| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab155822-10μl (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μl | In stock | $66.90 | |
| Ab155822-50μl | 50μl | In stock | $201.90 | |
| Ab155822-100μl | 100μl | In stock | $300.90 | |
| Ab155822-1ml | 1ml | In stock | $1,500.90 |
mAb (2D10); Mouse anti Human beta actin Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
| Product Name | beta Actin Mouse mAb - Primary antibody, for WB,Flow,ICC/IF,ELISA various applications, from Human, generated in Mouse, specific to beta actin, IgG1 |
|---|---|
| Synonyms | A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | Beta cytoskeletal |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by lot |
| Host Species | Mouse |
| Host species | Mouse |
| Specificity | beta actin |
| Application | WB,Flow,ICC/IF,ELISA |
| Immunogen | Synthetic peptide in beta Actin |
| Positive Control | WB: A549, MCF-7, HeLa, Hep G2, Raji, Ramos, MOLT-4, Jurkat, LNCap, A431, HEK293, NIH/3T3, K562, HT-29, THP-1 lysates. ICC/IF: HeLa cells. Flow: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Note | 20μl卖完停产，不再备货 |
| Product Description |
Application： |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Monoclonal antibodies |
| Isotype | IgG1 |
| Light Chain Type | kappa |
| Purification Method | Protein A purified |
| Source | Hybridoma supernatant |
| Form | Liquid |
| Concentration | Lot by lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon delivery aliquot. Avoid freeze/thaw cycle. |
beta Actin Mouse mAb (Ab155822) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
beta Actin Mouse mAb (Ab155822) - ICC/IF
IF analysis of beta Actin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
beta Actin Mouse mAb (Ab155822) - Flow Cytometry
Flow analysis of beta Actin (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| IF/ICC |
0.5 μg/mL
|
| Flow Cytometry |
0.016-2.0μg/mL
|
| WB |
1/1,000-1/20,000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404950 | Certificate of Analysis | Apr 26, 2024 | Ab155822 |
| ZJ24F0404949 | Certificate of Analysis | Apr 26, 2024 | Ab155822 |
| ZJ24F0404948 | Certificate of Analysis | Apr 26, 2024 | Ab155822 |
| ZJ24F0404947 | Certificate of Analysis | Apr 26, 2024 | Ab155822 |
| ZJ23R0900103 | Certificate of Analysis | Sep 19, 2023 | Ab155822 |
| ZJ23F0700608 | Certificate of Analysis | Jul 14, 2023 | Ab155822 |
| ZJ23F0700609 | Certificate of Analysis | Jul 14, 2023 | Ab155822 |
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