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c-Kit/CD117 Rat mAb - Primary antibody, specific to c-Kit/CD117, Rat IgG2b

Azide Free
Carrier Free
Validated
≥95%(SDS-PAGE)
Lot by Lot

Features and benefits

Short Overview:
mAb (ACK2); Rat anti Mouse c-Kit/CD117 Antibody; Flow, IP, in vivo mast cell depletion, in vivo c-Kit+ cell depletion, in vitro c-Kit neutralization; Unconjugated
Species reactivity(Reacts with): Mouse
Isotype: Rat IgG2b

Application:

Animal Model
Depletion
Flow Cytometry
Functional Assay
IP
Neutralization

Item Number

Ab096635

Grouped product items
SKU	Size	Availability	Price
Ab096635-10μg	10μg	In stock	$29.90
Ab096635-50μg	50μg	In stock	$69.90
Ab096635-100μg	100μg	In stock	$109.90
Ab096635-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$469.90

mAb (ACK2); Rat anti Mouse c-Kit/CD117 Antibody; Flow, IP, in vivo mast cell depletion, in vivo c-Kit+ cell depletion, in vitro c-Kit neutralization; Unconjugated

View related series

Accession#: P05532 c-Kit CD117 Gene ID: 16590

Basic Description

Product Name	c-Kit/CD117 Rat mAb - Primary antibody, specific to c-Kit/CD117, Rat IgG2b
Specifications & Purity	Validated, Azide Free, Carrier Free, ≥95%(SDS-PAGE), Lot by Lot
Host species	Rat
Specificity	c-Kit/CD117
Immunogen	IL-3-dependent mast cells
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, Validated
Product Description	Rat anti Mouse c-Kit/CD117 Antibody, Monoclonal (ACK2), could be used for Flow, IP, in vivo mast cell depletion, in vivo c-Kit+ cell depletion, in vitro c-Kit neutralization and so on. Application: Flow: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. in vivo mast cell depletion: Use at an assay dependent concentration. in vivo c-Kit+ cell depletion: Use at an assay dependent concentration. in vitro c-Kit neutralization: Use at an assay dependent concentration. Protein Function Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Product Properties

Isotype	Rat IgG2b
Light Chain Type	kappa
SDS-PAGE	26.0 kDa (Light Chain) & 52.5 kDa (Heavy Chain), under reducing conditions; 205.4 kDa, under non-reducing conditions.
Purification Method	Protein G purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at 2-8°C,Freeze prohibited
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon delivery aliquot.

Application

Application	Dilution info
IP	Use at an assay dependent concentration.
Flow	Use at an assay dependent concentration.
in vivo mast cell depletion	Use at an assay dependent concentration.
in vivo c-Kit+ cell depletion	Use at an assay dependent concentration.
in vitro c-Kit neutralization	Use at an assay dependent concentration.

Certificates

Certificate of Analysis(COA)

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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0708968	Certificate of Analysis	Jul 30, 2024	Ab096635
ZJ24F0708967	Certificate of Analysis	Jul 30, 2024	Ab096635
ZJ24F0708966	Certificate of Analysis	Jul 30, 2024	Ab096635

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c-Kit/CD117 Rat mAb - Primary antibody, specific to c-Kit/CD117, Rat IgG2b

Basic Description

Product Properties

Associated Targets

KIT Tclin Mast/stem cell growth factor receptor Kit 0 Activities