Calponin 1 Antibody - Primary antibody, specific to Calponin 1, Rabbit IgG

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Dog,Human,Mouse,Pig,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab092561
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab092561-10μl
10μl
In stock
$69.90
Ab092561-50μl
50μl
In stock
$209.90
Ab092561-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$329.90
Ab092561-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

pAb; Rabbit anti Human Calponin 1 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameCalponin 1 Antibody - Primary antibody, specific to Calponin 1, Rabbit IgG
SynonymsBasic calponin antibody | Calponin 1 antibody | Calponin 1 basic smooth muscle antibody | Calponin H1 antibody | Calponin H1 smooth muscle antibody | Calponin-1 antibody | Calponin1 antibody | Calponins basic antibody | CNN 1 antibody | Cnn1 antibody | CN
Specifications & PurityExactAb™, Validated, 0.4 mg/mL
Host speciesRabbit
SpecificityCalponin 1
ImmunogenA synthetic peptide derived from human Calponin (AA 250-297)
Positive ControlWB: Mouse heart, K562, HeLa, NIH/3T3 and C6 lysates. IHC: Human breast tissue.
ConjugationUnconjugated
GradeExactAb™, Validated
Product Description

Rabbit anti Human Calponin 1 Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

  • WB: 1/500-1/1000
  • IHC: 1/50-1/100

Protein Function

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.4 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Calponin 1 Antibody (Ab092561) - Western Blot
All lanes: Calponin 1 Antibody (Ab092561) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 32, 34 kDa
Exposure time: 22.5 s

Calponin 1 Antibody (Ab092561) - Western Blot
All lanes: Calponin 1 Antibody (Ab092561) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 32, 34 kDa
Exposure time: 20.0 s

Calponin 1 Antibody (Ab092561) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast using Calponin 1 Antibody (Ab092561). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

CNN1 Tbio Calponin-1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IHC1/50 - 1/100

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1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0202830Certificate of AnalysisFeb 23, 2024 Ab092561
ZJ24F0202831Certificate of AnalysisFeb 23, 2024 Ab092561

Related Documents

References

1. Maguchi, M M and 5 more authors..  (1995)  Molecular cloning and gene mapping of human basic and acidic calponins..  Biochemical and biophysical research communications,    (5):   [PMID:8526917]
2. Miano, J M JM, Krahe, R R, Garcia, E E, Elliott, J M JM and Olson, E N EN..  (1997)  Expression, genomic structure and high resolution mapping to 19p13.2 of the human smooth muscle cell calponin gene..  Gene,    (15):   [PMID:9332369]
3. Grimwood, Jane J and 97 more authors..  (2004)  The DNA sequence and biology of human chromosome 19..  Nature,    (1):   [PMID:15057824]

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