CD235a/Glycophorin A Mouse mAb - Primary antibody, specific to CD235a/Glycophorin A, Mouse IgG2a

    Application:
  • Flow Cytometry
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG2a
  • Clone Number: HI264
  • Conjugation: Unconjugated
Item Number
Ab105785
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab105785-10μg
10μg
In stock
$19.90
Ab105785-50μg
50μg
In stock
$59.90
Ab105785-100μg
100μg
In stock
$99.90
Ab105785-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$799.90

mAb (HI264); Mouse anti Human CD235a/Glycophorin A Antibody; Flow; Unconjugated

Basic Description

Product NameCD235a/Glycophorin A Mouse mAb - Primary antibody, specific to CD235a/Glycophorin A, Mouse IgG2a
SynonymsMN | CD235a antigen | CD235a | glycophorin A (includes MN blood group) | glycophorin A (MNS blood group) | Glycophorin A | glycophorin Erik | glycophorin MiI | glycophorin MiIII | glycophorin MiV | glycophorin MiX | glycophorin SAT | glycophorin Sta type
Specifications & PurityExactAb™, Validated, Carrier Free, Lot by Lot
Host speciesMouse
SpecificityCD235a/Glycophorin A
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Mouse anti Human CD235a/Glycophorin A Antibody, Monoclonal (HI264), could be used for Flow and so on.
Application:
Flow<0.5µg/100w cells
Protein Function
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).

Product Properties

IsotypeMouse IgG2a
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at 2-8°C
Shipped InWet ice
Stability And StorageStore at 4°C short term (1-2 weeks). Store at 4°C long term (36 months). Upon delivery aliquot.

Images

CD235a/Glycophorin A Mouse mAb (Ab105785) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD235a/Glycophorin A (red) with CD235a/Glycophorin A Mouse mAb (Ab105785) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

GYPA Tbio Glycophorin-A (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
Flow Cytometry

< 0.5µg/100w cells

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0102446Certificate of AnalysisJan 18, 2024 Ab105785
ZJ24F0102445Certificate of AnalysisJan 18, 2024 Ab105785
ZJ24F0102444Certificate of AnalysisJan 18, 2024 Ab105785

Related Documents

References

1. Bruce LJ, Pan RJ, Cope DL, Uchikawa M, Gunn RB, Cherry RJ, Tanner MJ.  ()  Altered structure and anion transport properties of band 3 (AE1, SLC4A1) in human red cells lacking glycophorin A..  J Biol Chem,      [PMID:14604989] [http://pubmed.ncbi.nlm.nih.gov/14604989]
2. Tomita, M M and Marchesi, V T VT..  (1975)  Amino-acid sequence and oligosaccharide attachment sites of human erythrocyte glycophorin..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:1059087]
3. Huang, C H CH, Skov, F F, Daniels, G G, Tippett, P P and Blumenfeld, O O OO..  (1992)  Molecular analysis of human glycophorin MiIX gene shows a silent segment transfer and untemplated mutation resulting from gene conversion via sequence repeats..  Blood,    (1):   [PMID:1421409]
4. Huang, C H CH, Spruell, P P, Moulds, J J JJ and Blumenfeld, O O OO..  (1992)  Molecular basis for the human erythrocyte glycophorin specifying the Miltenberger class I (MiI) phenotype..  Blood,    (1):   [PMID:1611092]
5. Hamid, J J and Burness, A T AT..  (1990)  The mechanism of production of multiple mRNAs for human glycophorin A..  Nucleic acids research,    (11):   [PMID:2216775]
6. Dill, K K, Hu, S H SH, Berman, E E, Pavia, A A AA and Lacombe, J M JM..  (1990)  One- and two-dimensional NMR studies of the N-terminal portion of glycophorin A at 11.7 Tesla..  Journal of protein chemistry,      [PMID:2386609]
7. Kudo, S S and Fukuda, M M..  (1989)  Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2734312]
8. Tate, C G CG and Tanner, M J MJ..  (1988)  Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta..  The Biochemical journal,    (15):   [PMID:3196288]
9. Rahuel, C C and 8 more authors..  (1988)  Characterization of cDNA clones for human glycophorin A. Use for gene localization and for analysis of normal of glycophorin-A-deficient (Finnish type) genomic DNA..  European journal of biochemistry,    (15):   [PMID:3345758]
10. Siebert, P D PD and Fukuda, M M..  (1986)  Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3456608]
11. Siebert, P D PD and Fukuda, M M..  (1986)  Molecular biological study of the structure and expression of human glycophorin A..  Revue francaise de transfusion et immuno-hematologie,      [PMID:3809885]
12. Pasvol, G G, Wainscoat, J S JS and Weatherall, D J DJ..  (1982)  Erythrocytes deficiency in glycophorin resist invasion by the malarial parasite Plasmodium falciparum..  Nature,    (6):   [PMID:7040988]
13. Kudo, S S, Onda, M M and Fukuda, M M..  (1994)  Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals..  Journal of biochemistry,      [PMID:7798177]
14. Huang, C H CH, Reid, M M, Daniels, G G and Blumenfeld, O O OO..  (1993)  Alteration of splice site selection by an exon mutation in the human glycophorin A gene..  The Journal of biological chemistry,    (5):   [PMID:8245024]
15. Pisano, A A, Redmond, J W JW, Williams, K L KL and Gooley, A A AA..  (1993)  Glycosylation sites identified by solid-phase Edman degradation: O-linked glycosylation motifs on human glycophorin A..  Glycobiology,      [PMID:8286855]
16. Adams, P D PD, Engelman, D M DM and Brünger, A T AT..  (1996)  Improved prediction for the structure of the dimeric transmembrane domain of glycophorin A obtained through global searching..  Proteins,      [PMID:8953647]
17. MacKenzie, K R KR, Prestegard, J H JH and Engelman, D M DM..  (1997)  A transmembrane helix dimer: structure and implications..  Science (New York, N.Y.),    (4):   [PMID:9082985]
18. Poole, J and 7 more authors..  (1999)  Glycophorin A mutation Ala65 --> Pro gives rise to a novel pair of MNS alleles ENEP (MNS39) and HAG (MNS41) and altered Wrb expression: direct evidence for GPA/band 3 interaction necessary for normal Wrb expression..  Transfusion medicine (Oxford, England),      [PMID:10354388]
19. Storry, J R JR, Coghlan, G G, Poole, J J, Figueroa, D D and Reid, M E ME..  (2000)  The MNS blood group antigens, Vr (MNS12) and Mt(a) (MNS14), each arise from an amino acid substitution on glycophorin A..  Vox sanguinis,      [PMID:10729812]
20. Young, Mark T MT and Tanner, Michael J A MJ..  (2003)  Distinct regions of human glycophorin A enhance human red cell anion exchanger (band 3; AE1) transport function and surface trafficking..  The Journal of biological chemistry,    (29):   [PMID:12813056]
21. Hillier, Ladeana W LW and 121 more authors..  (2005)  Generation and annotation of the DNA sequences of human chromosomes 2 and 4..  Nature,    (7):   [PMID:15815621]
22. Yajima, Ayako A and 5 more authors..  (2008)  Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane..  Microbiology and immunology,      [PMID:18380804]
23. Pang, Allison J AJ and Reithmeier, Reinhart A F RA..  (2009)  Interaction of anion exchanger 1 and glycophorin A in human erythroleukaemic K562 cells..  The Biochemical journal,    (15):   [PMID:19438409]

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