CD2 Mouse mAb - Primary antibody, specific to CD2, Mouse IgG1

Features and benefits
  • Short Overview:

    mAb (RPA-2.10); Mouse anti Human CD2 Antibody; Flow; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
    Application:
  • Flow Cytometry
Item Number
Ab006467
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab006467-10μg
10μg
In stock
$16.90
Ab006467-50μg
50μg
In stock
$49.90
Ab006467-100μg
100μg
In stock
$79.90
Ab006467-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$649.90

mAb (RPA-2.10); Mouse anti Human CD2 Antibody; Flow; Unconjugated

Basic Description

Product NameCD2 Mouse mAb - Primary antibody, specific to CD2, Mouse IgG1
SynonymsCD2 antigen (p50), sheep red blood cell receptor; CD2 antigen; CD2 molecule; CD2; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host speciesMouse
SpecificityCD2
ImmunogenHuman phytohemagglutinin-treated lymphoblasts
Positive ControlFlow: Human peripheral blood lymphocytes.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human CD2 Antibody, Monoclonal (RPA-2.10), could be used for Flow and so on.
Application:
Flow: 1μg/100w cells
Protein Function
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at 2-8°C
Shipped InWet ice
Stability And StorageShipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot.

Images

CD2 Mouse mAb (Ab006467) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD2 with CD2 Mouse mAb (Ab006467) at 0.1 μg/mL (right panel) compared with mouse IgG (Ab170221) - Isotype Control (left panel). Goat anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 was used as the secondary antibody.

CD2 Mouse mAb (Ab006467) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD2 (red) with CD2 Mouse mAb (Ab006467) at 0.1 μg/mL for 1 hour at 4°C. Goat anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

CD2 Tclin T-cell surface antigen CD2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
CD2 Tclin T-cell surface antigen CD2 (9 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CD2 Tclin Lymphocyte function-associated antigen 3/T-cell surface antigen CD2 (9 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
Flow Cytometry

1μg/100w cells

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0102388Certificate of AnalysisJan 10, 2024 Ab006467
ZJ24F0102387Certificate of AnalysisJan 10, 2024 Ab006467
ZJ24F0102386Certificate of AnalysisJan 10, 2024 Ab006467

Related Documents

References

1. da Silva AJ, Brickelmaier M, Majeau GR, Li Z, Su L, Hsu YM, Hochman PS.  (2002)  Alefacept, an immunomodulatory recombinant LFA-3/IgG1 fusion protein, induces CD16 signaling and CD2/CD16-dependent apoptosis of CD2(+) cells..  J Immunol,  168  (9): (4462-71).  [PMID:11970990]
2. Giddu S, Subramanian V, Yoon HS, Satyanarayanajois SD..  (2009)  Design of beta-hairpin peptides for modulation of cell adhesion by beta-turn constraint..  J Med Chem,  52  (3): (726-736).  [PMID:19123855]
3. Gokhale A, Weldeghiorghis TK, Taneja V, Satyanarayanajois SD..  (2011)  Conformationally constrained peptides from CD2 to modulate protein-protein interactions between CD2 and CD58..  J Med Chem,  54  (15): (5307-5319).  [PMID:21755948]
4. Hahn, W C WC, Menu, E E, Bothwell, A L AL, Sims, P J PJ and Bierer, B E BE..  (1992)  Overlapping but nonidentical binding sites on CD2 for CD58 and a second ligand CD59..  Science (New York, N.Y.),    (26):   [PMID:1377404]
5. Seed, B B and Aruffo, A A..  (1987)  Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2437578]
6. Peterson, A A and Seed, B B..  (1987)  Monoclonal antibody and ligand binding sites of the T cell erythrocyte receptor (CD2)..  Nature,      [PMID:2444890]
7. Sayre, P H PH and 7 more authors..  (1987)  Molecular cloning and expression of T11 cDNAs reveal a receptor-like structure on human T lymphocytes..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2883656]
8. Diamond, D J DJ, Clayton, L K LK, Sayre, P H PH and Reinherz, E L EL..  (1988)  Exon-intron organization and sequence comparison of human and murine T11 (CD2) genes..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2894031]
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10. Sewell, W A WA, Brown, M H MH, Dunne, J J, Owen, M J MJ and Crumpton, M J MJ..  (1986)  Molecular cloning of the human T-lymphocyte surface CD2 (T11) antigen..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3490670]
11. Wyss, D F DF and 8 more authors..  (1995)  Conformation and function of the N-linked glycan in the adhesion domain of human CD2..  Science (New York, N.Y.),    (1):   [PMID:7544493]
12. Withka, J M JM and 5 more authors..  (1993)  Structure of the glycosylated adhesion domain of human T lymphocyte glycoprotein CD2..  Structure (London, England : 1993),    (15):   [PMID:7915183]
13. Bodian, D L DL, Jones, E Y EY, Harlos, K K, Stuart, D I DI and Davis, S J SJ..  (1994)  Crystal structure of the extracellular region of the human cell adhesion molecule CD2 at 2.5 A resolution..  Structure (London, England : 1993),    (15):   [PMID:7994575]
14. Li, J J and 7 more authors..  (1998)  A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion..  The EMBO journal,    (15):   [PMID:9857189]
15. Wang, J H JH and 7 more authors..  (1999)  Structure of a heterophilic adhesion complex between the human CD2 and CD58 (LFA-3) counterreceptors..  Cell,    (11):   [PMID:10380930]
16. Chen, X X, Ji, Z L ZL and Chen, Y Z YZ..  (2002)  TTD: Therapeutic Target Database..  Nucleic acids research,    (1):   [PMID:11752352]
17. and Ortonne, J-P JP..  (2003)  Clinical response to alefacept: results of a phase 3 study of intramuscular administration of alefacept in patients with chronic plaque psoriasis..  Journal of the European Academy of Dermatology and Venereology : JEADV,      [PMID:12795770]
18. Goedkoop, Amber Y AY and 8 more authors..  (2004)  Alefacept therapy reduces the effector T-cell population in lesional psoriatic epidermis..  Archives of dermatological research,      [PMID:14968366]
19. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,    (18):   [PMID:16710414]
20. Sjöblom, Tobias T and 28 more authors..  (2006)  The consensus coding sequences of human breast and colorectal cancers..  Science (New York, N.Y.),    (13):   [PMID:16959974]
21. Larsen, R R, Ryder, L P LP, Svejgaard, A A and Gniadecki, R R..  (2007)  Changes in circulating lymphocyte subpopulations following administration of the leucocyte function-associated antigen-3 (LFA-3)/IgG1 fusion protein alefacept..  Clinical and experimental immunology,      [PMID:17403057]
22. Chamian, Francesca F and 11 more authors..  (2007)  Alefacept (anti-CD2) causes a selective reduction in circulating effector memory T cells (Tem) and relative preservation of central memory T cells (Tcm) in psoriasis..  Journal of translational medicine,    (7):   [PMID:17555598]
23. Grier, Jennifer T JT and 8 more authors..  (2012)  Human immunodeficiency-causing mutation defines CD16 in spontaneous NK cell cytotoxicity..  The Journal of clinical investigation,      [PMID:23006327]

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