| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab211657-10μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab211657-25μg | 25μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $109.90 | |
| Ab211657-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $269.90 |
mAb (PC-61.5.3); Rat anti Mouse CD25 Antibody (AF555); Flow Cytometry
| Product Name | CD25 Rat mAb (AF555) |
|---|---|
| Specifications & Purity | ExactAb™, Validated, Ex:553nm, Em:568nm, 0.5 mg/mL |
| Host species | Rat |
| Specificity | CD25 |
| Immunogen | IL-2-dependent cytolytic mouse T cell clone B6.1 |
| Positive Control | Flow: C57BL/6 mouse splenocytes |
| Conjugation | Alexa Fluor® 555 |
| Grade | Em:568nm, Ex:553nm, ExactAb™, Validated |
| Product Description | Rat anti Mouse CD25 Antibody, Monoclonal (PC-61.5.3), could be used for Flow Cytometry |
| Light Chain Type | lambda |
|---|---|
| SDS-PAGE | 150kDa |
| Purification Method | Protein A purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Freeze prohibited |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
CD25 Rat mAb (AF555) (Ab211657) - Flow Cytometry
Con A-stimulated (2 days) C57BL/6 mouse splenocytes (right panel) stained with CD25 Rat mAb (AF555) (Ab211657) (red) compared to an isotype control (blue). Blue - Rat IgG (AF555) (Ab211666). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. ≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. |
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