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BRG1 Mouse mAb - Primary antibody, specific to BRG1, Mouse IgG1

ExactAb™
Validated
Lot by Lot

Features and benefits

Short Overview:
mAb (6D7-F7-B6); Mouse anti Human BRG1 Antibody; WB, ICC, IF, IP; Unconjugated
Species reactivity(Reacts with): Human,Mouse
Isotype: Mouse IgG1

Application:

ICC/IF
IP
WB

Item Number

Ab091716

Grouped product items
SKU	Size	Availability	Price
Ab091716-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab091716-50μl	50μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$209.90
Ab091716-100μl	100μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$319.90
Ab091716-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,699.90

mAb (6D7-F7-B6); Mouse anti Human BRG1 Antibody; WB, ICC, IF, IP; Unconjugated

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Accession#:P51532 BRG1 Gene ID:6597 SMARCA4

Basic Description

Product Name	BRG1 Mouse mAb - Primary antibody, specific to BRG1, Mouse IgG1
Synonyms	ATP-dependent helicase SMARCA4; BAF190; BAF190A; brahma protein-like 1; Brg1; BRG1-associated factor 190A; BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4; BRM/SWI2-related gene 1; EC 3.6.1; EC 3.6.4.-; FLJ
Specifications & Purity	ExactAb™, Validated, Lot by Lot
Host species	Mouse
Specificity	BRG1
Immunogen	Purified recombinant human BRG1 protein fragments expressed in E.coli (AA 205-300).
Positive Control	WB: HeLa, Raw264.7, K562, Jurkat and Hep G2 cell lysates. ICC/IF: HeLa cells. IP: K562 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Validated
Product Description	Mouse anti Human BRG1 Antibody, Monoclonal (6D7-F7-B6), could be used for WB, ICC, IF, IP and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 IP: 1/20 Protein Function Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

BRG1 Mouse mAb (Ab091716) - Western Blot
All lanes: BRG1 Mouse mAb (Ab091716) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 220 kDa
Observed band size: 213 kDa
Exposure time: 61.7 s

BRG1 Mouse mAb (Ab091716) - IF
Immunocytochemistry analysis of BRG1 (green) in HeLa cells using BRG1 Mouse mAb (Ab091716) and cells were counterstained with DAPI (blue).

BRG1 Mouse mAb (Ab091716) - IP
Immunoprecipitation analysis of BRG1 in K562 cell lysates using BRG1 antibody.

Associated Targets

SMARCA4 Tchem Transcription activator BRG1 0 Activities

Discover Target: SMARCA4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000
ICC/IF	1/50-1/200
IP	1/20

Certificates

Certificate of Analysis(COA)

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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0901059	Certificate of Analysis	Sep 14, 2023	Ab091716
ZJ23F0901058	Certificate of Analysis	Sep 14, 2023	Ab091716

FAQs and Articles

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Categories: Technical articles

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Immunoprecipitation (IP) lysates and reagents

Categories: Protocols

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Immunoprecipitation lysate Tissue lysate

