| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab175482-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $79.90 | |
| Ab175482-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $389.90 | |
| Ab175482-5mg | 5mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $999.90 | |
| Ab175482-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,599.90 |
Purity≥95% (SDS-PAGE&SEC); Endotoxin Level≤1.0EU/mg; Human IgG2SA; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| Product Name | Brontictuzumab (anti-NOTCH1) - Primary antibody, specific to NOTCH1, >95%, high purity, Human IgG2 |
|---|---|
| Synonyms | 9930111A19Rik antibody | AOS5 antibody | AOVD1 antibody | hN1 antibody | Lin-12 antibody | LIN12 antibody | Mis6 antibody | Motch A antibody | mT14 antibody | Neurogenic locus notch homolog protein 1 antibody | Neurogenic locus notch protein homolog antib |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot |
| Host species | Human |
| Specificity | NOTCH1 |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated |
| Action Type | INHIBITOR |
| Mechanism of action | Antibody of notch receptor 1 |
| Product Description |
来源MCE: HY-P99258；P230003402批次浓度为 8.13 mg/mL.
|
| Isotype | Human IgG2SA |
|---|---|
| Light Chain Type | lambdaC2 |
| SDS-PAGE | 27.8 kDa (Light Chain) & 54.5 kDa (Heavy Chain), under reducing conditions; 188.9 kDa, under non-reducing conditions. |
| Purification Method | Protein A purified |
| Purity | >95% |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| CAS | 1447814-75-6 |
Brontictuzumab (anti-NOTCH1) (Ab175482) - Flow Cytometry
Flow Cytometry analysis of MOLT-4 cells labelling NOTCH1 (red) with Brontictuzumab (anti-NOTCH1) (Ab175482). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
Brontictuzumab (anti-NOTCH1) (Ab175482) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling NOTCH1 (red) with Brontictuzumab (anti-NOTCH1) (Ab175482). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
Brontictuzumab (anti-NOTCH1) (Ab175482) - SEC
The purity of Brontictuzumab (anti-NOTCH1) (Ab175482) is more than 95% verified by HPLC.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| IMGT/mAb-DB | 492 |
|---|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505429 | Certificate of Analysis | May 11, 2024 | Ab175482 |
| ZJ24F0505428 | Certificate of Analysis | May 11, 2024 | Ab175482 |
| ZJ24F0505427 | Certificate of Analysis | May 11, 2024 | Ab175482 |
| 1. Ferrarotto R, Mitani Y, Diao L, Guijarro I, Wang J, Zweidler-McKay P, Bell D, William Jr WN, Glisson BS, Wick MJ et al.. (2017) Activating NOTCH1 Mutations Define a Distinct Subgroup of Patients With Adenoid Cystic Carcinoma Who Have Poor Prognosis, Propensity to Bone and Liver Metastasis, and Potential Responsiveness to Notch1 Inhibitors.. J Clin Oncol, 35 (3): (352-360). [PMID:27870570] [10.1021/op500134e] |
| 2. López-Guerra M, Xargay-Torrent S, Fuentes P, Roldán J, González-Farré B, Rosich L, Silkenstedt E, García-León MJ, Lee-Vergés E, Giménez N et al.. (2020) Specific NOTCH1 antibody targets DLL4-induced proliferation, migration, and angiogenesis in NOTCH1-mutated CLL cells.. Oncogene, 39 (6): (1185-1197). [PMID:31616059] [10.1021/op500134e] |
| 3. Silkenstedt E, Arenas F, Colom-Sanmartí B, Xargay-Torrent S, Higashi M, Giró A, Rodriguez V, Fuentes P, Aulitzky WE, van der Kuip H et al.. (2019) Notch1 signaling in NOTCH1-mutated mantle cell lymphoma depends on Delta-Like ligand 4 and is a potential target for specific antibody therapy.. J Exp Clin Cancer Res, 38 (1): (446). [PMID:31676012] [10.1021/op500134e] |
