A synthetic peptide derived from human Calponin (AA 250-297)
Positive Control
WB: Mouse heart, K562, HeLa, NIH/3T3 and C6 lysates. IHC: Human breast tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Product Description
Rabbit anti Human Calponin 1 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.4 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Calponin 1 Antibody (Ab092561) - Western Blot All lanes: Calponin 1 Antibody (Ab092561) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa Observed band size: 32, 34 kDa Exposure time: 22.5 s
Calponin 1 Antibody (Ab092561) - Western Blot All lanes: Calponin 1 Antibody (Ab092561) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa Observed band size: 32, 34 kDa Exposure time: 20.0 s
Calponin 1 Antibody (Ab092561) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast using Calponin 1 Antibody (Ab092561). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
1.Maguchi, M M and 5 more authors.. (1995) Molecular cloning and gene mapping of human basic and acidic calponins.. Biochemical and biophysical research communications, (5):[PMID:8526917]
2.Miano, J M JM, Krahe, R R, Garcia, E E, Elliott, J M JM and Olson, E N EN.. (1997) Expression, genomic structure and high resolution mapping to 19p13.2 of the human smooth muscle cell calponin gene.. Gene, (15):[PMID:9332369]
3.Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1):[PMID:15057824]
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