mAb (2D10); Mouse anti Human beta actin Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab155822-10μl (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab155822-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $201.90 | |
| Ab155822-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $300.90 | |
| Ab155822-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,500.90 |
mAb (2D10); Mouse anti Human beta actin Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
| Product Name | beta Actin Mouse mAb - Primary antibody, for WB,Flow,ICC/IF,ELISA various applications, from Human, generated in Mouse, specific to beta actin, IgG1 |
|---|---|
| Synonyms | A26C1A antibody; A26C1B antibody; ACTB antibody; ACTB_HUMAN antibody; Actin beta antibody; Actin cytoplasmic 1 antibody; Actin, cytoplasmic 1, N-terminally processed antibody; Actx antibody; b actin antibody; b-actin antibody; β actin antibody; β-actin an |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by lot |
| Host Species | Mouse |
| Host species | Mouse |
| Specificity | beta actin |
| Application | WB,Flow,ICC/IF,ELISA |
| Immunogen | Synthetic peptide in beta Actin |
| Positive Control | WB: A549, MCF-7, HeLa, Hep G2, Raji, Ramos, MOLT-4, Jurkat, LNCap, A431, HEK293, NIH/3T3, K562, HT-29, THP-1 lysates. ICC/IF: HeLa cells. Flow: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Application： |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Monoclonal antibodies |
| Isotype | IgG1 |
| Light Chain Type | kappa |
| Purification Method | Protein A purified |
| Source | Hybridoma supernatant |
| Form | Liquid |
| Concentration | Lot by lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon delivery aliquot. Avoid freeze/thaw cycle. |
beta Actin Mouse mAb (Ab155822) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
beta Actin Mouse mAb (Ab155822) - ICC/IF
IF analysis of beta Actin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
beta Actin Mouse mAb (Ab155822) - Flow Cytometry
Flow analysis of beta Actin (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| ICC/IF |
0.5 μg/mL
|
| Flow Cytometry |
0.016-2.0μg/mL
|
| WB |
1/1,000-1/20,000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404950 | Certificate of Analysis | Apr 26, 2024 | Ab155822 |
| ZJ24F0404949 | Certificate of Analysis | Apr 26, 2024 | Ab155822 |
| ZJ24F0404948 | Certificate of Analysis | Apr 26, 2024 | Ab155822 |
| ZJ24F0404947 | Certificate of Analysis | Apr 26, 2024 | Ab155822 |
| ZJ23R0900103 | Certificate of Analysis | Sep 19, 2023 | Ab155822 |
| ZJ23F0700608 | Certificate of Analysis | Jul 14, 2023 | Ab155822 |
| ZJ23F0700609 | Certificate of Analysis | Jul 14, 2023 | Ab155822 |
| 1. Wang RE, Hunt CR, Chen J, Taylor JS.. (2011) Biotinylated quercetin as an intrinsic photoaffinity proteomics probe for the identification of quercetin target proteins.. Bioorg Med Chem, 19 (16): (4710-4720). [PMID:21798748] |
| 2. Angelo LS, Maxwell DS, Wu JY, Sun D, Hawke DH, McCutcheon IE, Slopis JM, Peng Z, Bornmann WG, Kurzrock R.. (2013) Binding partners for curcumin in human schwannoma cells: biologic implications.. Bioorg Med Chem, 21 (4): (932-939). [PMID:23294827] |
| 3. Ohmori, H H, Toyama, S S and Toyama, S S.. (1992) Direct proof that the primary site of action of cytochalasin on cell motility processes is actin.. The Journal of cell biology, [PMID:1734024] |
| 4. Nakajima-Iijima, S S, Hamada, H H, Reddy, P P and Kakunaga, T T.. (1985) Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2994062] |
| 5. Ponte, P P, Ng, S Y SY, Engel, J J, Gunning, P P and Kedes, L L.. (1984) Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA.. Nucleic acids research, (10): [PMID:6322116] |
| 6. Hanukoglu, I I, Tanese, N N and Fuchs, E E.. (1983) Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of 3' non-coding regions.. Journal of molecular biology, (5): [PMID:6842590] |
