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Caspase-9 Mouse mAb - Primary antibody, specific to CASP9, Mouse IgG1

    Application:
  • WB
Features and benefitsView More
  • Host species: Mouse
  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: Mouse IgG1
  • Clone Number: 1D1-F2-E9
  • Conjugation: Unconjugated
Item Number
Ab093085
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab093085-10μl
10μl
In stock
$66.90
Ab093085-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$199.90
Ab093085-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$295.90
Ab093085-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

mAb(1D1-F2-E9); Mouse anti Human Caspase-9 Antibody; WB; Unconjugated

Basic Description

Product NameCaspase-9 Mouse mAb - Primary antibody, specific to CASP9, Mouse IgG1
SynonymsCASP9 | MCH6 | Caspase-9 | CASP-9 | Apoptotic protease Mch-6 | Apoptotic protease-activating factor 3 | APAF-3 | ICE-like apoptotic protease 6 | ICE-LAP6 | APAF-3 antibody | APAF3 antibody | Apoptosis related cysteine peptidase antibody | Apoptotic protea
Specifications & PurityExactAb™, Validated, 2.05 mg/mL
Host speciesMouse
SpecificityCASP9
ImmunogenPurified recombinant human Caspase-9 protein fragments expressed in E.coli (AA 1-416).
Positive ControlWB: A549, A431, Jurkat, K562, NIH/3T3 and HeLa cell lysates.
ConjugationUnconjugated
GradeExactAb™, Validated
Product Description

Mouse anti Human Caspase-9 Antibody, Monoclonal(1D1-F2-E9), could be used for WB and so on .
Application:
WB: 1/500-1/1000
Protein Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Product Properties

IsotypeMouse IgG1
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration2.05 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Caspase-9 Mouse mAb (Ab093085) - Western Blot
All lanes: Caspase-9 Mouse mAb (Ab093085) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution

Predicted band size: 46 kDa
Observed band size: 27, 43, 45 kDa
Exposure time: 30 s

Associated Targets(Human)

CASP9 Tchem Caspase-9 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
CASP9 Tchem Caspase-9 (154 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences
Caspase-9 inhibitorINHIBITORALA2273Caspase-9SINGLE PROTEINHomo sapiens

PubMed: [1]


Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Enter lot number to search for analytical chart:

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404300Certificate of AnalysisApr 09, 2024 Ab093085
ZJ23F0901048Certificate of AnalysisSep 14, 2023 Ab093085
ZJ23F0901049Certificate of AnalysisSep 14, 2023 Ab093085

