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Calcein, AM - ≥96.0% (HPLC),suitable for fluorescence, high purity , CAS No.148504-34-1

38 Citations

for fluorescence analysis

≥96%(HPLC)

CAS#: 148504-34-1
Formula: C₄₆H₄₆N₂O₂₃
Molecular Weight: 994.87
EC Number: 200-664-3
MDL number: MFCD05861516
PubChem CID: 390986

Item Number

C131116

Grouped product items
SKU	Size	Availability	Price	Qty
C131116-1mg	1mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$432.90
C131116-5mg	5mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$1,510.90

Cell-permeable fluorescent dye for determining cell viability

Basic Description

Synonyms	Calcein AM \| Calcein-AM \| 148504-34-1 \| Glycine, N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, 1,1'-bis[(acetyloxy)methyl] ester \| acetyloxymethyl 2-[[2-(acetyloxy
Specifications & Purity	for fluorescence analysis, ≥96%(HPLC)
Storage Temp	Store at -20°C,Argon charged
Shipped In	Ice chest + Ice pads
Grade	for fluorescence analysis
Note	Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. For more information on AM esters please visit our AM esters FAQ page . Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description	Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein , which is an extremely fluorescent and negatively charged molecule. The probe can be used to stain living cells and has been observed to fluoresce bright green in cytoplasmic Schwann cells. Calcein-AM has been reported to be a neutral substrate for Mdr (P-glycoprotein (Pgp) and multidrug resistance protein (MRP)) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Studies suggest that Calcein-AM can be used to detect MDR mediated resistance and to be transported by PGP from the plasma membrane. Mitochondrial permeability research has utilized Calcein as a cytosolic fluorophore. Has been used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition. A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule

Associated Targets(Human)

A498 (42825 Activities)

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ACHN (49357 Activities)

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CAKI-1 (44928 Activities)

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CCRF-CEM (65223 Activities)

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COLO 205 (50209 Activities)

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DU-145 (51482 Activities)

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HL-60 (67320 Activities)

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HT-29 (80576 Activities)

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K562 (73714 Activities)

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M14 (47487 Activities)

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MCF7 (126967 Activities)

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MOLT-4 (49676 Activities)

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OVCAR-3 (48710 Activities)

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OVCAR-4 (44535 Activities)

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OVCAR-5 (45555 Activities)

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OVCAR-8 (47708 Activities)

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PC-3 (62116 Activities)

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RXF 393 (41971 Activities)

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SF-295 (48000 Activities)

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SK-MEL-2 (46422 Activities)

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SK-MEL-28 (48833 Activities)

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SK-MEL-5 (47095 Activities)

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SK-OV-3 (52876 Activities)

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SN12C (47755 Activities)

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SNB-19 (46794 Activities)

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TK-10 (45540 Activities)

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U-251 (51189 Activities)

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UACC-257 (46019 Activities)

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UACC-62 (47335 Activities)

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UO-31 (46270 Activities)

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786-0 (47912 Activities)

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A549 (127892 Activities)

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NCI/ADR-RES (33767 Activities)

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T47D (39041 Activities)

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EKVX (44102 Activities)

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NCI-H322M (45589 Activities)

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HCT-116 (91556 Activities)

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HOP-92 (41141 Activities)

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Hs-578T (29457 Activities)

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NCI-H460 (60772 Activities)

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SF-268 (49410 Activities)

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IGROV-1 (47897 Activities)

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LOX IMVI (44321 Activities)

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HOP-62 (47048 Activities)

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MDA-MB-435 (38290 Activities)

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MDA-N (28205 Activities)

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Malme-3M (44254 Activities)

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MDA-MB-231 (73002 Activities)

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SNB-75 (44215 Activities)

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NCI-H226 (44470 Activities)