References

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19. Zhao Y, Bai L, Liu L, McEachern D, Stuckey JA, Meagher JL, Yang CY, Ran X, Zhou B, Hu Y, Li X, Wen B, Zhao T, Li S, Sun D, Wang S.. (2017) Structure-Based Discovery of 4-(6-Methoxy-2-methyl-4-(quinolin-4-yl)-9H-pyrimido[4,5-b]indol-7-yl)-3,5-dimethylisoxazole (CD161) as a Potent and Orally Bioavailable BET Bromodomain Inhibitor.. J Med Chem, 60 (9): (3887-3901). [PMID:28463487]
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24. Chiba, H H, Muramatsu, M M, Nomoto, A A and Kato, H H.. (1994) Two human homologues of Saccharomyces cerevisiae SWI2/SNF2 and Drosophila brahma are transcriptional coactivators cooperating with the estrogen receptor and the retinoic acid receptor.. Nucleic acids research, (25): [PMID:8208605]
25. Khavari, P A PA, Peterson, C L CL, Tamkun, J W JW, Mendel, D B DB and Crabtree, G R GR.. (1993) BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription.. Nature, (11): [PMID:8232556]
26. Fryer, C J CJ and Archer, T K TK.. (1998) Chromatin remodelling by the glucocorticoid receptor requires the BRG1 complex.. Nature, (7): [PMID:9590696]
27. Kim, J J and 11 more authors.. (1999) Ikaros DNA-binding proteins direct formation of chromatin remodeling complexes in lymphocytes.. Immunity, [PMID:10204490]
28. Wong, A K AK and 18 more authors.. (2000) BRG1, a component of the SWI-SNF complex, is mutated in multiple human tumor cell lines.. Cancer research, (1): [PMID:11085541]
29. Park, Jeonghyeon J, Wood, Marcelo A MA and Cole, Michael D MD.. (2002) BAF53 forms distinct nuclear complexes and functions as a critical c-Myc-interacting nuclear cofactor for oncogenic transformation.. Molecular and cellular biology, [PMID:11839798]
30. Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1): [PMID:15057824]
31. Liu, Kang K, Luo, Yuhong Y, Lin, Fang-Tsyr FT and Lin, Weei-Chin WC.. (2004) TopBP1 recruits Brg1/Brm to repress E2F1-induced apoptosis, a novel pRb-independent and E2F1-specific control for cell survival.. Genes & development, (15): [PMID:15075294]
32. Huang, Chun-Yin CY and 7 more authors.. (2005) hZimp7, a novel PIAS-like protein, enhances androgen receptor-mediated transcription and interacts with SWI/SNF-like BAF complexes.. Molecular endocrinology (Baltimore, Md.), [PMID:16051670]
33. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243]
34. Shen, Weiqun W and 7 more authors.. (2007) Solution structure of human Brg1 bromodomain and its specific binding to acetylated histone tails.. Biochemistry, (27): [PMID:17274598]
35. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332]
36. Singh, Mahavir M, Popowicz, Grzegorz M GM, Krajewski, Marcin M and Holak, Tad A TA.. (2007) Structural ramification for acetyl-lysine recognition by the bromodomain of human BRG1 protein, a central ATPase of the SWI/SNF remodeling complex.. Chembiochem : a European journal of chemical biology, (23): [PMID:17582821]
37. Medina, Pedro P PP and 6 more authors.. (2008) Frequent BRG1/SMARCA4-inactivating mutations in human lung cancer cell lines.. Human mutation, [PMID:18386774]
38. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
39. Lange, Martin M and 16 more authors.. (2008) Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex.. Genes & development, (1): [PMID:18765789]
40. Park, Jae-Il JI and 14 more authors.. (2009) Telomerase modulates Wnt signalling by association with target gene chromatin.. Nature, (2): [PMID:19571879]
41. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
42. Schneppenheim, Reinhard R and 12 more authors.. (2010) Germline nonsense mutation and somatic inactivation of SMARCA4/BRG1 in a family with rhabdoid tumor predisposition syndrome.. American journal of human genetics, (12): [PMID:20137775]
43. Sánchez-Tilló, E E and 7 more authors.. (2010) ZEB1 represses E-cadherin and induces an EMT by recruiting the SWI/SNF chromatin-remodeling protein BRG1.. Oncogene, (17): [PMID:20418909]
44. Tando, Toshio T and 11 more authors.. (2010) Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway.. The Journal of biological chemistry, (16): [PMID:20460684]
45. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
46. Tsurusaki, Yoshinori Y and 30 more authors.. (2012) Mutations affecting components of the SWI/SNF complex cause Coffin-Siris syndrome.. Nature genetics, (18): [PMID:22426308]
47. Euskirchen, Ghia G, Auerbach, Raymond K RK and Snyder, Michael M.. (2012) SWI/SNF chromatin-remodeling factors: multiscale analyses and diverse functions.. The Journal of biological chemistry, (7): [PMID:22952240]
48. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
49. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569]
50. Gong, Fade F and 11 more authors.. (2015) Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination.. Genes & development, (15): [PMID:25593309]
51. Marom, Ronit R and 23 more authors.. (2017) Heterozygous variants in ACTL6A, encoding a component of the BAF complex, are associated with intellectual disability.. Human mutation, [PMID:28649782]
52. Alpsoy, Aktan A and Dykhuizen, Emily C EC.. (2018) Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes.. The Journal of biological chemistry, (16): [PMID:29374058]

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