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beta Actin Mouse mAb (HRP) - Primary antibody, specific to beta actin, IgG1

  • Azide Free
  • ExactAb™
  • High performance
  • Validated
  • 0.5 mg/mL
Features and benefits
  • Short Overview:

    mAb ( FG01/2D10); Mouse anti Human beta actin Antibody; WB; HRP

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: IgG1
    Application:
  • WB
Item Number
Ab175849
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab175849-10μl
10μl
In stock
$66.90
Ab175849-20μl
20μl
In stock
$99.90
Ab175849-50μl
50μl
In stock
$199.90
Ab175849-100μl
100μl
In stock
$299.90
Ab175849-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,499.90

mAb ( FG01/2D10); Mouse anti Human beta actin Antibody; WB; HRP

Basic Description

Product Namebeta Actin Mouse mAb (HRP) - Primary antibody, specific to beta actin, IgG1
SynonymsA26C1A antibody|A26C1B antibody|ACTB antibody|ACTB_HUMAN antibody|Actin beta antibody|Actin cytoplasmic 1 antibody|Actin, cytoplasmic 1, N-terminally processed antibody|Actx antibody|b actin antibody|b-actin antibody|Beta cytoskeletal actin antibody|Beta-
Specifications & PurityExactAb™, Validated, Azide Free, High performance, 0.5 mg/mL
Host speciesMouse
Specificitybeta actin
ImmunogenSynthetic peptide in beta Actin
Positive ControlWB: K562, A549, HeLa, Jurkat, NIH3T3 and C6 whole cell lysates
ConjugationHRP
GradeAzide Free, ExactAb™, High performance, Validated
Product Description

Application:
WB :1:2000-1:10000

Product Properties

IsotypeIgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.5 mg/mL
Storage TempProtected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

beta Actin Mouse mAb (HRP) (Ab175849) - Western Blot
All lanes: beta Actin Mouse mAb (HRP) (Ab175849) at 1/5000 dilution
Samples: Lysates at 20 µg per lane

Predicted band size: 42.0 kDa
Observed band size: 42.0 kDa
Exposure time: 41.8s

Associated Targets

ACTB Tbio Actin, cytoplasmic 1 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/2000 - 1/10000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

5 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202289Certificate of AnalysisDec 28, 2023 Ab175849
ZJ23F1202288Certificate of AnalysisDec 28, 2023 Ab175849
ZJ23F1202287Certificate of AnalysisDec 28, 2023 Ab175849
ZJ23F1202286Certificate of AnalysisDec 28, 2023 Ab175849
ZJ23R1200202Certificate of AnalysisDec 21, 2023 Ab175849

Related Documents

References

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15. Lu, Xiaoqing X and 5 more authors..  (2007)  HGAL, a lymphoma prognostic biomarker, interacts with the cytoskeleton and mediates the effects of IL-6 on cell migration..  Blood,    (15):   [PMID:17823310]
16. Lange, Martin M and 16 more authors..  (2008)  Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex..  Genes & development,    (1):   [PMID:18765789]
17. Kudryashov, Dmitri S DS and 10 more authors..  (2008)  Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin..  Proceedings of the National Academy of Sciences of the United States of America,    (25):   [PMID:19015515]
18. Li, Long-Yuan LY and 14 more authors..  (2011)  Nuclear ErbB2 enhances translation and cell growth by activating transcription of ribosomal RNA genes..  Cancer research,    (15):   [PMID:21555369]
19. Mi, Lixin and 8 more authors..  (2011)  Identification of potential protein targets of isothiocyanates by proteomics..  Chemical research in toxicology,    (17):   [PMID:21838287]
20. Schmidt, Mathias V MV and 19 more authors..  (2011)  Tumor suppressor down-regulated in renal cell carcinoma 1 (DRR1) is a stress-induced actin bundling factor that modulates synaptic efficacy and cognition..  Proceedings of the National Academy of Sciences of the United States of America,    (11):   [PMID:21969592]
21. Rivière, Jean-Baptiste JB and 32 more authors..  (2012)  De novo mutations in the actin genes ACTB and ACTG1 cause Baraitser-Winter syndrome..  Nature genetics,    (26):   [PMID:22366783]
22. Li, Ming-Ming MM and 18 more authors..  (2013)  ALKBH4-dependent demethylation of actin regulates actomyosin dynamics..  Nature communications,      [PMID:23673617]
23. Heisler, David B and 10 more authors..  (2015)  ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization..  Science (New York, N.Y.),    (31):   [PMID:26228148]
24. Mu, P P, Akashi, T T, Lu, F F, Kishida, S S and Kadomatsu, K K..  (2017)  A novel nuclear complex of DRR1, F-actin and COMMD1 involved in NF-κB degradation and cell growth suppression in neuroblastoma..  Oncogene,    (12):   [PMID:28604741]
25. Drazic, Adrian A and 12 more authors..  (2018)  NAA80 is actin's N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility..  Proceedings of the National Academy of Sciences of the United States of America,    (24):   [PMID:29581253]
26. Schrank, Benjamin R BR and 7 more authors..  (2018)  Nuclear ARP2/3 drives DNA break clustering for homology-directed repair..  Nature,      [PMID:29925947]
27. Wiame, Elsa E and 6 more authors..  (2018)  NAT6 acetylates the N-terminus of different forms of actin..  The FEBS journal,      [PMID:30028079]
28. Kwiatkowski, Sebastian S and 10 more authors..  (2018)  SETD3 protein is the actin-specific histidine N-methyltransferase..  eLife,    (11):   [PMID:30526847]
29. Wilkinson, Alex W AW and 21 more authors..  (2019)  SETD3 is an actin histidine methyltransferase that prevents primary dystocia..  Nature,      [PMID:30626964]
30. Guo, Qiong Q and 9 more authors..  (2019)  Structural insights into SETD3-mediated histidine methylation on β-actin..  eLife,    (20):   [PMID:30785395]

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