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Names and Identifiers

IUPAC Name	acetyloxymethyl 2-[[2-(acetyloxymethoxy)-2-oxoethyl]-[[3',6'-diacetyloxy-7'-[[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]methyl]-3-oxospiro[2-benzofuran-1,9'-xanthene]-2'-yl]methyl]amino]acetate
INCHI	InChI=1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15-24H2,1-6H3
InChi Key	BQRGNLJZBFXNCZ-UHFFFAOYSA-N
Canonical SMILES	CC(=O)OCOC(=O)CN(CC1=CC2=C(C=C1OC(=O)C)OC3=C(C24C5=CC=CC=C5C(=O)O4)C=C(C(=C3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
Isomeric SMILES	CC(=O)OCOC(=O)CN(CC1=CC2=C(C=C1OC(=O)C)OC3=C(C24C5=CC=CC=C5C(=O)O4)C=C(C(=C3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
PubChem CID	390986
Molecular Weight	994.87

Certificates

Certificate of Analysis(COA)

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6 results found

Lot Number	Certificate Type	Date	Item
J2215198	Certificate of Analysis	Jul 18, 2024	C131116
F2208195	Certificate of Analysis	Mar 20, 2024	C131116
F2208205	Certificate of Analysis	Mar 20, 2024	C131116
I2116116	Certificate of Analysis	Jul 07, 2023	C131116
J2215196	Certificate of Analysis	Oct 20, 2022	C131116
I2321025	Certificate of Analysis	Aug 03, 2022	C131116

Chemical and Physical Properties

Sensitivity	light sensitive;Moisture sensitive

FAQs and Articles

FAQ

Fluorescent Probes - Absorption and Fluorescence

Categories: Technical articles

Tags:

