mAb (3A9); Mouse anti Human CD105 Antibody; WB, IHC, Flow, ELISA, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab093671-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab093671-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $200.90 | |
| Ab093671-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $309.90 | |
| Ab093671-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,624.90 |
mAb (3A9); Mouse anti Human CD105 Antibody; WB, IHC, Flow, ELISA, ICC, IF; Unconjugated
| Product Name | CD105 Mouse mAb - Primary antibody, specific to CD105, IgG1 |
|---|---|
| Synonyms | AI528660 antibody; AI662476 antibody; CD 105 antibody; CD105 antibody; CD105 antigen antibody; EGLN_HUMAN antibody; END antibody; Endoglin antibody; Eng antibody; FLJ41744 antibody; HHT1 antibody; ORW antibody; ORW1 antibody; Osler Rendu Weber syndrome 1 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | CD105 |
| Immunogen | Purified recombinant fragment of Human CD105, expressed in E. coli |
| Positive Control | WB: HUVEC cell lysate. ICC/IF: Hep G2 cells. Flow: Hep G2 cells. IHC: Human kidney cancer and stomach cancer tissues. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse monoclonal (3A9) to CD105, could be used for WB, IHC, Flow, ICC, IF and ELISA. |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Storage: 4℃; -20℃ for long term storage. Avoid repeated freeze/thaw cycles. |
CD105 Mouse mAb (Ab093671) - Western Blot
All lanes: CD105 Mouse mAb (Ab093671) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 71 kDa
Observed band size: 93 kDa
CD105 Mouse mAb (Ab093671) - ICC/IF
Immunofluorescence analysis of Hep G2 cells using CD105 Mouse mAb (Ab093671) at a 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
CD105 Mouse mAb (Ab093671) - Flow Cytometry
Flow cytometric analysis of Hep G2 cells using CD105 Mouse mAb (Ab093671)
at a dilution of 1/200 (green) and negative control (purple).
CD105 Mouse mAb (Ab093671) - IHC
Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissues using CD105 Mouse mAb (Ab093671) at a dilution of 1/200 with DAB staining.
CD105 Mouse mAb (Ab093671) - IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using CD105 Mouse mAb (Ab093671) at a dilution of 1/200 with DAB staining.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| ICC/IF |
1/200 - 1/1000
|
| ELISA |
1/10000
|
| IHC |
1/200-1/1000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800758 | Certificate of Analysis | Aug 03, 2023 | Ab093671 |
| ZJ23F0800759 | Certificate of Analysis | Aug 03, 2023 | Ab093671 |
| 1. Cheifetz, S S and 6 more authors.. (1992) Endoglin is a component of the transforming growth factor-beta receptor system in human endothelial cells.. The Journal of biological chemistry, (25): [PMID:1326540] |
| 2. Gougos, A A and Letarte, M M.. (1990) Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.. The Journal of biological chemistry, (25): [PMID:1692830] |
| 3. McAllister, K A KA and 9 more authors.. (1994) Endoglin, a TGF-beta binding protein of endothelial cells, is the gene for hereditary haemorrhagic telangiectasia type 1.. Nature genetics, [PMID:7894484] |
| 4. Bellón, T T and 9 more authors.. (1993) Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions.. European journal of immunology, [PMID:8370410] |
| 5. Yamaguchi, H H, Azuma, H H, Shigekiyo, T T, Inoue, H H and Saito, S S.. (1997) A novel missense mutation in the endoglin gene in hereditary hemorrhagic telangiectasia.. Thrombosis and haemostasis, [PMID:9157574] |
| 6. Shovlin, C L CL, Hughes, J M JM, Scott, J J, Seidman, C E CE and Seidman, J G JG.. (1997) Characterization of endoglin and identification of novel mutations in hereditary hemorrhagic telangiectasia.. American journal of human genetics, [PMID:9245986] |
