mAb (3G8); Mouse anti Human CD16 Antibody; Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab093973-10μg | 10μg | In stock | $26.90 | |
| Ab093973-50μg | 50μg | In stock | $79.90 | |
| Ab093973-100μg | 100μg | In stock | $109.90 | |
| Ab093973-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $899.90 |
mAb (3G8); Mouse anti Human CD16 Antibody; Flow; Unconjugated
| Product Name | CD16 Mouse mAb - Primary antibody, specific to CD16, Mouse IgG1 |
|---|---|
| Synonyms | CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20; CD 16 antibody; CD 16a antibody; CD16 antibody; CD16a antibody; CD16a antigen antibody; CD16B antibody; CD16b antigen antibody; Fc f |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Lot by Lot |
| Host species | Mouse |
| Specificity | CD16 |
| Immunogen | Human polymorphonuclear leukocytes. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human CD16 Antibody, Monoclonal (3G8), could be used for Flow and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot. |
CD16 Mouse mAb (Ab093973) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD16 with CD16 Mouse mAb (Ab093973) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
CD16 Mouse mAb (Ab093973) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD16 (red) with CD16 Mouse mAb (Ab093973) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | < 0.5µg/100w cells |
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202886 | Certificate of Analysis | Mar 01, 2024 | Ab093973 |
| ZJ24F0202885 | Certificate of Analysis | Mar 01, 2024 | Ab093973 |
| ZJ24F0202884 | Certificate of Analysis | Mar 01, 2024 | Ab093973 |
| 1. da Silva AJ, Brickelmaier M, Majeau GR, Li Z, Su L, Hsu YM, Hochman PS. (2002) Alefacept, an immunomodulatory recombinant LFA-3/IgG1 fusion protein, induces CD16 signaling and CD2/CD16-dependent apoptosis of CD2(+) cells.. J Immunol, 168 (9): (4462-71). [PMID:11970990] |
| 2. Ghazi A, Trikha A, Calhoun WJ. (2012) Benralizumab--a humanized mAb to IL-5Rα with enhanced antibody-dependent cell-mediated cytotoxicity--a novel approach for the treatment of asthma.. Expert Opin Biol Ther, 12 (1): (113-8). [PMID:22136436] |
| 3. Reusch U, Burkhardt C, Fucek I, Le Gall F, Le Gall M, Hoffmann K, Knackmuss SH, Kiprijanov S, Little M, Zhukovsky EA. (2014) A novel tetravalent bispecific TandAb (CD30/CD16A) efficiently recruits NK cells for the lysis of CD30+ tumor cells.. MAbs, 6 (3): (728-39). [PMID:24670809] |
| 4. Xiang H, Liu L, Gao Y, Ahene A, Macal M, Hsu AW, Dreiling L, Collins H. (2020) Population pharmacokinetic analysis of phase 1 bemarituzumab data to support phase 2 gastroesophageal adenocarcinoma FIGHT trial.. Cancer Chemother Pharmacol, 86 (5): (595-606). [PMID:32965540] |
| 5. Wei Hseun Yeap,Kok Loon Wong,Noriko Shimasaki,Esmeralda Chi Yuan Teo,Jeffrey Kim Siang Quek,Hao Xiang Yong,Colin Phipps Diong,Antonio Bertoletti,Yeh Ching Linn,Siew Cheng Wong. (2016-09-28) CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes.. Scientific reports, 6 (34310-34310). [PMID:27670158] |
| 6. Scallon, B J BJ and 6 more authors.. (1989) A human immunoglobulin G receptor exists in both polypeptide-anchored and phosphatidylinositol-glycan-anchored forms.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2525780] |
| 7. Ravetch, J V JV and Perussia, B B.. (1989) Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions.. The Journal of experimental medicine, (1): [PMID:2526846] |
| 8. Morcos, M M and 5 more authors.. (1994) Human glomerular mesangial cells express CD16 and may be stimulated via this receptor.. Kidney international, [PMID:7700021] |
| 9. Gessner, J E JE, Grussenmeyer, T T, Kolanus, W W and Schmidt, R E RE.. (1995) The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions.. The Journal of biological chemistry, (20): [PMID:7836402] |
| 10. Jawahar, S and 5 more authors.. (1996) Natural Killer (NK) cell deficiency associated with an epitope-deficient Fc receptor type IIIA (CD16-II).. Clinical and experimental immunology, [PMID:8608639] |
| 11. de Haas, M M and 7 more authors.. (1996) A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:8609432] |
| 12. Koene, H R HR and 5 more authors.. (1997) Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype.. Blood, (1): [PMID:9242542] |
| 13. Wu, J J and 7 more authors.. (1997) A novel polymorphism of FcgammaRIIIa (CD16) alters receptor function and predisposes to autoimmune disease.. The Journal of clinical investigation, (1): [PMID:9276722] |
| 14. Warren, H S HS and Kinnear, B F BF.. (1999) Quantitative analysis of the effect of CD16 ligation on human NK cell proliferation.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:9916693] |