Fluorescence Fuorescent Probe Fluorescent dyes

Citations of This Product

1. Zhang Q et al.. (2020) Adjustable and ultrafast light-cured hyaluronic acid hydrogel: promoting biocompatibility and cell growth.. J Mater Chem B, 8 (25): (5441-5450). [PMID:32555786]
2. Kai Yue, Chao Yang, Yu You, Xueying Wang, Xinxin Zhang. (2023) Experimental Investigation of Temperature Influence on Nanoparticle Adhesion in an Artificial Blood Vessel. International Journal of Nanomedicine, [PMID:36711003]
3. Xiaofang Du, Rong Zou, Kun Du, Dabing Huang, Chunguang Miao, Bensheng Qiu, Weiping Ding, Chengpan Li. (2023) Modeling Colorectal Cancer-Induced Liver Portal Vein Microthrombus on a Hepatic Lobule Chip. ACS Applied Materials & Interfaces, 15 (49): (56859–56868). [PMID:38033197]
4. Chunhong Sui, Yijia Luo, Xiao Xiao, Jiaxue Liu, Xiaotong Shao, Yingxue Xue, Cheng Wang, Wenliang Li. (2023) A Photothermal Therapy Study Based on Electrospinning Nanofibers Blended and Coated with Polydopamine Nanoparticles. ChemistrySelect, 8 (40): (e202302998). [PMID:16214896]
5. Meng-Wen Ma, Kai-Lin He, Chao-Qing Li, Xiao-Ting Xie, Sui Zhou, Bin Zhang, Xiao-Lin Hou, Cheng Li, Yuan-Di Zhao, Ling-Li Dong, Bo Liu. (2023) Multifunctional platinum-tellurium nanorods modified with hyaluronic acid remove reactive oxygen species to improve the photothermal treatment of rheumatoid arthritis. CHEMICAL ENGINEERING JOURNAL, 475 (13): (146450). [PMID:10644996]
6. Li Zhang, Qian Dong, Yumin Hao, Zihan Wang, Wenjuan Dong, Yang Liu, Yueping Dong, Hongpeng Wu, Shaomin Shuang, Chuan Dong, Zhuo Chen, Xiaojuan Gong. (2023) Drug-Primed Self-Assembly of Platinum-Single-Atom Nanozyme to Regulate Cellular Redox Homeostasis Against Cancer. Advanced Science, (5): (2302703). [PMID:37697645]
7. Pengyu Zang, Yaqian Du, Chenghao Yu, Dan Yang, Shili Gai, Lili Feng, Shikai Liu, Piaoping Yang, Jun Lin. (2023) Photothermal-Actuated Thermoelectric Therapy by Harnessing Janus-Structured Ag–Ag2S Nanoparticles with Enhanced Antitumor Efficacy. CHEMISTRY OF MATERIALS, (23): [PMID:19011241]
8. Minglu Tang, Zhengya Yue, Jialun Li, Tiedong Sun, Chunxia Chen. (2023) Nanozymes Based on MXene Nanosheets Decorated with Pt Nanoparticles for Hyperthermal-Enhanced Cascade Catalytic Therapy of Tumors. ACS Applied Nano Materials, 6 (15): (14609–14616). [PMID:]
9. Li Shibo, Li Chengpan, Khan Muhammad Imran, Liu Jing, Shi Zhengdi, Gao Dayong, Qiu Bensheng, Ding Weiping. (2023) Microneedle array facilitates hepatic sinusoid construction in a large-scale liver-acinus-chip microsystem. Microsystems & Nanoengineering, 9 (1): (1-14). [PMID:37303831]
10. Jun Cao, Tao Zhang, Wei Zhu, Houbin Li, Aiguo Shen. (2023) A cooling-driven self-adaptive and removable hydrogel coupled with combined chemo-photothermal sterilization for promoting infected wound healing. Nanoscale, [PMID:37340945]
11. Jie Cheng, Jinxin Yan, Ying Liu, Jiangzhou Shi, Haoyu Wang, Hanyang Zhou, Yinglin Zhou, Tongcun Zhang, Lina Zhao, Xianbin Meng, Haipeng Gong, Xinxiang Zhang, Haichuan Zhu, Peng Jiang. (2023) Cancer-cell-derived fumarate suppresses the anti-tumor capacity of CD8+ T cells in the tumor microenvironment. Cell Metabolism, 35 (961-978.e10). [PMID:37178684]
12. Ruoxi Zhao, Haixia Zhu, Lili Feng, Yanlin Zhu, Bin Liu, Chenghao Yu, Shili Gai, Piaoping Yang. (2023) 2D Piezoelectric BiVO4 Artificial Nanozyme with Adjustable Vanadium Vacancy for Ultrasound Enhanced Piezoelectric/Sonodynamic Therapy. Small, (2301349). [PMID:37127877]
13. Zhilin Dai, Chengpan Li, Zhengdi Shi, Shibo Li, Tianzhi Luo, Weiping Ding. (2023) A glomerulus chip with spherically twisted cell-laden hollow fibers as glomerular capillary tufts. Biofabrication, 15 (3): (35004). [PMID:36898152]
