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Calcein, AM - ≥96.0% (HPLC),suitable for fluorescence, high purity , CAS No.148504-34-1

70 Citations
Item Number
C131116
Grouped product items
SKUSizeAvailabilityPrice Qty
C131116-1mg
1mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$432.90
C131116-5mg
5mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$1,510.90
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Cell-permeable fluorescent dye for determining cell viability

Basic Description

SynonymsAC1L916S | acetoxymethyl 2-[[2-(acetoxymethoxy)-2-oxo-ethyl]-[[3',6'-diacetoxy-7'-[[bis[2-(acetoxymethoxy)-2-oxo-ethyl]amino]methyl]-3-oxo-spiro[isobenzofuran-1,9'-xanthene]-2'-yl]methyl]amino]acetate | Tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diace
Specifications & Purityfor fluorescence analysis, ≥96%(HPLC)
Storage TempStore at -20°C,Argon charged
Shipped InIce chest + Ice pads
Gradefor fluorescence analysis
NoteWherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. For more information on AM esters please visit our AM esters FAQ page . Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description

Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein , which is an extremely fluorescent and negatively charged molecule. The probe can be used to stain living cells and has been observed to fluoresce bright green in cytoplasmic Schwann cells. Calcein-AM has been reported to be a neutral substrate for Mdr (P-glycoprotein (Pgp) and multidrug resistance protein (MRP)) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Studies suggest that Calcein-AM can be used to detect MDR mediated resistance and to be transported by PGP from the plasma membrane. Mitochondrial permeability research has utilized Calcein as a cytosolic fluorophore. Has been used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition.
A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule

Associated Targets(Human)

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ACHN (49357 Activities)
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IGROV-1 (47897 Activities)
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LOX IMVI (44321 Activities)
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HOP-62 (47048 Activities)
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MDA-MB-435 (38290 Activities)
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MDA-N (28205 Activities)
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Malme-3M (44254 Activities)
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SNB-75 (44215 Activities)
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NCI-H226 (44470 Activities)
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Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Names and Identifiers

IUPAC Name acetyloxymethyl 2-[[2-(acetyloxymethoxy)-2-oxoethyl]-[[3',6'-diacetyloxy-7'-[[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]methyl]-3-oxospiro[2-benzofuran-1,9'-xanthene]-2'-yl]methyl]amino]acetate
INCHI InChI=1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15-24H2,1-6H3
InChi Key BQRGNLJZBFXNCZ-UHFFFAOYSA-N
Canonical SMILES CC(=O)OCOC(=O)CN(CC1=CC2=C(C=C1OC(=O)C)OC3=C(C24C5=CC=CC=C5C(=O)O4)C=C(C(=C3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
Isomeric SMILES CC(=O)OCOC(=O)CN(CC1=CC2=C(C=C1OC(=O)C)OC3=C(C24C5=CC=CC=C5C(=O)O4)C=C(C(=C3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
PubChem CID 390986
Molecular Weight 994.87

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

6 results found

Lot NumberCertificate TypeDateItem
J2215198Certificate of AnalysisJul 18, 2024 C131116
F2208195Certificate of AnalysisMar 20, 2024 C131116
F2208205Certificate of AnalysisMar 20, 2024 C131116
I2116116Certificate of AnalysisJul 07, 2023 C131116
J2215196Certificate of AnalysisOct 20, 2022 C131116
I2321025Certificate of AnalysisAug 03, 2022 C131116

Chemical and Physical Properties

Sensitivitylight sensitive;Moisture sensitive

Related Documents

 SDS

 Specification Sheet

FAQs and Articles

FAQ
Fluorescent Probes - Absorption and Fluorescence
Categories: Technical articles
Tags:
Fluorescence Fuorescent Probe Fluorescent dyes

Citations of This Product

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