| 4. Nam Y, Sliz P, Song L, Aster JC, Blacklow SC. (2006) Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.. Cell, 124 (5): (973-83). [PMID:16530044] [10.1021/op500134e] |
| 5. Garg V, Muth AN, Ransom JF, Schluterman MK, Barnes R, King IN, Grossfeld PD, Srivastava D. (2005) Mutations in NOTCH1 cause aortic valve disease.. Nature, 437 (7056): (270-4). [PMID:16025100] [10.1021/op500134e] |
| 6. Stittrich AB, Lehman A, Bodian DL, Ashworth J, Zong Z, Li H, Lam P, Khromykh A, Iyer RK, Vockley JG et al.. (2014) Mutations in NOTCH1 cause Adams-Oliver syndrome.. Am J Hum Genet, 95 (3): (275-84). [PMID:25132448] [10.1021/op500134e] |
| 7. Astudillo L, Da Silva TG, Wang Z, Han X, Jin K, VanWye J, Zhu X, Weaver K, Oashi T, Lopes PE et al.. (2016) The Small Molecule IMR-1 Inhibits the Notch Transcriptional Activation Complex to Suppress Tumorigenesis.. Cancer Res, 76 (12): (3593-603). [PMID:27197169] [10.1021/op500134e] |
| 8. Flesch D, Ness J, Lamers C, Dehm F, Popella S, Steri R, Ogorek I, Hieke M, Dannhardt G, Werz O, Weggen S, Schubert-Zsilavecz M.. (2015) SAR-studies of γ-secretase modulators with PPARγ-agonistic and 5-lipoxygenase-inhibitory activity for Alzheimer's disease.. Bioorg Med Chem Lett, 25 (4): (841-846). [PMID:25575659] [10.1016/j.bmcl.2014.12.073] |
| 9. Shan W, Balog A, Quesnelle C, Gill P, Han WC, Norris D, Mandal S, Thiruvenkadam R, Gona KB, Thiyagarajan K, Kandula S, McGlinchey K, Menard K, Wen ML, Rose A, White R, Guarino V, Shen DR, Cvijic ME, Ranasinghe A, Dai J, Zhang Y, Wu DR, Mathur A, Rampulla R, Trainor G, Hunt JT, Vite GD, Westhouse R, Lee FY, Gavai AV.. (2015) BMS-871: a novel orally active pan-Notch inhibitor as an anticancer agent.. Bioorg Med Chem Lett, 25 (9): (1905-1909). [PMID:25857941] [10.1016/j.bmcl.2015.03.038] |
| 10. Yoneyama T, Arai MA, Akamine R, Koryudzu K, Tsuchiya A, Sadhu SK, Ahmed F, Itoh M, Okamoto R, Ishibashi M.. (2017) Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells.. J Nat Prod, 80 (9): (2453-2461). [PMID:28817274] [10.1021/acs.jnatprod.7b00282] |
| 11. Gómez-Galeno JE, Hurtado C, Cheng J, Yardimci C, Mercola M, Cashman JR.. (2018) b-Annulated 1,4-dihydropyridines as Notch inhibitors.. Bioorg Med Chem Lett, 28 (20): (3363-3367). [PMID:30201292] [10.1016/j.bmcl.2018.09.002] |
| 12. Quaglio D, Zhdanovskaya N, Tobajas G, Cuartas V, Balducci S, Christodoulou MS, Fabrizi G, Gargantilla M, Priego EM, Carmona Pestaña Á, Passarella D, Screpanti I, Botta B, Palermo R, Mori M, Ghirga F, Pérez-Pérez MJ.. (2019) Chalcones and Chalcone-mimetic Derivatives as Notch Inhibitors in a Model of T-cell Acute Lymphoblastic Leukemia.. ACS Med Chem Lett, 10 (4): (639-643). [PMID:30996810] [10.1021/acsmedchemlett.8b00608] |
| 13. Ellisen, L W LW and 6 more authors.. (1991) TAN-1, the human homolog of the Drosophila notch gene, is broken by chromosomal translocations in T lymphoblastic neoplasms.. Cell, (23): [PMID:1831692] |
| 14. Matsuno, K K and 7 more authors.. (1998) Human deltex is a conserved regulator of Notch signalling.. Nature genetics, [PMID:9590294] |
| 15. Gray, G E GE and 9 more authors.. (1999) Human ligands of the Notch receptor.. The American journal of pathology, [PMID:10079256] |
| 16. Zhou, S S and 6 more authors.. (2000) SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function.. Molecular and cellular biology, [PMID:10713164] |
| 17. Wu, L L and 5 more authors.. (2000) MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors.. Nature genetics, [PMID:11101851] |
| 18. Sakamoto, Kei K and 8 more authors.. (2002) The nephroblastoma overexpressed gene (NOV/ccn3) protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway.. The Journal of biological chemistry, (16): [PMID:12050162] |