| 7. Zhang, Suisheng S and 5 more authors.. (2002) Nuclear DNA helicase II/RNA helicase A binds to filamentous actin.. The Journal of biological chemistry, (4): [PMID:11687588] |
| 8. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 9. Stüven, Theis T, Hartmann, Enno E and Görlich, Dirk D.. (2003) Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes.. The EMBO journal, (3): [PMID:14592989] |
| 10. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 11. Holaska, James M JM, Kowalski, Amy K AK and Wilson, Katherine L KL.. (2004) Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane.. PLoS biology, [PMID:15328537] |
| 12. Giannakopoulos, Nadia V NV and 9 more authors.. (2005) Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.. Biochemical and biophysical research communications, (21): [PMID:16139798] |
| 13. Procaccio, Vincent V and 14 more authors.. (2006) A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.. American journal of human genetics, [PMID:16685646] |
| 14. Jønson, Lars L and 8 more authors.. (2007) Molecular composition of IMP1 ribonucleoprotein granules.. Molecular & cellular proteomics : MCP, [PMID:17289661] |
| 15. Lu, Xiaoqing X and 5 more authors.. (2007) HGAL, a lymphoma prognostic biomarker, interacts with the cytoskeleton and mediates the effects of IL-6 on cell migration.. Blood, (15): [PMID:17823310] |
| 16. Lange, Martin M and 16 more authors.. (2008) Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex.. Genes & development, (1): [PMID:18765789] |
| 17. Kudryashov, Dmitri S DS and 10 more authors.. (2008) Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin.. Proceedings of the National Academy of Sciences of the United States of America, (25): [PMID:19015515] |
| 18. Li, Long-Yuan LY and 14 more authors.. (2011) Nuclear ErbB2 enhances translation and cell growth by activating transcription of ribosomal RNA genes.. Cancer research, (15): [PMID:21555369] |
| 19. Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17): [PMID:21838287] |
| 20. Schmidt, Mathias V MV and 19 more authors.. (2011) Tumor suppressor down-regulated in renal cell carcinoma 1 (DRR1) is a stress-induced actin bundling factor that modulates synaptic efficacy and cognition.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:21969592] |
| 21. Rivière, Jean-Baptiste JB and 32 more authors.. (2012) De novo mutations in the actin genes ACTB and ACTG1 cause Baraitser-Winter syndrome.. Nature genetics, (26): [PMID:22366783] |
| 22. Li, Ming-Ming MM and 18 more authors.. (2013) ALKBH4-dependent demethylation of actin regulates actomyosin dynamics.. Nature communications, [PMID:23673617] |
| 23. Heisler, David B and 10 more authors.. (2015) ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization.. Science (New York, N.Y.), (31): [PMID:26228148] |
| 24. Mu, P P, Akashi, T T, Lu, F F, Kishida, S S and Kadomatsu, K K.. (2017) A novel nuclear complex of DRR1, F-actin and COMMD1 involved in NF-κB degradation and cell growth suppression in neuroblastoma.. Oncogene, (12): [PMID:28604741] |
| 25. Drazic, Adrian A and 12 more authors.. (2018) NAA80 is actin's N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility.. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:29581253] |
| 26. Schrank, Benjamin R BR and 7 more authors.. (2018) Nuclear ARP2/3 drives DNA break clustering for homology-directed repair.. Nature, [PMID:29925947] |
| 27. Wiame, Elsa E and 6 more authors.. (2018) NAT6 acetylates the N-terminus of different forms of actin.. The FEBS journal, [PMID:30028079] |
| 28. Kwiatkowski, Sebastian S and 10 more authors.. (2018) SETD3 protein is the actin-specific histidine N-methyltransferase.. eLife, (11): [PMID:30526847] |
| 29. Wilkinson, Alex W AW and 21 more authors.. (2019) SETD3 is an actin histidine methyltransferase that prevents primary dystocia.. Nature, [PMID:30626964] |
| 30. Guo, Qiong Q and 9 more authors.. (2019) Structural insights into SETD3-mediated histidine methylation on β-actin.. eLife, (20): [PMID:30785395] |
