CD14 Mouse mAb (AF405)

    Application:
  • Flow Cytometry
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG2a
  • Clone Number: UCHM-1
  • Conjugation: Alexa Fluor® 405
Item Number
Ab210084
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab210084-10T (Trial Size)
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10T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$62.90
Ab210084-25T
25T
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Production requires sourcing of materials. We appreciate your patience and understanding.
$125.90
Ab210084-100T
100T
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Production requires sourcing of materials. We appreciate your patience and understanding.
$315.90

mAb (UCHM-1); Mouse anti Human CD14 Antibody (AF405); Flow Cytometry

Basic Description

Product NameCD14 Mouse mAb (AF405)
Specifications & PurityExactAb™, Validated, Ex:401nm, Em:422nm, 5 μL/test
Biochemical and Physiological MechanismsCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molec
Host speciesMouse
SpecificityCD14
ImmunogenHuman Thymocytes followed by peripheral blood mononuclear cells
Positive ControlFlow: Human peripheral blood monocytes
ConjugationAlexa Fluor® 405
GradeEm:422nm, Ex:401nm, ExactAb™, Validated
Product Description

Mouse anti Human CD14 Antibody, Monoclonal (UCHM-1), could be used for Flow.

Product Properties

Light Chain Typekappa
Purification MethodProtein G purified
Concentration5 μL/test
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

CD14 Mouse mAb (AF405) (Ab210084) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood  (right panel) compared to an isotype control (left panel). Cells were stained with CD14 Mouse mAb (AF405) (Ab210084) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF405) (Ab190076).

CD14 Mouse mAb (AF405) (Ab210084) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with CD14 Mouse mAb (AF405) (Ab210084) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

CD14 Tbio Monocyte differentiation antigen CD14 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
Flow Cytometry 5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. ">

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates

Certificate of Analysis(COA)

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Related Documents

Solution Calculators