14. Jingjing Zhou, Ruitao Cha, Zhiyu Wu, Chunliang Zhang, Yuhang He, Hanru Zhang, Kexin Liu, Muhammad Subaan Fareed, Zhaopeng Wang, Changyan Yang, Yifan Zhang, Wenjin Yan, Kairong Wang. (2023) An injectable, natural peptide hydrogel with potent antimicrobial activity and excellent wound healing-promoting effects. Nano Today, 49 (101801). [PMID:]
15. Zhu Yuntian, Shi Zhengdi, Ding Weiping, Li Chengpan. (2023) On-chip construction of a fully structured scaffold-free vascularized renal tubule. BIOMEDICAL MICRODEVICES, 25 (1): (1-13). [PMID:36826720]
16. Na An, Yan Gao, Ying Shi, Xingxing Du, Jianing Cheng, Shuanglong Tang, Pengfei Liu, Huike Yang, Chongshen Guo. (2023) Nickel phosphide for MRI-guided synergistic photo-immunotherapy. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY, 151 (150). [PMID:]
17. Zhifang Li, Shulong Wang, Jingjin Zhao, Yanni Luo, Hong Liang, Shulin Zhao, Liangliang Zhang. (2023) Gold Nanocluster Encapsulated Nanorod for Tumor Microenvironment Simultaneously Activated NIR-II Photoacoustic/Photothermal Imaging and Cancer Therapy. Advanced Therapeutics, 6 (4): (2200350). [PMID:]
18. Tu Jun, Zeng Yuyang, An Ran, Sun Jiaming, Wen Huicai. (2023) Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors. Scientific Reports, 13 (1): (1-13). [PMID:36639385]
19. Lu Yang, Zhao Wang, HaiJiang Gong, Shili Gai, RuiFang Shen. (2023) Tirapazamine-loaded UiO-66/Cu for ultrasound-mediated promotion of chemodynamic therapy cascade hypoxia-activated anticancer therapy. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 634 (495). [PMID:36542978]
20. Chao-Qing Li, Xiao-Lin Hou, Dong-Xue Jiang, Bin Zhang, Meng-Wen Ma, Xiao-Ting Xie, Yuan-Di Zhao, Tian-Cai Liu, Bo Liu. (2022) Binary Pt/Te Nanoheterostructures with High Photothermal Conversion Efficiency and Anti-inflammatory Action for Enhanced Photothermal Therapy of 4T1 Breast Tumors Guided by Photoacoustic Imaging. ACS Sustainable Chemistry & Engineering, 10 (50): (16598–16610). [PMID:]
21. Li Li, Xulin Xu, Keyu Lv, Guijuan Zheng, Hao Wang, Shuai Chen, Lang Huang, Yi Liu, Yadong Zhang, Zhaoming Tang, Lili Zhang, Jinyu Wang, Jianlin Qiao, Hongliang Li, Xuanbin Wang, Guangmin Yao, Chao Fang. (2022) Asebogenin suppresses thrombus formation via inhibition of Syk phosphorylation. BRITISH JOURNAL OF PHARMACOLOGY, 180 (3): (287-307). [PMID:36166754]
22. Xiaoxiao He, Xiaohong Ma, Yujun Yang, Xi Hu, Teng Wang, Shiyue Chen, Xiang Mao. (2022) Metal Cluster Triggered-Assembling Heterogeneous Au-Ag Nanoclusters with Highly Loading Performance and Biocompatible Capability. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (19): (11197). [PMID:36232494]
23. Xiaohui Yang, Xixia Liu, Fangshen Xu, Shunxian Ji, Yuchao Sun, Zhaojun Song, Jie Song, Yulian Wu, Jun Yin. (2022) Fabrication of microgroove poly(lactic-co-glycolic acid) nerve guide conduit using dry-jet wet spinning for rat laryngeal recurrent nerve regeneration. MATERIALS & DESIGN, 223 (111151). [PMID:]
24. Sijin Liu, Xi Zhao, Sufang Shui, Biao Wang, Yingxian Cui, Suwei Dong, Tairan Yuwen, Guoquan Liu. (2022) PDTAC: Targeted Photodegradation of GPX4 Triggers Ferroptosis and Potent Antitumor Immunity. JOURNAL OF MEDICINAL CHEMISTRY, 65 (18): (12176–12187). [PMID:36066386]
25. Lai Zhao, Xinquan Gu, Fuquan Jiang, Bo Li, Shuang Lu, Fan Wang, Yao Sun, Kai Liu, Jingjing Li. (2022) Long-Lasting Proteinaceous Nanoformulation for Tumor Imaging and Therapy. ACS Omega, 7 (35): (31299–31308). [PMID:36092568]
26. Jingjing Zhou, Zhaopeng Wang, Changyan Yang, Hanru Zhang, Muhammad Subaan Fareed, Yuhang He, Jie Su, Panpan Wang, Zhiqiang Shen, Wenjin Yan, Kairong Wang. (2022) A carrier-free, dual-functional hydrogel constructed of antimicrobial peptide Jelleine-1 and 8Br-cAMP for MRSA infected diabetic wound healing. Acta Biomaterialia, 151 (223). [PMID:35948174]
