| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab093718-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab093718-50μg | 50μg | In stock | $169.90 | |
| Ab093718-100μg | 100μg | In stock | $299.90 | |
| Ab093718-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $519.90 |
mAb (M1/70); Rat anti Human/Mouse CD11b Antibody; Flow Cytometry; Unconjugated
| Product Name | CD11b Rat mAb - Primary antibody, specific to CD11b, Rat IgG2b |
|---|---|
| Synonyms | antigen CD11b (p170) | CD11 antigen-like family member B | CD11b antigen | CD11b | Cell surface glycoprotein MAC-1 subunit alpha | Complement component 3 receptor 3 subunit | CR-3 alpha chain | CR3A | CR3AMGC117044 | Integrin alpha M | integrin alpha-M | |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Biochemical and Physiological Mechanisms | Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third |
| Host species | Rat |
| Specificity | CD11b |
| Immunogen | C57BL/10 splenocytes |
| Positive Control | Flow Cytometry: Human peripheral blood granulocyte |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description |
Rat anti Human/Mouse CD11b Antibody, Monoclonal (M1/70), could be used for Flow Cytometry and so on.
|
| Isotype | Rat IgG2b |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot. |
CD11b Rat mAb (Ab093718) - Flow Cytometry
Flow Cytometry analysis of THP-1 cells labelling CD11b (red) with CD11b Rat mAb (Ab093718) at 0.1 μg/mL for 1 hour at 4°C. Goat anti-Rat IgG (H+L) Alexa Fluor 488 at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | 1μg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505988 | Certificate of Analysis | May 23, 2024 | Ab093718 |
| ZJ24F0505987 | Certificate of Analysis | May 23, 2024 | Ab093718 |
| ZJ23F1001481 | Certificate of Analysis | Nov 02, 2023 | Ab093718 |
| ZJ23F1001482 | Certificate of Analysis | Nov 02, 2023 | Ab093718 |
| 1. Faridi MH, Maiguel D, Barth CJ, Stoub D, Day R, Schürer S, Gupta V.. (2009) Identification of novel agonists of the integrin CD11b/CD18.. Bioorg Med Chem Lett, 19 (24): (6902-6906). [PMID:19879752] |
| 2. Chen J, Song Y, Bojadzic D, Tamayo-Garcia A, Landin AM, Blomberg BB, Buchwald P.. (2017) Small-Molecule Inhibitors of the CD40-CD40L Costimulatory Protein-Protein Interaction.. J Med Chem, 60 (21): (8906-8922). [PMID:29024591] |
| 3. Pahl, H L HL, Rosmarin, A G AG and Tenen, D G DG.. (1992) Characterization of the myeloid-specific CD11b promoter.. Blood, (15): [PMID:1346576] |
| 4. Shelley, C S CS and Arnaout, M A MA.. (1991) The promoter of the CD11b gene directs myeloid-specific and developmentally regulated expression.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1683702] |
| 5. Arnaout, M A MA, Gupta, S K SK, Pierce, M W MW and Tenen, D G DG.. (1988) Amino acid sequence of the alpha subunit of human leukocyte adhesion receptor Mo1 (complement receptor type 3).. The Journal of cell biology, [PMID:2454931] |
| 6. Corbi, A L AL, Kishimoto, T K TK, Miller, L J LJ and Springer, T A TA.. (1988) The human leukocyte adhesion glycoprotein Mac-1 (complement receptor type 3, CD11b) alpha subunit. Cloning, primary structure, and relation to the integrins, von Willebrand factor and factor B.. The Journal of biological chemistry, (5): [PMID:2457584] |
| 7. Hickstein, D D DD and 5 more authors.. (1989) cDNA sequence for the alpha M subunit of the human neutrophil adherence receptor indicates homology to integrin alpha subunits.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2563162] |
| 8. Arnaout, M A MA, Remold-O'Donnell, E E, Pierce, M W MW, Harris, P P and Tenen, D G DG.. (1988) Molecular cloning of the alpha subunit of human and guinea pig leukocyte adhesion glycoprotein Mo1: chromosomal localization and homology to the alpha subunits of integrins.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2833753] |
| 9. Pierce, M W MW, Remold-O'Donnell, E E, Todd, R F RF and Arnaout, M A MA.. (1986) N-terminal sequence of human leukocyte glycoprotein Mo1: conservation across species and homology to platelet IIb/IIIa.. Biochimica et biophysica acta, (12): [PMID:3539202] |
| 10. Lee, J O JO, Rieu, P P, Arnaout, M A MA and Liddington, R R.. (1995) Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18).. Cell, (24): [PMID:7867070] |
| 11. Fleming, J C JC, Pahl, H L HL, Gonzalez, D A DA, Smith, T F TF and Tenen, D G DG.. (1993) Structural analysis of the CD11b gene and phylogenetic analysis of the alpha-integrin gene family demonstrate remarkable conservation of genomic organization and suggest early diversification during evolution.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:8419480] |
| 12. Lee, J O JO, Bankston, L A LA, Arnaout, M A MA and Liddington, R C RC.. (1995) Two conformations of the integrin A-domain (I-domain): a pathway for activation?. Structure (London, England : 1993), (15): [PMID:8747460] |
| 13. DiScipio, R G RG, Daffern, P J PJ, Schraufstätter, I U IU and Sriramarao, P P.. (1998) Human polymorphonuclear leukocytes adhere to complement factor H through an interaction that involves alphaMbeta2 (CD11b/CD18).. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:9558116] |
| 14. Oxvig, C C and Springer, T A TA.. (1998) Experimental support for a beta-propeller domain in integrin alpha-subunits and a calcium binding site on its lower surface.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:9560195] |
| 15. Baldwin, E T ET and 16 more authors.. (1998) Cation binding to the integrin CD11b I domain and activation model assessment.. Structure (London, England : 1993), (15): [PMID:9687375] |
| 16. Santoso, Sentot S and 6 more authors.. (2002) The junctional adhesion molecule 3 (JAM-3) on human platelets is a counterreceptor for the leukocyte integrin Mac-1.. The Journal of experimental medicine, (2): [PMID:12208882] |
| 17. Zen, Ke K and 5 more authors.. (2004) JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration.. Molecular biology of the cell, [PMID:15194813] |
| 18. . (2008) Genome-wide association scan in women with systemic lupus erythematosus identifies susceptibility variants in ITGAM, PXK, KIAA1542 and other loci.. Nature genetics, [PMID:18204446] |
| 19. Nath, Swapan K SK and 18 more authors.. (2008) A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus.. Nature genetics, [PMID:18204448] |
| 20. Losse, Josephine J, Zipfel, Peter F PF and Józsi, Mihály M.. (2010) Factor H and factor H-related protein 1 bind to human neutrophils via complement receptor 3, mediate attachment to Candida albicans, and enhance neutrophil antimicrobial activity.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:20008295] |
| 21. Jerke, Uwe U and 7 more authors.. (2011) Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation.. The Journal of biological chemistry, (4): [PMID:21193407] |
| 22. Kawamoto, Eiji E and 6 more authors.. (2016) LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin.. Biochemical and biophysical research communications, (13): [PMID:27055590] |
| 23. Bai, Ming M and 13 more authors.. (2017) CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation.. Blood, (9): [PMID:28807980] |
