    (21):   [PMID:21068391]
30. Valleix, Sophie S and 25 more authors..  (2012)  Hereditary systemic amyloidosis due to Asp76Asn variant β2-microglobulin..  The New England journal of medicine,    (14):   [PMID:22693999]
31. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,    (16):   [PMID:24275569]
32. Sreejit, Gopalkrishna G and 6 more authors..  (2014)  The ESAT-6 protein of Mycobacterium tuberculosis interacts with beta-2-microglobulin (β2M) affecting antigen presentation function of macrophage..  PLoS pathogens,      [PMID:25356553]

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