27. Jiadai Zhai, Shucheng Li, Lin Fu, Chuang Li, Bingxia Sun, Feng Sang, Hongliang Liu. (2022) Structural modification and antibacterial property studies of natural chalcone sanjuanolide.. Frontiers in Chemistry, 10 (959250-959250). [PMID:35991609]
28. Minglu Tang, Zhaocong Zhang, Chengwen Ding, Jingqi Li, Yangtian Shi, Tiedong Sun, Chunxia Chen. (2022) Two birds with one stone: innovative ceria-loaded gold@platinum nanospheres for photothermal-catalytic therapy of tumors. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 627 (299). [PMID:35863189]
29. Rui Zhang, Changlin Liu, Ruoxi Zhao, Yaqian Du, Dan Yang, He Ding, Guixin Yang, Shili Gai, Fei He, Piaoping Yang. (2022) Engineering oxygen vacancy of MoOx nanoenzyme by Mn doping for dual-route cascaded catalysis mediated high tumor eradication. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 623 (155). [PMID:35576647]
30. Zhen Zhao, Hua Zhang, Hongda Chen, Yuanhong Xu, Lina Ma, Zhenxin Wang. (2022) An efficient photothermal–chemotherapy platform based on a polyacrylamide/phytic acid/polydopamine hydrogel. Journal of Materials Chemistry B, 10 (21): (4012-4019). [PMID:35506396]
31. Hongliang You, Dao Wang, Linlin Wei, Jiao Chen, Huanhuan Li, Yufeng Liu. (2022) Deferoxamine Inhibits Acute Lymphoblastic Leukemia Progression through Repression of ROS/HIF-1α, Wnt/β-Catenin, and p38MAPK/ERK Pathways. Journal of Oncology, 2022 (8281267). [PMID:35237325]
32. Liu Yu, Shang Wenting, Liu Heng, Hui Hui, Wu Jun, Zhang Wei, Gao Pengli, Guo Kunxiong, Guo Yanli, Tian Jie. (2022) Biomimetic manganese-eumelanin nanocomposites for combined hyperthermia-immunotherapy against prostate cancer. JOURNAL OF NANOBIOTECHNOLOGY, 20 (1): (1-15). [PMID:35073918]
33. Zhe Yang, Li Zhang, Jielin Wei, Ruiqi Li, Qi Xu, Han Hu, Zushun Xu, Jinghua Ren, Chun-Yuen Wong. (2022) Tumor acidity-activatable photothermal/Fenton nanoagent for synergistic therapy. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 612 (355). [PMID:34998195]
34. Xiaoning Ren, Wenliang Liu, Houjian Zhou, Jianshuang Wei, Congpu Mu, Ying Wan, Xiaoquan Yang, Anmin Nie, Zhongyuan Liu, Xiangliang Yang, Zhiqiang Luo. (2022) Biodegradable 2D GeP nanosheets with high photothermal conversion efficiency for multimodal cancer theranostics. CHEMICAL ENGINEERING JOURNAL, 431 (134176). [PMID:]
35. Chao-Qing Li, Meng-Wen Ma, Bin Zhang, Wei Chen, Zhong-Yuan Yin, Xiao-Ting Xie, Xiao-Lin Hou, Yuan-Di Zhao, Bo Liu. (2022) A self-assembled nanoplatform based on Ag2S quantum dots and tellurium nanorods for combined chemo-photothermal therapy guided by H2O2-activated near-infrared-II fluorescence imaging. Acta Biomaterialia, 140 (547). [PMID:34923095]
36. Deng Xingyu, Chen Xiabin, Geng Fang, Tang Xin, Li Zhenzhen, Zhang Jie, Wang Yikai, Wang Fangqian, Zheng Na, Wang Peng, Yu Xiaohua, Hou Shurong, Zhang Wei. (2021) Precision 3D printed meniscus scaffolds to facilitate hMSCs proliferation and chondrogenic differentiation for tissue regeneration. JOURNAL OF NANOBIOTECHNOLOGY, 19 (1): (1-19). [PMID:34856996]
37. Shanshan Li, Kunpeng Lin, Peng Hu, Shaochen Wang, Shuang Zhao, Ying Gan, Lei Liu, Shuling Yu, Jiahua Shi. (2022) A multifunctional nanoamplifier with self-enhanced acidity and hypoxia relief for combined photothermal/photodynamic/starvation therapy. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 611 (121307). [PMID:34798156]
38. Song Chai,Shifeng Kan,Ran Sun,Ruijuan Zhou,Yi Sun,Wenhua Chen,Bo Yu. (2018-12-05) Fabricating polydopamine-coated MoSe2-wrapped hollow mesoporous silica nanoplatform for controlled drug release and chemo-photothermal therapy.. International journal of nanomedicine, 13 (7607-7621). [PMID:30510420]

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5. Derici GE et al.. (2021) Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes.. PLoS One, 16 (6): (e0252707). [PMID:34133443]
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