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CD25 Rat mAb (APC) - Primary antibody, specific to CD25, IgG1

    Application:
  • Flow Cytometry
Features and benefitsView More
  • Host species: Rat
  • Species reactivity(Reacts with): Mouse
  • Isotype: Rat IgG1
  • Clone Number: PC-61.5.3
  • Conjugation: APC
Item Number
Ab179012
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab179012-10T (Trial Size)
Apply for free trial size(?)
Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
10T
≥10
$39.90
Ab179012-25T
25T
≥10
$59.90
Ab179012-100T
100T
6
$119.90

mAb(PC-61.5.3); Rat anti Mouse CD25 Antibody (APC); Flow

Basic Description

Product NameCD25 Rat mAb (APC) - Primary antibody, specific to CD25, IgG1
SynonymsAnti-mouse CD25 (IL-2Rα)-InVivo | In Vivo MAb anti-mouse CD25 (IL-2Rα) | CD25 Rat mAb | CD25, Il2r, Ly-43 | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2
Specifications & PurityExactAb™, Validated, Azide Free, Ex:650nm, Em:660nm, 5μL/test
Host speciesRat
SpecificityCD25
ImmunogenIL-2-dependent cytolytic mouse T cell clone B6.1
Positive ControlFlow: Murine splenocytes
ConjugationAPC
Excitation(nm)Red Laser(633nm)
Emission(nm)660nm
Ex/Em(nm)Ex:650nm;Em:660nm
GradeAzide Free, Em:660nm, Ex:650nm, ExactAb™, Validated
Product Description

Application:
Flow: 5μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume.

AI Insight

Product Properties

IsotypeIgG1
Light Chain Typelambda
Purification MethodProtein A High Salt
FormLiquid
Concentration5μL/test
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

CD25 Rat mAb (APC) (Ab179012) - Flow Cytometry
BALB/c mouse splenocytes are stained with CD25 Rat mAb (APC) (Ab179012) and CD4 Rat mAb (FITC) (Ab181111) (right). BALB/c mouse splenocytes are stained with CD4 Rat mAb (FITC) (Ab181111) and Rat IgG Isotype Control Antibody (APC) (Ab180313) (left).

CD25 Rat mAb (APC) (Ab179012) - Flow Cytometry
Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (APC) (Ab179012) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).

Associated Targets(Human)

IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
IL2RA Tclin Interleukin-2 receptor alpha chain (2 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

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Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
Flow Cytometry 5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. ">

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404893Certificate of AnalysisApr 25, 2024 Ab179012
ZJ24F0404892Certificate of AnalysisApr 25, 2024 Ab179012
ZJ24F0404891Certificate of AnalysisApr 25, 2024 Ab179012

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