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c-Kit/CD117 Rat mAb - Primary antibody, specific to c-Kit/CD117, Rat IgG2b

Carrier Free

Validated

Azide Free

≥95%(SDS-PAGE)
See COA

Application:

Flow Cytometry
IF/ICC
IHC

Features and benefits

Host species: Rat
Species reactivity(Reacts with): Mouse
Isotype: Rat IgG2b
Clone Number: 2B8
Conjugation: Unconjugated

Item Number

Ab096644

Grouped product items
SKU	Size	Availability	Price
Ab096644-10μg	10μg	In stock	$29.90
Ab096644-50μg	50μg	In stock	$69.90
Ab096644-100μg	100μg	In stock	$109.90
Ab096644-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$449.90

mAb (2B8); Rat anti Mouse c-Kit/CD117 Antibody; Flow, ICC/IF, IHC; Unconjugated

View related series

Click to view target information: KIT

Accession#: P05532 c-Kit CD117 Gene ID: 16590

Basic Description

Product Name	c-Kit/CD117 Rat mAb - Primary antibody, specific to c-Kit/CD117, Rat IgG2b
Synonyms	W \| Bs \| Fdc \| Ssm \| SCO1 \| SCO5 \| SOW3 \| c-KIT \| Tr-kit \| Gsfsco1 \| Gsfsco5 \| Gsfsow3 \| CD117 antigen \| CD117 \| ckit \| c-kit \| EC 2.7.10 \| EC 2.7.10.1 \| KIT \| PBT \| piebald trait \| Proto-oncogene c-Kit \| proto-oncogene tyrosine-protein kinase Kit \| SCF R
Specifications & Purity	Carrier Free, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species	Rat
Specificity	c-Kit/CD117
Immunogen	Mouse bone marrow mast cells
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, Validated
Product Description	Rat anti Mouse c-Kit/CD117 Antibody, Monoclonal (2B8), could be used for Flow, ICC/IF, IHC and so on. Application: Flow: Use at an assay dependent concentration. ICC/IF: Use at an assay dependent concentration. IHC: Use at an assay dependent concentration. Protein Function Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Product Properties

Isotype	Rat IgG2b
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Form	Liquid
Concentration	See COA
Storage Temp	Store at 2-8°C,Freeze prohibited
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon delivery aliquot.

Associated Targets(Human)

KIT Tclin Mast/stem cell growth factor receptor Kit (0 Activities)

Discover Target: KIT

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

KIT Tclin Stem cell growth factor receptor (10667 Activities)

Discover Target: KIT

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

KIT Tclin VEGF-receptor 2 and stem cell growth factor receptor (KDR and KIT) (10 Activities)

Discover Target: KIT

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

KIT Tclin VHL/KIT (4 Activities)

Discover Target: KIT

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Application

Application	Dilution info
IHC	Use at an assay dependent concentration. "> Use at an assay dependent concentration.
IF/ICC	Use at an assay dependent concentration. "> Use at an assay dependent concentration.
Flow Cytometry	Use at an assay dependent concentration. "> Use at an assay dependent concentration.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0708971	Certificate of Analysis	Jul 30, 2024	Ab096644
ZJ24F0708970	Certificate of Analysis	Jul 30, 2024	Ab096644
ZJ24F0708969	Certificate of Analysis	Jul 30, 2024	Ab096644

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c-Kit/CD117 Rat mAb - Primary antibody, specific to c-Kit/CD117, Rat IgG2b

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Application

Certificates

Certificate of Analysis(COA)

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