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Caspase-7 Mouse mAb - Primary antibody, specific to Caspase-7, IgG1

    Application:
  • ELISA
  • Flow Cytometry
  • IHC
  • WB
Features and benefitsView More
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
  • Clone Number: 4D10B2
  • Conjugation: Unconjugated
Item Number
Ab093049
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab093049-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$129.90
Ab093049-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$387.90
Ab093049-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$597.90
Ab093049-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,937.90

mAb (4D10B2); Mouse anti Human Caspase-7 Antibody; WB, IHC, Flow, ELISA; Unconjugated

Basic Description

Product NameCaspase-7 Mouse mAb - Primary antibody, specific to Caspase-7, IgG1
SynonymsApoptotic protease Mch-3 antibody | Apoptotic protease MCH3 antibody | CASP-7 antibody | CASP7 antibody | CASP7_HUMAN antibody | Caspase 7 antibody | Caspase 7 apoptosis related cysteine peptidase antibody | Caspase-7 subunit p11 antibody | Caspase7 antib
Specifications & PurityExactAb™, Validated, Carrier Free, 1.0mg/mL
Host speciesMouse
SpecificityCaspase-7
ImmunogenPurified recombinant fragment of human CASP-7 (AA: 29-198) expressed in E. Coli.
Positive ControlWB: Human Caspase-7 (aa29-198) recombinant protein; Caspase-7 (aa29-198)-hIgGFc transfected HEK293 cell lysate; Jurkat, HEK293, MOLT4, MCF 7 and PC-12 cell lysates; Flow: MCF7 cells; IHC: Human cervical cancer tissue
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Mouse monoclonal (4D10B2) to Caspase-7, could be used for WB, IHC, Flow and ELISA.
Application:
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000

Protein Function:
CASP7 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product Properties

IsotypeIgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration1.0mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStorage: 4℃; -20℃ for long term storage. Avoid repeated freeze/thaw cycles.

Images

Caspase-7 Mouse mAb (Ab093049) - Western Blot
All lanes: Caspase-7 Mouse mAb (Ab093049) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 34 kDa
Observed band size: 33 kDa

Caspase-7 Mouse mAb (Ab093049) - Flow Cytometry
Flow cytometric analysis of MCF7 cells labeling Caspase-7 Mouse mAb (Ab093049) at 1/200 dilution (green) compared to a negative control (red).

Caspase-7 Mouse mAb (Ab093049) - IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Caspase-7 with Caspase-7 Mouse mAb (Ab093049) at 1/200 dilution.

Associated Targets(Human)

CASP7 Tchem Caspase-7 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/2000

">

1/500-1/2000

Flow Cytometry

1/200-1/400

ELISA

1/10000

IHC

1/200-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Enter lot number to search for analytical chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0700745Certificate of AnalysisAug 01, 2023 Ab093049
ZJ23F0700744Certificate of AnalysisAug 01, 2023 Ab093049

Related Documents

FAQs and Articles

FAQ
Flow Cytometry
Flow cytometry analysis
Flow cytometry protocol
Flow cytometry cell cycle analysis using propidium iodide DNA staining
Experimental Guide of Sample Preparation for Flow Cytometry
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Immunohistochemistry (IHC) staining antigen retrieval protocol
Caspase Immunofluorescence Staining Protocol
Apoptosis Induction Methods

References

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