| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab179692-10T (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10T | In stock | $49.90 | |
| Ab179692-25T | 25T | In stock | $82.90 | |
| Ab179692-100T | 100T | In stock | $209.90 |
mAb (HIR2); Mouse anti Human CD235a Antibody (FITC); Flow
| Product Name | CD235a Mouse mAb (FITC) - Primary antibody, specific to GYPA, Mouse IgG2b |
|---|---|
| Synonyms | MN sialoglycoprotein | PAS-2 | Sialoglycoprotein alpha | CD235a antigen | CD235a | glycophorin A (includes MN blood group) | glycophorin A (MNS blood group) | Glycophorin A | glycophorin Erik | glycophorin MiI | glycophorin MiIII | glycophorin MiV | glyco |
| Specifications & Purity | ExactAb™, Validated, Azide Free, Ex:498nm, Em:517nm, 5μL/test |
| Host species | Mouse |
| Specificity | GYPA |
| Immunogen | Synthetic peptide (Human, N-terminal) |
| Positive Control | Flow: K562 |
| Conjugation | FITC |
| Grade | Azide Free, Em:517nm, Ex:498nm, ExactAb™, Validated |
| Product Description | Application: |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 5μL/test |
| Storage Temp | Store at 2-8°C,Protected from light,Freeze prohibited |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
CD235a Mouse mAb (FITC) (Ab179692) - Flow Cytometry
Flow cytometry analysis of CD235a expression by K562 cells (red) compared to an isotype control (blue). Cells were stained with CD235a Mouse mAb (FITC) (Ab179692) at 0.3μg/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry |
5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. "> 5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303127 | Certificate of Analysis | Mar 12, 2024 | Ab179692 |
| ZJ24F0303128 | Certificate of Analysis | Mar 12, 2024 | Ab179692 |
| ZJ24F0303129 | Certificate of Analysis | Mar 12, 2024 | Ab179692 |
| 1. Bruce LJ, Pan RJ, Cope DL, Uchikawa M, Gunn RB, Cherry RJ, Tanner MJ. () Altered structure and anion transport properties of band 3 (AE1, SLC4A1) in human red cells lacking glycophorin A.. J Biol Chem, [PMID:14604989] [http://pubmed.ncbi.nlm.nih.gov/14604989] |
| 2. Tomita, M M and Marchesi, V T VT.. (1975) Amino-acid sequence and oligosaccharide attachment sites of human erythrocyte glycophorin.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:1059087] |
| 3. Huang, C H CH, Skov, F F, Daniels, G G, Tippett, P P and Blumenfeld, O O OO.. (1992) Molecular analysis of human glycophorin MiIX gene shows a silent segment transfer and untemplated mutation resulting from gene conversion via sequence repeats.. Blood, (1): [PMID:1421409] |
| 4. Huang, C H CH, Spruell, P P, Moulds, J J JJ and Blumenfeld, O O OO.. (1992) Molecular basis for the human erythrocyte glycophorin specifying the Miltenberger class I (MiI) phenotype.. Blood, (1): [PMID:1611092] |
| 5. Hamid, J J and Burness, A T AT.. (1990) The mechanism of production of multiple mRNAs for human glycophorin A.. Nucleic acids research, (11): [PMID:2216775] |
| 6. Dill, K K, Hu, S H SH, Berman, E E, Pavia, A A AA and Lacombe, J M JM.. (1990) One- and two-dimensional NMR studies of the N-terminal portion of glycophorin A at 11.7 Tesla.. Journal of protein chemistry, [PMID:2386609] |
| 7. Kudo, S S and Fukuda, M M.. (1989) Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2734312] |
| 8. Tate, C G CG and Tanner, M J MJ.. (1988) Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta.. The Biochemical journal, (15): [PMID:3196288] |
| 9. Rahuel, C C and 8 more authors.. (1988) Characterization of cDNA clones for human glycophorin A. Use for gene localization and for analysis of normal of glycophorin-A-deficient (Finnish type) genomic DNA.. European journal of biochemistry, (15): [PMID:3345758] |
| 10. Siebert, P D PD and Fukuda, M M.. (1986) Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3456608] |
| 11. Siebert, P D PD and Fukuda, M M.. (1986) Molecular biological study of the structure and expression of human glycophorin A.. Revue francaise de transfusion et immuno-hematologie, [PMID:3809885] |
| 12. Pasvol, G G, Wainscoat, J S JS and Weatherall, D J DJ.. (1982) Erythrocytes deficiency in glycophorin resist invasion by the malarial parasite Plasmodium falciparum.. Nature, (6): [PMID:7040988] |
| 13. Kudo, S S, Onda, M M and Fukuda, M M.. (1994) Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals.. Journal of biochemistry, [PMID:7798177] |
| 14. Huang, C H CH, Reid, M M, Daniels, G G and Blumenfeld, O O OO.. (1993) Alteration of splice site selection by an exon mutation in the human glycophorin A gene.. The Journal of biological chemistry, (5): [PMID:8245024] |
| 15. Pisano, A A, Redmond, J W JW, Williams, K L KL and Gooley, A A AA.. (1993) Glycosylation sites identified by solid-phase Edman degradation: O-linked glycosylation motifs on human glycophorin A.. Glycobiology, [PMID:8286855] |
| 16. Adams, P D PD, Engelman, D M DM and Brünger, A T AT.. (1996) Improved prediction for the structure of the dimeric transmembrane domain of glycophorin A obtained through global searching.. Proteins, [PMID:8953647] |
| 17. MacKenzie, K R KR, Prestegard, J H JH and Engelman, D M DM.. (1997) A transmembrane helix dimer: structure and implications.. Science (New York, N.Y.), (4): [PMID:9082985] |
| 18. Poole, J and 7 more authors.. (1999) Glycophorin A mutation Ala65 --> Pro gives rise to a novel pair of MNS alleles ENEP (MNS39) and HAG (MNS41) and altered Wrb expression: direct evidence for GPA/band 3 interaction necessary for normal Wrb expression.. Transfusion medicine (Oxford, England), [PMID:10354388] |
| 19. Storry, J R JR, Coghlan, G G, Poole, J J, Figueroa, D D and Reid, M E ME.. (2000) The MNS blood group antigens, Vr (MNS12) and Mt(a) (MNS14), each arise from an amino acid substitution on glycophorin A.. Vox sanguinis, [PMID:10729812] |
| 20. Young, Mark T MT and Tanner, Michael J A MJ.. (2003) Distinct regions of human glycophorin A enhance human red cell anion exchanger (band 3; AE1) transport function and surface trafficking.. The Journal of biological chemistry, (29): [PMID:12813056] |
| 21. Yajima, Ayako A and 5 more authors.. (2008) Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane.. Microbiology and immunology, [PMID:18380804] |
| 22. Pang, Allison J AJ and Reithmeier, Reinhart A F RA.. (2009) Interaction of anion exchanger 1 and glycophorin A in human erythroleukaemic K562 cells.. The Biochemical journal, (15): [PMID:19438409] |
