| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab175849-10μl | 10μl | In stock | $66.90 | |
| Ab175849-20μl | 20μl | In stock | $99.90 | |
| Ab175849-50μl | 50μl | In stock | $199.90 | |
| Ab175849-100μl | 100μl | In stock | $299.90 | |
| Ab175849-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,499.90 |
mAb ( FG01/2D10); Mouse anti Human beta actin Antibody; WB; HRP
| Product Name | beta Actin Mouse mAb (HRP) - Primary antibody, specific to beta actin, IgG1 |
|---|---|
| Synonyms | A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | Beta cytoskeletal |
| Specifications & Purity | ExactAb™, Validated, Azide Free, High performance, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | beta actin |
| Immunogen | Synthetic peptide in beta Actin |
| Positive Control | WB: K562, A549, HeLa, Jurkat, NIH3T3 and C6 whole cell lysates |
| Conjugation | HRP |
| Grade | Azide Free, ExactAb™, High Performance, Validated |
| Product Description |
Application: |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Protected from light,Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light. |
beta Actin Mouse mAb (HRP) (Ab175849) - Western Blot
All lanes: beta Actin Mouse mAb (HRP) (Ab175849) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Predicted band size: 42.0 kDa
Observed band size: 42.0 kDa
Exposure time: 41.8s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/2000 - 1/10000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202289 | Certificate of Analysis | Dec 28, 2023 | Ab175849 |
| ZJ23F1202288 | Certificate of Analysis | Dec 28, 2023 | Ab175849 |
| ZJ23F1202287 | Certificate of Analysis | Dec 28, 2023 | Ab175849 |
| ZJ23F1202286 | Certificate of Analysis | Dec 28, 2023 | Ab175849 |
| ZJ23R1200202 | Certificate of Analysis | Dec 21, 2023 | Ab175849 |
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| 7. Zhang, Suisheng S and 5 more authors.. (2002) Nuclear DNA helicase II/RNA helicase A binds to filamentous actin.. The Journal of biological chemistry, (4): [PMID:11687588] |
| 8. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 9. Stüven, Theis T, Hartmann, Enno E and Görlich, Dirk D.. (2003) Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes.. The EMBO journal, (3): [PMID:14592989] |
| 10. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 11. Holaska, James M JM, Kowalski, Amy K AK and Wilson, Katherine L KL.. (2004) Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane.. PLoS biology, [PMID:15328537] |
| 12. Giannakopoulos, Nadia V NV and 9 more authors.. (2005) Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.. Biochemical and biophysical research communications, (21): [PMID:16139798] |
| 13. Procaccio, Vincent V and 14 more authors.. (2006) A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.. American journal of human genetics, [PMID:16685646] |
| 14. Jønson, Lars L and 8 more authors.. (2007) Molecular composition of IMP1 ribonucleoprotein granules.. Molecular & cellular proteomics : MCP, [PMID:17289661] |
| 15. Lu, Xiaoqing X and 5 more authors.. (2007) HGAL, a lymphoma prognostic biomarker, interacts with the cytoskeleton and mediates the effects of IL-6 on cell migration.. Blood, (15): [PMID:17823310] |
| 16. Lange, Martin M and 16 more authors.. (2008) Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex.. Genes & development, (1): [PMID:18765789] |
| 17. Kudryashov, Dmitri S DS and 10 more authors.. (2008) Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin.. Proceedings of the National Academy of Sciences of the United States of America, (25): [PMID:19015515] |
| 18. Li, Long-Yuan LY and 14 more authors.. (2011) Nuclear ErbB2 enhances translation and cell growth by activating transcription of ribosomal RNA genes.. Cancer research, (15): [PMID:21555369] |
| 19. Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17): [PMID:21838287] |
| 20. Schmidt, Mathias V MV and 19 more authors.. (2011) Tumor suppressor down-regulated in renal cell carcinoma 1 (DRR1) is a stress-induced actin bundling factor that modulates synaptic efficacy and cognition.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:21969592] |
| 21. Rivière, Jean-Baptiste JB and 32 more authors.. (2012) De novo mutations in the actin genes ACTB and ACTG1 cause Baraitser-Winter syndrome.. Nature genetics, (26): [PMID:22366783] |
| 22. Li, Ming-Ming MM and 18 more authors.. (2013) ALKBH4-dependent demethylation of actin regulates actomyosin dynamics.. Nature communications, [PMID:23673617] |
| 23. Heisler, David B and 10 more authors.. (2015) ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization.. Science (New York, N.Y.), (31): [PMID:26228148] |
| 24. Mu, P P, Akashi, T T, Lu, F F, Kishida, S S and Kadomatsu, K K.. (2017) A novel nuclear complex of DRR1, F-actin and COMMD1 involved in NF-κB degradation and cell growth suppression in neuroblastoma.. Oncogene, (12): [PMID:28604741] |
| 25. Drazic, Adrian A and 12 more authors.. (2018) NAA80 is actin's N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility.. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:29581253] |
| 26. Schrank, Benjamin R BR and 7 more authors.. (2018) Nuclear ARP2/3 drives DNA break clustering for homology-directed repair.. Nature, [PMID:29925947] |
| 27. Wiame, Elsa E and 6 more authors.. (2018) NAT6 acetylates the N-terminus of different forms of actin.. The FEBS journal, [PMID:30028079] |
| 28. Kwiatkowski, Sebastian S and 10 more authors.. (2018) SETD3 protein is the actin-specific histidine N-methyltransferase.. eLife, (11): [PMID:30526847] |
| 29. Wilkinson, Alex W AW and 21 more authors.. (2019) SETD3 is an actin histidine methyltransferase that prevents primary dystocia.. Nature, [PMID:30626964] |
| 30. Guo, Qiong Q and 9 more authors.. (2019) Structural insights into SETD3-mediated histidine methylation on β-actin.. eLife, (20): [PMID:30785395] |