| 15. Vaishnaw, Ashay K AK and TenHoor, Christopher N CN.. (2002) Pharmacokinetics, biologic activity, and tolerability of alefacept by intravenous and intramuscular administration.. Journal of pharmacokinetics and pharmacodynamics, [PMID:12795239] |
| 16. Lin, Thomas S TS and 9 more authors.. (2005) FCGR3A and FCGR2A polymorphisms may not correlate with response to alemtuzumab in chronic lymphocytic leukemia.. Blood, (1): [PMID:15217834] |
| 17. Criswell, Lindsey A LA and 11 more authors.. (2004) The influence of genetic variation in the HLA-DRB1 and LTA-TNF regions on the response to treatment of early rheumatoid arthritis with methotrexate or etanercept.. Arthritis and rheumatism, [PMID:15457442] |
| 18. Hughes, L B LB and 7 more authors.. (2004) Genetic risk factors for infection in patients with early rheumatoid arthritis.. Genes and immunity, [PMID:15526004] |
| 19. Wang, Yaning Y, Fei, David D, Vanderlaan, Martin M and Song, An A.. (2004) Biological activity of bevacizumab, a humanized anti-VEGF antibody in vitro.. Angiogenesis, [PMID:15886877] |
| 20. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 21. Zhang, Wu W and 12 more authors.. (2007) FCGR2A and FCGR3A polymorphisms associated with clinical outcome of epidermal growth factor receptor expressing metastatic colorectal cancer patients treated with single-agent cetuximab.. Journal of clinical oncology : official journal of the American Society of Clinical Oncology, (20): [PMID:17704420] |
| 22. Seimetz, Diane D, Lindhofer, Horst H and Bokemeyer, Carsten C.. (2010) Development and approval of the trifunctional antibody catumaxomab (anti-EpCAM x anti-CD3) as a targeted cancer immunotherapy.. Cancer treatment reviews, [PMID:20347527] |
| 23. Ferrara, Claudia C and 11 more authors.. (2011) Unique carbohydrate-carbohydrate interactions are required for high affinity binding between FcgammaRIII and antibodies lacking core fucose.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:21768335] |
| 24. Mizushima, Tsunehiro and 8 more authors.. (2011) Structural basis for improved efficacy of therapeutic antibodies on defucosylation of their Fc glycans.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:22023369] |
| 25. Grier, Jennifer T JT and 8 more authors.. (2012) Human immunodeficiency-causing mutation defines CD16 in spontaneous NK cell cytotoxicity.. The Journal of clinical investigation, [PMID:23006327] |
| 26. Li, Xiaoli X and 7 more authors.. (2012) The unique cytoplasmic domain of human FcγRIIIA regulates receptor-mediated function.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:23024279] |
| 27. Laviolette, Michel and 11 more authors.. (2013) Effects of benralizumab on airway eosinophils in asthmatic patients with sputum eosinophilia.. The Journal of allergy and clinical immunology, [PMID:23866823] |
| 28. Lajoie, Laurie L and 10 more authors.. (2014) ADAM17-mediated shedding of FcγRIIIA on human NK cells: identification of the cleavage site and relationship with activation.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:24337742] |
| 29. DiLillo, David J DJ, Tan, Gene S GS, Palese, Peter P and Ravetch, Jeffrey V JV.. (2014) Broadly neutralizing hemagglutinin stalk-specific antibodies require FcγR interactions for protection against influenza virus in vivo.. Nature medicine, [PMID:24412922] |
| 30. Lee, Jaewon J and 9 more authors.. (2015) Epigenetic modification and antibody-dependent expansion of memory-like NK cells in human cytomegalovirus-infected individuals.. Immunity, (17): [PMID:25786175] |
| 31. Jing, Yawu Y and 6 more authors.. (2015) Identification of an ADAM17 cleavage region in human CD16 (FcγRIII) and the engineering of a non-cleavable version of the receptor in NK cells.. PloS one, [PMID:25816339] |
| 32. Grugan, Katharine D KD and 7 more authors.. (2017) Fc-mediated activity of EGFR x c-Met bispecific antibody JNJ-61186372 enhanced killing of lung cancer cells.. mAbs, [PMID:27786612] |
| 33. Wang, B; Yan, L; Yao, Z and Roskos, L K.. (2017) Population Pharmacokinetics and Pharmacodynamics of Benralizumab in Healthy Volunteers and Patients With Asthma.. CPT: pharmacometrics & systems pharmacology, [PMID:28109128] |
| 34. Wang, Taia T and 12 more authors.. (2017) IgG antibodies to dengue enhanced for FcγRIIIA binding determine disease severity.. Science (New York, N.Y.), (27): [PMID:28126818] |
| 35. Blázquez-Moreno, Alfonso A and 5 more authors.. (2017) Transmembrane features governing Fc receptor CD16A assembly with CD16A signaling adaptor molecules.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:28652325] |
