BIS-TRIS propane - 99%, high purity , CAS No.64431-96-5

Item Number

B105640

• Synonyms: 1,3-Bis[tris(hydroxymethyl)methylamino]propane | Bis-Tris propane | bis(2-(hydroxymethyl)propane-1,3-diol) | 2,2'-(Propane-1,3-diylbis(azanediyl))
• Specifications & Purity: ≥99%
• Biochemical and Physiological Mechanisms: Buffer that is useful in the pH 6.3-9.5 range. This wide buffering range is due to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being so close.
• Storage Temp: Argon charged
• Shipped In: Normal
• Note: Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Product Description

Bis-Tris Propane buffer, also known as BTP, is a zwitterionic biochemical buffer utilized for pH regulation in various laboratory applications, particularly in molecular biology, biochemistry, and electrophoresis. Its wide buffering range, spanning from pH 6.3 to 9.5, is attributed to its two pKa values of 6.8 (pKa1) and 9.0 (pKa2), which are close. This wide range makes it highly versatile for maintaining stable pH conditions in a variety of experiments. Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III).

Product Application：
BIS-TRIS Propane may be used in the following studies: as a buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO2). for purification of glucose-binding protein from membranes of Sulfolobus solfataricus. as a buffer during GeO2 mineralisation. as a buffer component in a study pertaining to environmental research as a buffer component to maintain optimal pH conditions during the purification and preparation of engineered enzymes