WB: Hep G2, HeLa, Jurkat, A431 and HEK293 cell lysates. IP: HCT116 lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Product Description
Mouse anti Human CCK4 Antibody, Monoclonal (K40054_12F7), could be used for WB, IP and so on. Application: WB: 1/1000 IP: 1/100 Protein Function: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
Product Properties
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -80°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
CCK4 Mouse mAb (Ab093426) - Western Blot All lanes: CCK4 Mouse mAb (Ab093426) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 118 kDa Observed band size: 70, 155 kDa Exposure time: 104.7 s
CCK4 Mouse mAb (Ab093426) - IP Immunoprecipitation was performed by incubation of 2.5 µg of CCK4 Mouse mAb (Ab093426) with HCT116 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. CCK4 Mouse mAb (Ab093426) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: HCT116 lysateLane 2: PTK7 immunoprecipitated from HCT116 lysate by CCK4 Mouse mAb (Ab093426) Lane 3: The isotype control antibody instead of CCK4 Mouse mAb (Ab093426) in HCT116 lysate. Result: CCK4 Mouse mAb (Ab093426) can immunoprecipitate PTK7.