| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab177877-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $109.90 | |
| Ab177877-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $519.90 | |
| Ab177877-5mg | 5mg | In stock | $1,399.90 | |
| Ab177877-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,239.90 |
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<0.01EU/μg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| Product Name | BHQ880 (anti-DKK1) - Primary antibody, specific to DKK1, >95% (SDS-PAGE&SEC), high purity, Human IgG1 |
|---|---|
| Synonyms | Dickkopf 1 antibody; Dickkopf 1 homolog antibody; Dickkopf 1 like antibody; Dickkopf homolog 1 antibody; Dickkopf like protein 1 antibody; Dickkopf related protein 1 antibody; Dickkopf WNT signaling pathway inhibitor 1 antibody; Dickkopf-1 antibody; Dickk |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot |
| Host species | Human |
| Specificity | DKK1 |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Recombinant, Validated |
| Product Description | BHQ880 (anti-DKK1) is a humanized monoclonal antibody targeting Wnt antagonist Dickkopf-1 (DKK1). It binds to and inhibits DKK1, enhancing signaling through the Wnt pathway, which result in osteoblast differentiation and activation within the bone matrix and the reversal of tumor-induced osteolytic disease. |
| Isotype | Human IgG1 |
|---|---|
| Light Chain Type | lambda |
| SDS-PAGE | 27.2 kDa (Light Chain) & 49.6 kDa (Heavy Chain), under reducing conditions; 190.3 kDa, under non-reducing conditions. |
| Purification Method | Protein A purified |
| Purity | >95% (SDS-PAGE&SEC) |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
BHQ880 (anti-DKK1) (Ab177877) - Flow Cytometry
Flow Cytometry analysis of HepG2 cells labelling DKK1 (red) with BHQ880 (anti-DKK1) (Ab177877). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
BHQ880 (anti-DKK1) (Ab177877) - SEC
The purity of BHQ880 (anti-DKK1) (Ab177877) is more than 95% verified by HPLC.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
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Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404124 | Certificate of Analysis | Apr 03, 2024 | Ab177877 |
| ZJ24F0404123 | Certificate of Analysis | Apr 03, 2024 | Ab177877 |
| ZJ24F0404122 | Certificate of Analysis | Apr 03, 2024 | Ab177877 |
| 1. Mpousis S, Thysiadis S, Avramidis N, Katsamakas S, Efthimiopoulos S, Sarli V.. (2016) Synthesis and evaluation of gallocyanine dyes as potential agents for the treatment of Alzheimer's disease and related neurodegenerative tauopathies.. Eur J Med Chem, 108 (28-38). [PMID:26629858] [10.1016/j.ejmech.2015.11.024] |
| 2. Fedi, P P and 7 more authors.. (1999) Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling.. The Journal of biological chemistry, (2): [PMID:10383463] |
| 3. Krupnik, V E VE and 15 more authors.. (1999) Functional and structural diversity of the human Dickkopf gene family.. Gene, (1): [PMID:10570958] |
| 4. Roessler, E E and 5 more authors.. (2000) The genomic structure, chromosome location, and analysis of the human DKK1 head inducer gene as a candidate for holoprosencephaly.. Cytogenetics and cell genetics, [PMID:10965128] |
| 5. Semënov, M V MV and 5 more authors.. (2001) Head inducer Dickkopf-1 is a ligand for Wnt coreceptor LRP6.. Current biology : CB, (26): [PMID:11448771] |
| 6. and Niehrs, C C.. (2006) Function and biological roles of the Dickkopf family of Wnt modulators.. Oncogene, (4): [PMID:17143291] |
| 7. Binnerts, Minke E ME and 18 more authors.. (2007) R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:17804805] |
| 8. Murrills, Richard J RJ and 9 more authors.. (2009) A cell-based Dkk1 binding assay reveals roles for extracellular domains of LRP5 in Dkk1 interaction and highlights differences between wild-type and the high bone mass mutant LRP5(G171V).. Journal of cellular biochemistry, (1): [PMID:19746449] |
| 9. Bourhis, Eric E and 8 more authors.. (2010) Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6.. The Journal of biological chemistry, (19): [PMID:20093360] |
| 10. Haniu, Mitsuru M and 7 more authors.. (2011) Human Dickkopf-1 (huDKK1) protein: characterization of glycosylation and determination of disulfide linkages in the two cysteine-rich domains.. Protein science : a publication of the Protein Society, [PMID:21805521] |
| 11. Ahn, Victoria E VE and 5 more authors.. (2011) Structural basis of Wnt signaling inhibition by Dickkopf binding to LRP5/6.. Developmental cell, (15): [PMID:22000856] |
| 12. Zebisch, Matthias M, Jackson, Verity A VA, Zhao, Yuguang Y and Jones, E Yvonne EY.. (2016) Structure of the Dual-Mode Wnt Regulator Kremen1 and Insight into Ternary Complex Formation with LRP6 and Dickkopf.. Structure (London, England : 1993), (6): [PMID:27524201] |
