| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab091608-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab091608-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $199.90 | |
| Ab091608-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $309.90 | |
| Ab091608-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,059.90 |
mAb (125CT13.3.5); Mouse anti Human BRAF Antibody; WB, ICC, IF; Unconjugated
| Product Name | BRAF Mouse mAb - Primary antibody, specific to BRAF, Mouse IgG1 |
|---|---|
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | BRAF |
| Immunogen | BRAF recombinant protein (AA 4-365). |
| Positive Control | WB: MCF-7, K562, HeLa, Hep G2 and HEK293 cell lysates. ICC/IF: C2C12 cells |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human BRAF Antibody, Monoclonal (125CT13.3.5), could be used for WB, IF, ICC and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
BRAF Mouse mAb (Ab091608) - Western Blot
All lanes: BRAF Mouse mAb (Ab091608) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 85 kDa
Exposure time: 17.9 s
BRAF Mouse mAb (Ab091608) - ICC/IF
Fluorescent image of C2C12 cells stained with BRAF Antibody (Ab091608). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody 1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used 1:400, 50 min at 37℃. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin 7 units/mL, 1 h at 37℃. BRAF immunoreactivity is localized to Cytoplasm significantly.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
| IF/ICC |
1/10-1/50
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800966 | Certificate of Analysis | Sep 04, 2023 | Ab091608 |
| ZJ23F0800965 | Certificate of Analysis | Sep 04, 2023 | Ab091608 |
