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CCK4 Mouse mAb

    Application:
  • IP
  • WB
Features and benefitsView More
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
  • Clone Number: K40054_12F7
  • Conjugation: Unconjugated
Item Number
Ab093426
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab093426-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab093426-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab093426-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab093426-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

mAb (K40054_12F7); Mouse anti Human CCK4 Antibody; WB, IP; Unconjugated

Basic Description

Product NameCCK4 Mouse mAb
SynonymsTyrosine-protein kinase-like 7 | PTK7 | PTK7 protein tyrosine kinase 7 | Pseudo tyrosine kinase receptor 7 | CCK 4 antibody | 430404F20Rik antibody | CCK-4 antibody | Colon carcinoma kinase 4 antibody | Human colon carcinoma kinase 4 (CCK4) mRNA complete
Specifications & PurityExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host speciesMouse
SpecificityCCK4
ImmunogenRecombinant protein of human CCK4 (AA 41-210).
Positive ControlWB: Hep G2, HeLa, Jurkat, A431 and HEK293 cell lysates. IP: HCT116 lysates.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Mouse anti Human CCK4 Antibody, Monoclonal (K40054_12F7), could be used for WB, IP and so on.
Application:
WB: 1/1000
IP: 1/100
Protein Function:
Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -80°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And Storage Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

CCK4 Mouse mAb (Ab093426) - Western Blot
All lanes: CCK4 Mouse mAb (Ab093426) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 118 kDa
Observed band size: 70, 155 kDa
Exposure time: 104.7 s

CCK4 Mouse mAb (Ab093426) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of CCK4 Mouse mAb (Ab093426) with HCT116 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. CCK4 Mouse mAb (Ab093426) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: HCT116 lysateLane 2: PTK7 immunoprecipitated from HCT116 lysate by CCK4 Mouse mAb (Ab093426)
Lane 3: The isotype control antibody instead of CCK4 Mouse mAb (Ab093426) in HCT116 lysate. Result: CCK4 Mouse mAb (Ab093426) can immunoprecipitate PTK7.

Associated Targets(Human)

PTK7 Tbio Inactive tyrosine-protein kinase 7 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB 1/1000

">

1/1000

IP

1/100

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Enter lot number to search for analytical chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0708923Certificate of AnalysisJul 29, 2024 Ab093426
ZJ24F0708922Certificate of AnalysisJul 29, 2024 Ab093426

Related Documents

Solution Calculators