| 19. Wu, Lizi L, Sun, Tao T, Kobayashi, Karla K, Gao, Ping P and Griffin, James D JD.. (2002) Identification of a family of mastermind-like transcriptional coactivators for mammalian notch receptors.. Molecular and cellular biology, [PMID:12370315] |
| 20. Vardar, Didem D, North, Christopher L CL, Sanchez-Irizarry, Cheryll C, Aster, Jon C JC and Blacklow, Stephen C SC.. (2003) Nuclear magnetic resonance structure of a prototype Lin12-Notch repeat module from human Notch1.. Biochemistry, (17): [PMID:12795601] |
| 21. Hambleton, Sophie S and 7 more authors.. (2004) Structural and functional properties of the human notch-1 ligand binding region.. Structure (London, England : 1993), [PMID:15576031] |
| 22. Ehebauer, Matthias T MT, Chirgadze, Dimitri Y DY, Hayward, Penny P, Martinez Arias, Alfonso A and Blundell, Tom L TL.. (2005) High-resolution crystal structure of the human Notch 1 ankyrin domain.. The Biochemical journal, (15): [PMID:16011479] |
| 23. Coleman, Mathew L ML and 12 more authors.. (2007) Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor.. The Journal of biological chemistry, (17): [PMID:17573339] |
| 24. Chastagner, Patricia P, Israël, Alain A and Brou, Christel C.. (2008) AIP4/Itch regulates Notch receptor degradation in the absence of ligand.. PloS one, (16): [PMID:18628966] |
| 25. Brütsch, René R and 8 more authors.. (2010) Integrin cytoplasmic domain-associated protein-1 attenuates sprouting angiogenesis.. Circulation research, (3): [PMID:20616313] |
| 26. Mo, Jung-Soon JS and 13 more authors.. (2011) Serum- and glucocorticoid-inducible kinase 1 (SGK1) controls Notch1 signaling by downregulation of protein stability through Fbw7 ubiquitin ligase.. Journal of cell science, (1): [PMID:21147854] |
| 27. Vasquez-Del Carpio, Rodrigo R and 6 more authors.. (2011) Assembly of a Notch transcriptional activation complex requires multimerization.. Molecular and cellular biology, [PMID:21245387] |
| 28. Gupta-Rossi, Neetu N and 6 more authors.. (2011) The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch pathway.. The Journal of biological chemistry, (27): [PMID:21464124] |
| 29. Lim, Seung-Oe SO and 7 more authors.. (2011) Notch1 binds and induces degradation of Snail in hepatocellular carcinoma.. BMC biology, (30): [PMID:22128911] |
| 30. Puca, Loredana L, Chastagner, Patricia P, Meas-Yedid, Vannary V, Israël, Alain A and Brou, Christel C.. (2013) Α-arrestin 1 (ARRDC1) and β-arrestins cooperate to mediate Notch degradation in mammals.. Journal of cell science, (1): [PMID:23886940] |
| 31. Boskovski, Marko T MT and 7 more authors.. (2013) The heterotaxy gene GALNT11 glycosylates Notch to orchestrate cilia type and laterality.. Nature, (19): [PMID:24226769] |
| 32. Chakrabarty, Broto and Parekh, Nita.. (2014) Identifying tandem Ankyrin repeats in protein structures.. BMC bioinformatics, (30): [PMID:25547411] |
| 33. Pinnell, Nancy N and 14 more authors.. (2015) The PIAS-like Coactivator Zmiz1 Is a Direct and Selective Cofactor of Notch1 in T Cell Development and Leukemia.. Immunity, (17): [PMID:26522984] |
| 34. Deatherage, Catherine L CL and 7 more authors.. (2017) Structural and biochemical differences between the Notch and the amyloid precursor protein transmembrane domains.. Science advances, [PMID:28439555] |
| 35. Takeuchi, Hideyuki H and 6 more authors.. (2018) Two novel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking and signaling.. Proceedings of the National Academy of Sciences of the United States of America, (4): [PMID:30127001] |
