CD105 Mouse mAb - Primary antibody, specific to CD105, IgG1

    Application:
  • ELISA
  • IF/ICC
  • IHC
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
  • Clone Number: 3A9
  • Conjugation: Unconjugated
Item Number
Ab093671
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab093671-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab093671-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$200.90
Ab093671-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$309.90
Ab093671-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,624.90

mAb (3A9); Mouse anti Human CD105 Antibody; WB, IHC, Flow, ELISA, ICC, IF; Unconjugated

Basic Description

Product NameCD105 Mouse mAb - Primary antibody, specific to CD105, IgG1
SynonymsAI528660 antibody | AI662476 antibody | CD 105 antibody | CD105 antibody | CD105 antigen antibody | EGLN_HUMAN antibody | END antibody | Endoglin antibody | Eng antibody | FLJ41744 antibody | HHT1 antibody | ORW antibody | ORW1 antibody | Osler Rendu Webe
Specifications & PurityExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host speciesMouse
SpecificityCD105
ImmunogenPurified recombinant fragment of Human CD105, expressed in E. coli
Positive ControlWB: HUVEC cell lysate. ICC/IF: Hep G2 cells. Flow: Hep G2 cells. IHC: Human kidney cancer and stomach cancer tissues.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Mouse monoclonal (3A9) to CD105, could be used for WB, IHC, Flow, ICC, IF and ELISA.
Application:
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC/IF: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000

Protein Function:
ENG encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia,also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. 

Product Properties

IsotypeIgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStorage: 4℃; -20℃ for long term storage. Avoid repeated freeze/thaw cycles.

Images

CD105 Mouse mAb (Ab093671) - Western Blot
All lanes: CD105 Mouse mAb (Ab093671) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 71 kDa
Observed band size: 93 kDa

CD105 Mouse mAb (Ab093671) - ICC/IF
Immunofluorescence analysis of Hep G2 cells using CD105 Mouse mAb (Ab093671) at a 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

CD105 Mouse mAb (Ab093671) - Flow Cytometry
Flow cytometric analysis of Hep G2 cells using CD105 Mouse mAb (Ab093671)
at a dilution of 1/200 (green) and negative control (purple).

CD105 Mouse mAb (Ab093671) - IHC
Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissues using CD105 Mouse mAb (Ab093671) at a dilution of 1/200 with DAB staining.

CD105 Mouse mAb (Ab093671) - IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using CD105 Mouse mAb (Ab093671) at a dilution of 1/200 with DAB staining.

Associated Targets(Human)

ENG Tbio Endoglin (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/2000

">

1/500-1/2000

IF/ICC

1/200 - 1/1000

ELISA 1/10000

">

1/10000

IHC

1/200-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0800758Certificate of AnalysisAug 03, 2023 Ab093671
ZJ23F0800759Certificate of AnalysisAug 03, 2023 Ab093671

