Basic Description

Product Name	c-Fos Mouse mAb - Primary antibody, specific to c-Fos, Mouse IgG1
Synonyms	Cellular oncogene fos \| G0/G1 switch regulatory protein 7
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Host species	Mouse
Specificity	c-Fos
Immunogen	Purified recombinant human FOS protein fragments expressed in E.coli (AA 1-380).
Positive Control	WB: HCT116, COS7, HeLa(TSA treated ), HeLa lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Validated
Product Description	Mouse anti Human c-Fos Antibody, Monoclonal (7D6-A9-D4), could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

FOS Tbio Proto-oncogene c-Fos (0 Activities)

Discover Target: FOS

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0404677	Certificate of Analysis	Apr 19, 2024	Ab096159
ZJ24F0404676	Certificate of Analysis	Apr 19, 2024	Ab096159

FAQs and Articles

FAQ

Guidelines of Primary Antibodies for Western Blotting

Categories: Protocols

Tags:

Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

Protocol of Western Blotting For High Molecular Weights

Categories: Protocols

Tags:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

1. Ye N, Ding Y, Wild C, Shen Q, Zhou J.. (2014) Small molecule inhibitors targeting activator protein 1 (AP-1).. J Med Chem, 57 (16): (6930-6948). [PMID:24831826] [10.1021/jm5004733]
2. Roux, P P and 6 more authors.. (1991) Retrovirus-mediated gene transfer of a human c-fos cDNA into mouse bone marrow stromal cells.. Oncogene, [PMID:1658710]
3. Nakabeppu, Y Y and Nathans, D D.. (1989) The basic region of Fos mediates specific DNA binding.. The EMBO journal, (1): [PMID:2511004]
4. Hai, T W TW, Liu, F F, Coukos, W J WJ and Green, M R MR.. (1989) Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.. Genes & development, [PMID:2516827]
5. van Straaten, F F, Müller, R R, Curran, T T, Van Beveren, C C and Verma, I M IM.. (1983) Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6574479]
6. Glover, J N JN and Harrison, S C SC.. (1995) Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA.. Nature, (19): [PMID:7816143]
7. Zhang, Y Y, Feng, X H XH and Derynck, R R.. (1998) Smad3 and Smad4 cooperate with c-Jun/c-Fos to mediate TGF-beta-induced transcription.. Nature, (27): [PMID:9732876]
8. Heilig, Roland R and 98 more authors.. (2003) The DNA sequence and analysis of human chromosome 14.. Nature, (6): [PMID:12508121]
9. Bossis, Guillaume G and 9 more authors.. (2005) Down-regulation of c-Fos/c-Jun AP-1 dimer activity by sumoylation.. Molecular and cellular biology, [PMID:16055710]
10. Portal, M M MM, Ferrero, G O GO and Caputto, B L BL.. (2007) N-Terminal c-Fos tyrosine phosphorylation regulates c-Fos/ER association and c-Fos-dependent phospholipid synthesis activation.. Oncogene, (24): [PMID:17160021]
11. Jakobs, Astrid and 5 more authors.. (2007) Ubc9 fusion-directed SUMOylation identifies constitutive and inducible SUMOylation.. Nucleic acids research, [PMID:17709345]
12. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
13. Fiebich, Bernd L BL and 6 more authors.. (2012) Pseudoephedrine inhibits T-cell activation by targeting NF-κB, NFAT and AP-1 signaling pathways.. Immunopharmacology and immunotoxicology, [PMID:21631396]
14. Ferrero, G O GO, Velazquez, F N FN and Caputto, B L BL.. (2012) The kinase c-Src and the phosphatase TC45 coordinately regulate c-Fos tyrosine phosphorylation and c-Fos phospholipid synthesis activation capacity.. Oncogene, (12): [PMID:22105363]
15. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O.. (2015) SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage.. Cell reports, (17): [PMID:25772364]

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

SKU	Size	Availability	Price
Ab096159-10μl	10μl	In stock	$66.90
Ab096159-50μl	50μl	In stock	$189.90
Ab096159-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab096159-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,249.90

c-Fos Mouse mAb - Primary antibody, specific to c-Fos, Mouse IgG1