Related Documents

References

1. Mocanu MM, Baxter GF, Yellon DM.  (2000)  Caspase inhibition and limitation of myocardial infarct size: protection against lethal reperfusion injury..  Br J Pharmacol,  130  (2): (197-200).  [PMID:10807653] [10.1021/op500134e]
2. Zhou G, Zorn N, Ting P, Aslanian R, Lin M, Cook J, Lachowicz J, Lin A, Smith M, Hwa J et al..  (2014)  Development of novel benzomorpholine class of diacylglycerol acyltransferase I inhibitors..  ACS Med Chem Lett,  (5): (544-9).  [PMID:24900877] [10.1021/op500134e]
3. Kwan JC, Eksioglu EA, Liu C, Paul VJ, Luesch H.  (2009)  Grassystatins A-C from marine cyanobacteria, potent cathepsin E inhibitors that reduce antigen presentation..  J Med Chem,  52  (18): (5732-47).  [PMID:19715320] [10.1021/op500134e]
4. Salvador LA, Taori K, Biggs JS, Jakoncic J, Ostrov DA, Paul VJ, Luesch H..  (2013)  Potent elastase inhibitors from cyanobacteria: structural basis and mechanisms mediating cytoprotective and anti-inflammatory effects in bronchial epithelial cells..  J Med Chem,  56  (3): (1276-1290).  [PMID:23350733] [10.1021/jm3017305]
5. Duan, H H and 6 more authors..  (1996)  ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B..  The Journal of biological chemistry,    (12):   [PMID:8663294]
6. Srinivasula, S M SM and 9 more authors..  (1996)  The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32..  The Journal of biological chemistry,    (25):   [PMID:8900201]
7. Seol, D W DW and Billiar, T R TR..  (1999)  A caspase-9 variant missing the catalytic site is an endogenous inhibitor of apoptosis..  The Journal of biological chemistry,    (22):   [PMID:9890966]
8. Srinivasula, S M SM and 6 more authors..  (1999)  Identification of an endogenous dominant-negative short isoform of caspase-9 that can regulate apoptosis..  Cancer research,    (1):   [PMID:10070954]
9. Hadano, S S and 9 more authors..  (1999)  Genomic organization of the human caspase-9 gene on Chromosome 1p36. 1-p36.3..  Mammalian genome : official journal of the International Mammalian Genome Society,      [PMID:10384055]
10. Renatus, M M, Stennicke, H R HR, Scott, F L FL, Liddington, R C RC and Salvesen, G S GS..  (2001)  Dimer formation drives the activation of the cell death protease caspase 9..  Proceedings of the National Academy of Sciences of the United States of America,    (4):   [PMID:11734640]
11. Riebeling, C C, Forsea, A-M AM, Raisova, M M, Orfanos, C E CE and Geilen, C C CC..  (2002)  The bisphosphonate pamidronate induces apoptosis in human melanoma cells in vitro..  British journal of cancer,    (29):   [PMID:12177810]
12. Shiozaki, Eric N EN and 8 more authors..  (2003)  Mechanism of XIAP-mediated inhibition of caspase-9..  Molecular cell,      [PMID:12620238]
13. Allan, Lindsey A LA and 5 more authors..  (2003)  Inhibition of caspase-9 through phosphorylation at Thr 125 by ERK MAPK..  Nature cell biology,      [PMID:12792650]
14. Bartke, Till T, Pohl, Christian C, Pyrowolakis, George G and Jentsch, Stefan S..  (2004)  Dual role of BRUCE as an antiapoptotic IAP and a chimeric E2/E3 ubiquitin ligase..  Molecular cell,    (18):   [PMID:15200957]
15. Raina, Deepak D and 7 more authors..  (2005)  c-Abl tyrosine kinase regulates caspase-9 autocleavage in the apoptotic response to DNA damage..  The Journal of biological chemistry,    (25):   [PMID:15657060]
16. Wada, Akira A and 7 more authors..  (2006)  Pamidronate induced anti-proliferative, apoptotic, and anti-migratory effects in hepatocellular carcinoma..  Journal of hepatology,      [PMID:16298452]
17. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,    (18):   [PMID:16710414]
18. Wang, Pingzhang P, Shi, Taiping T and Ma, Dalong D..  (2006)  Cloning of a novel human caspase-9 splice variant containing only the CARD domain..  Life sciences,    (1):   [PMID:16780893]
19. Han, Yuchi Y and 7 more authors..  (2006)  Overexpression of HAX-1 protects cardiac myocytes from apoptosis through caspase-9 inhibition..  Circulation research,    (18):   [PMID:16857965]
20. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,    (5):   [PMID:18669648]
21. Kim, Jae-Hong JH and 8 more authors..  (2009)  HIP1R interacts with a member of Bcl-2 family, BCL2L10, and induces BAK-dependent cell death..  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology,      [PMID:19255499]
22. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,    (4):   [PMID:23186163]
23. Blasche, Sonja and 12 more authors..  (2013)  The E. coli effector protein NleF is a caspase inhibitor..  PloS one,      [PMID:23516580]
24. Eron, Scott J SJ, Raghupathi, Kishore K and Hardy, Jeanne A JA..  (2017)  Dual Site Phosphorylation of Caspase-7 by PAK2 Blocks Apoptotic Activity by Two Distinct Mechanisms..  Structure (London, England : 1993),    (3):   [PMID:27889207]
25. Smith, Christina E and 5 more authors..  (2017)  Non-steroidal Anti-inflammatory Drugs Are Caspase Inhibitors..  Cell chemical biology,    (16):   [PMID:28238723]

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