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CD23 Antibody - Primary antibody, specific to CD23, Rabbit IgG

  • Azide Free
  • Carrier Free
  • ExactAb™
  • Validated
  • Lot by Lot
Features and benefits
  • Short Overview:

    pAb; Rabbit anti Human CD23 Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: Rabbit IgG
    Application:
  • IHC
  • WB
Item Number
Ab006720
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab006720-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab006720-20μl
20μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$611.90
Ab006720-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$179.90
Ab006720-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$299.90
Ab006720-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$769.90

pAb; Rabbit anti Human CD23 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameCD23 Antibody - Primary antibody, specific to CD23, Rabbit IgG
SynonymsBlast 2 antibody; BLAST-2 antibody; Blast2 antibody; C type lectin domain family 4 member J antibody; C-type lectin domain family 4 antibody; C-type lectin domain family 4 member J antibody; C-type lectin domain family 4, member J antibody; CD 23 antibody
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host speciesRabbit
SpecificityCD23
ImmunogenRecombinant CD23 expressed in E.coli (AA 151-321)
Positive ControlWB: NIH/3T3, Raw264.7 and Raji cell lysates. IHC: Human Tonsil tissue, Human Amygdalitis tissue.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human CD23 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.2-2 µg/mL
IHC: 5-20 µg/mL
Protein Function
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified and protein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

CD23 Antibody (Ab006720) - Western Blot
All lanes: CD23 Antibody (Ab006720) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 36, 40 kDa
Exposure time: 22.9 s

CD23 Antibody (Ab006720) - IHC
DAB staining on IHC
Samples: Human Tonsil Tissue
Primary Ab: 10 µg/mL CD23 Antibody (Ab006720)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

CD23 Antibody (Ab006720) - IHC
DAB staining on IHC
Samples: Human Amygdalitis Tissue
Primary Ab: 20 μg/mL CD23 Antibody (Ab006720)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Associated Targets

FCER2 Tchem Low affinity immunoglobulin epsilon Fc receptor 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

5-20 µg/mL

WB

0.5-2 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001289Certificate of AnalysisOct 13, 2023 Ab006720
ZJ23F1001288Certificate of AnalysisOct 13, 2023 Ab006720

Related Documents

References

1. Yokota A, Kikutani H, Tanaka T, Sato R, Barsumian EL, Suemura M, Kishimoto T.  (1988)  Two species of human Fc epsilon receptor II (Fc epsilon RII/CD23): tissue-specific and IL-4-specific regulation of gene expression..  Cell,  55  (4): (611-8).  [PMID:2972386]
2. Rose, K K and 8 more authors..  (1992)  Partial characterization of natural and recombinant human soluble CD23..  The Biochemical journal,    (15):   [PMID:1417742]
3. Pirron, U U, Schlunck, T T, Prinz, J C JC and Rieber, E P EP..  (1990)  IgE-dependent antigen focusing by human B lymphocytes is mediated by the low-affinity receptor for IgE..  European journal of immunology,      [PMID:2167225]
4. Kikutani, H H and 9 more authors..  (1986)  Molecular structure of human lymphocyte receptor for immunoglobulin E..  Cell,    (5):   [PMID:2877743]
5. Ikuta, K K and 7 more authors..  (1987)  Human lymphocyte Fc receptor for IgE: sequence homology of its cloned cDNA with animal lectins..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2949326]
6. Lüdin, C C and 8 more authors..  (1987)  Cloning and expression of the cDNA coding for a human lymphocyte IgE receptor..  The EMBO journal,      [PMID:3034567]
7. Vouldoukis, I I and 7 more authors..  (1995)  The killing of Leishmania major by human macrophages is mediated by nitric oxide induced after ligation of the Fc epsilon RII/CD23 surface antigen..  Proceedings of the National Academy of Sciences of the United States of America,    (15):   [PMID:7544003]
8. Padlan, E A EA and Helm, B A BA..  (1993)  Modeling of the lectin-homology domains of the human and murine low-affinity Fc epsilon receptor (Fc epsilon RII/CD23)..  Receptor,      [PMID:8142907]
9. Bajorath, J J and Aruffo, A A..  (1996)  Structure-based modeling of the ligand binding domain of the human cell surface receptor CD23 and comparison of two independently derived molecular models..  Protein science : a publication of the Protein Society,      [PMID:8745401]
10. Grimwood, Jane J and 97 more authors..  (2004)  The DNA sequence and biology of human chromosome 19..  Nature,    (1):   [PMID:15057824]
11. Hibbert, Richard G RG and 9 more authors..  (2005)  The structure of human CD23 and its interactions with IgE and CD21..  The Journal of experimental medicine,    (19):   [PMID:16172256]
12. Wurzburg, Beth A BA, Tarchevskaya, Svetlana S SS and Jardetzky, Theodore S TS..  (2006)  Structural changes in the lectin domain of CD23, the low-affinity IgE receptor, upon calcium binding..  Structure (London, England : 1993),      [PMID:16765898]
13. Lemieux, George A GA and 9 more authors..  (2007)  The low affinity IgE receptor (CD23) is cleaved by the metalloproteinase ADAM10..  The Journal of biological chemistry,    (18):   [PMID:17389606]
14. Pathan, Nuzhat I NI and 5 more authors..  (2008)  Mediation of apoptosis by and antitumor activity of lumiliximab in chronic lymphocytic leukemia cells and CD23+ lymphoma cell lines..  Blood,    (1):   [PMID:18032710]
15. Ivaldi, Corinne C and 6 more authors..  (2012)  Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23..  PloS one,      [PMID:22615937]
16. Hersheson, Joshua J and 17 more authors..  (2013)  Mutations in the autoregulatory domain of β-tubulin 4a cause hereditary dystonia..  Annals of neurology,      [PMID:23424103]

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