| 7. Gallione, C J CJ and 18 more authors.. (1998) Mutation and expression analysis of the endoglin gene in hereditary hemorrhagic telangiectasia reveals null alleles.. Human mutation, [PMID:9554745] |
| 8. Pece-Barbara, N N, Cymerman, U U, Vera, S S, Marchuk, D A DA and Letarte, M M.. (1999) Expression analysis of four endoglin missense mutations suggests that haploinsufficiency is the predominant mechanism for hereditary hemorrhagic telangiectasia type 1.. Human molecular genetics, [PMID:10545596] |
| 9. Cymerman, U U and 6 more authors.. (2000) Identification of hereditary hemorrhagic telangiectasia type 1 in newborns by protein expression and mutation analysis of endoglin.. Pediatric research, [PMID:10625079] |
| 10. Gallione, C J CJ and 6 more authors.. (2000) Two common endoglin mutations in families with hereditary hemorrhagic telangiectasia in the Netherlands Antilles: evidence for a founder effect.. Human genetics, [PMID:10982033] |
| 11. Lesca, Gaëtan G and 15 more authors.. (2004) Molecular screening of ALK1/ACVRL1 and ENG genes in hereditary hemorrhagic telangiectasia in France.. Human mutation, [PMID:15024723] |
| 12. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): [PMID:15164053] |
| 13. Kuehl, Heidi K A HK and 6 more authors.. (2005) Hepatic manifestation is associated with ALK1 in hereditary hemorrhagic telangiectasia: identification of five novel ALK1 and one novel ENG mutations.. Human mutation, [PMID:15712270] |
| 14. Argyriou, Loukas L and 6 more authors.. (2006) Novel mutations in the ENG and ACVRL1 genes causing hereditary hemorrhagic teleangiectasia.. International journal of molecular medicine, [PMID:16525724] |
| 15. Bossler, Aaron D AD, Richards, Jennifer J, George, Cicily C, Godmilow, Lynn L and Ganguly, Arupa A.. (2006) Novel mutations in ENG and ACVRL1 identified in a series of 200 individuals undergoing clinical genetic testing for hereditary hemorrhagic telangiectasia (HHT): correlation of genotype with phenotype.. Human mutation, [PMID:16752392] |
| 16. Meng, Qingjun Q and 10 more authors.. (2006) Identification of Tctex2beta, a novel dynein light chain family member that interacts with different transforming growth factor-beta receptors.. The Journal of biological chemistry, (1): [PMID:16982625] |
| 17. Lee, Nam Y NY and Blobe, Gerard C GC.. (2007) The interaction of endoglin with beta-arrestin2 regulates transforming growth factor-beta-mediated ERK activation and migration in endothelial cells.. The Journal of biological chemistry, (20): [PMID:17540773] |
| 18. Richards-Yutz, Jennifer J, Grant, Kathleen K, Chao, Elizabeth C EC, Walther, Susan E SE and Ganguly, Arupa A.. (2010) Update on molecular diagnosis of hereditary hemorrhagic telangiectasia.. Human genetics, [PMID:20414677] |
| 19. Castonguay, Roselyne R and 11 more authors.. (2011) Soluble endoglin specifically binds bone morphogenetic proteins 9 and 10 via its orphan domain, inhibits blood vessel formation, and suppresses tumor growth.. The Journal of biological chemistry, (26): [PMID:21737454] |
| 20. Alt, Aaron A and 8 more authors.. (2012) Structural and functional insights into endoglin ligand recognition and binding.. PloS one, [PMID:22347366] |
| 21. Nolan-Stevaux, Olivier O and 6 more authors.. (2012) Endoglin requirement for BMP9 signaling in endothelial cells reveals new mechanism of action for selective anti-endoglin antibodies.. PloS one, [PMID:23300529] |
| 22. Saito, Takako T and 7 more authors.. (2017) Structural Basis of the Human Endoglin-BMP9 Interaction: Insights into BMP Signaling and HHT1.. Cell reports, (30): [PMID:28564608] |