Related Documents

References

1. Cheifetz, S S and 6 more authors..  (1992)  Endoglin is a component of the transforming growth factor-beta receptor system in human endothelial cells..  The Journal of biological chemistry,    (25):   [PMID:1326540]
2. Gougos, A A and Letarte, M M..  (1990)  Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells..  The Journal of biological chemistry,    (25):   [PMID:1692830]
3. McAllister, K A KA and 9 more authors..  (1994)  Endoglin, a TGF-beta binding protein of endothelial cells, is the gene for hereditary haemorrhagic telangiectasia type 1..  Nature genetics,      [PMID:7894484]
4. Bellón, T T and 9 more authors..  (1993)  Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions..  European journal of immunology,      [PMID:8370410]
5. Yamaguchi, H H, Azuma, H H, Shigekiyo, T T, Inoue, H H and Saito, S S..  (1997)  A novel missense mutation in the endoglin gene in hereditary hemorrhagic telangiectasia..  Thrombosis and haemostasis,      [PMID:9157574]
6. Shovlin, C L CL, Hughes, J M JM, Scott, J J, Seidman, C E CE and Seidman, J G JG..  (1997)  Characterization of endoglin and identification of novel mutations in hereditary hemorrhagic telangiectasia..  American journal of human genetics,      [PMID:9245986]
7. Gallione, C J CJ and 18 more authors..  (1998)  Mutation and expression analysis of the endoglin gene in hereditary hemorrhagic telangiectasia reveals null alleles..  Human mutation,      [PMID:9554745]
8. Pece-Barbara, N N, Cymerman, U U, Vera, S S, Marchuk, D A DA and Letarte, M M..  (1999)  Expression analysis of four endoglin missense mutations suggests that haploinsufficiency is the predominant mechanism for hereditary hemorrhagic telangiectasia type 1..  Human molecular genetics,      [PMID:10545596]
9. Cymerman, U U and 6 more authors..  (2000)  Identification of hereditary hemorrhagic telangiectasia type 1 in newborns by protein expression and mutation analysis of endoglin..  Pediatric research,      [PMID:10625079]
10. Gallione, C J CJ and 6 more authors..  (2000)  Two common endoglin mutations in families with hereditary hemorrhagic telangiectasia in the Netherlands Antilles: evidence for a founder effect..  Human genetics,      [PMID:10982033]
11. Lesca, Gaëtan G and 15 more authors..  (2004)  Molecular screening of ALK1/ACVRL1 and ENG genes in hereditary hemorrhagic telangiectasia in France..  Human mutation,      [PMID:15024723]
12. Humphray, S J SJ and 147 more authors..  (2004)  DNA sequence and analysis of human chromosome 9..  Nature,    (27):   [PMID:15164053]
13. Kuehl, Heidi K A HK and 6 more authors..  (2005)  Hepatic manifestation is associated with ALK1 in hereditary hemorrhagic telangiectasia: identification of five novel ALK1 and one novel ENG mutations..  Human mutation,      [PMID:15712270]
14. Argyriou, Loukas L and 6 more authors..  (2006)  Novel mutations in the ENG and ACVRL1 genes causing hereditary hemorrhagic teleangiectasia..  International journal of molecular medicine,      [PMID:16525724]
15. Bossler, Aaron D AD, Richards, Jennifer J, George, Cicily C, Godmilow, Lynn L and Ganguly, Arupa A..  (2006)  Novel mutations in ENG and ACVRL1 identified in a series of 200 individuals undergoing clinical genetic testing for hereditary hemorrhagic telangiectasia (HHT): correlation of genotype with phenotype..  Human mutation,      [PMID:16752392]
16. Meng, Qingjun Q and 10 more authors..  (2006)  Identification of Tctex2beta, a novel dynein light chain family member that interacts with different transforming growth factor-beta receptors..  The Journal of biological chemistry,    (1):   [PMID:16982625]
17. Lee, Nam Y NY and Blobe, Gerard C GC..  (2007)  The interaction of endoglin with beta-arrestin2 regulates transforming growth factor-beta-mediated ERK activation and migration in endothelial cells..  The Journal of biological chemistry,    (20):   [PMID:17540773]
18. Richards-Yutz, Jennifer J, Grant, Kathleen K, Chao, Elizabeth C EC, Walther, Susan E SE and Ganguly, Arupa A..  (2010)  Update on molecular diagnosis of hereditary hemorrhagic telangiectasia..  Human genetics,      [PMID:20414677]
19. Castonguay, Roselyne R and 11 more authors..  (2011)  Soluble endoglin specifically binds bone morphogenetic proteins 9 and 10 via its orphan domain, inhibits blood vessel formation, and suppresses tumor growth..  The Journal of biological chemistry,    (26):   [PMID:21737454]
20. Alt, Aaron A and 8 more authors..  (2012)  Structural and functional insights into endoglin ligand recognition and binding..  PloS one,      [PMID:22347366]
21. Nolan-Stevaux, Olivier O and 6 more authors..  (2012)  Endoglin requirement for BMP9 signaling in endothelial cells reveals new mechanism of action for selective anti-endoglin antibodies..  PloS one,      [PMID:23300529]
22. Saito, Takako T and 7 more authors..  (2017)  Structural Basis of the Human Endoglin-BMP9 Interaction: Insights into BMP Signaling and HHT1..  Cell reports,    (30):   [PMID:28564608]

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