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beta Actin Mouse mAb (Biotin) - Primary antibody, specific to beta actin, IgG1

  • Azide Free
  • ExactAb™
  • High performance
  • Validated
  • 1.0 mg/mL
Features and benefits
  • Short Overview:

    mAb ( FG01/2D10); Mouse anti Human beta actin Antibody; WB, IF, Flow; Biotin

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: IgG1
    Application:
  • Flow Cyt
  • ICC/IF
  • WB
Item Number
Ab175850
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab175850-10μl (Trial Size)
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10μl
In stock
$66.90
Ab175850-20μl
20μl
In stock
$99.90
Ab175850-50μl
50μl
In stock
$199.90
Ab175850-100μl
100μl
In stock
$299.90
Ab175850-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,499.90

mAb ( FG01/2D10); Mouse anti Human beta actin Antibody; WB, IF, Flow; Biotin

Basic Description

Product Namebeta Actin Mouse mAb (Biotin) - Primary antibody, specific to beta actin, IgG1
SynonymsA26C1A antibody|A26C1B antibody|ACTB antibody|ACTB_HUMAN antibody|Actin beta antibody|Actin cytoplasmic 1 antibody|Actin, cytoplasmic 1, N-terminally processed antibody|Actx antibody|b actin antibody|b-actin antibody|Beta cytoskeletal actin antibody|Beta-
Specifications & PurityExactAb™, Validated, Azide Free, High performance, 1.0 mg/mL
Host speciesMouse
Specificitybeta actin
ImmunogenSynthetic peptide in beta Actin
Positive ControlWB: K562, A549, HeLa, Jurkat, NIH3T3 and C6 whole cell lysates Flow: Hela IF: Hela
ConjugationBiotin
GradeAzide Free, ExactAb™, High performance, Validated
Product Description

Application:
WB :1:5000-1:40000
IF: 1:2000
Flow: 1:500-1:2500

Product Properties

IsotypeIgG1
Purification MethodProtein A purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageStore at 4℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

beta Actin Mouse mAb (Biotin) (Ab175850) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/2000 dilution in blocking buffer overnight at 4°C. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

beta Actin Mouse mAb (Biotin) (Ab175850) - Western Blot
All lanes: beta Actin Mouse mAb (Biotin) (Ab175850) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
SA-HRP: 1:20000

Predicted band size: 42.0 kDa
Observed band size: 42.0 kDa
Exposure time: 12.1s

beta Actin Mouse mAb (Biotin) (Ab175850) - Flow Cytometry
Flow cytometry analysis of Beta Actin in Hela cells (red) compared to an isotype control (blue). The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/100 dilution for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/2000 for 30 minutes at 4°C in the dark.

Associated Targets

TUBB Tclin Tubulin beta chain 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

ACTB Tbio Actin, cytoplasmic 1 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
Flow

1:500-1:2500

WB

1/5000 - 1/40000

IF

1:2000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

5 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202293Certificate of AnalysisDec 28, 2023 Ab175850
ZJ23F1202292Certificate of AnalysisDec 28, 2023 Ab175850
ZJ23F1202291Certificate of AnalysisDec 28, 2023 Ab175850
ZJ23F1202290Certificate of AnalysisDec 28, 2023 Ab175850
ZJ23R1200203Certificate of AnalysisDec 21, 2023 Ab175850

Related Documents

References

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